General information for g851.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Matrix metalloproteinase-14.
Parent gene
- The parent gene g851 is located on chr_3: 6464176..6465442 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g851 | g851.t1 | isoform | g851.t1 | 6449545 | 6464176 |
| chr_3 | g851 | g851.t1 | exon | g851.t1.exon1 | 6449545 | 6449646 |
| chr_3 | g851 | g851.t1 | cds | g851.t1.CDS1 | 6449545 | 6449646 |
| chr_3 | g851 | g851.t1 | exon | g851.t1.exon2 | 6460057 | 6460189 |
| chr_3 | g851 | g851.t1 | cds | g851.t1.CDS2 | 6460057 | 6460189 |
| chr_3 | g851 | g851.t1 | exon | g851.t1.exon3 | 6460248 | 6460685 |
| chr_3 | g851 | g851.t1 | cds | g851.t1.CDS3 | 6460248 | 6460685 |
| chr_3 | g851 | g851.t1 | exon | g851.t1.exon4 | 6460757 | 6460872 |
| chr_3 | g851 | g851.t1 | cds | g851.t1.CDS4 | 6460757 | 6460872 |
| chr_3 | g851 | g851.t1 | exon | g851.t1.exon5 | 6460931 | 6461578 |
| chr_3 | g851 | g851.t1 | cds | g851.t1.CDS5 | 6460931 | 6461578 |
| chr_3 | g851 | g851.t1 | exon | g851.t1.exon6 | 6461645 | 6461783 |
| chr_3 | g851 | g851.t1 | cds | g851.t1.CDS6 | 6461645 | 6461783 |
| chr_3 | g851 | g851.t1 | exon | g851.t1.exon7 | 6464055 | 6464176 |
| chr_3 | g851 | g851.t1 | cds | g851.t1.CDS7 | 6464055 | 6464176 |
| chr_3 | g851 | g851.t1 | TTS | g851.t1 | 6464218 | 6464218 |
| chr_3 | g851 | g851.t1 | TSS | g851.t1 | NA | NA |
Sequences
>g851.t1 Gene=g851 Length=1698
ATGGATAAGAAATCAACAAAAATGTTCATTGCACTGATATTGACGACAACGCTCATGGCA
ACTCTTACAGAATCAGCACCCGCATCAATTAATCATGCTGAGATTTATCTTTCACAATTC
GGATATCTAAGTCCCAAAGCAAGAAATCCAAGTTCTGGAGGAAATTTATTAGCTCAAGAT
TCATGGGAAAATGCCATTAAAGAATTTCAGAGCTTTGCTGGTCTAAACATTACAGGTGAA
CTTGATGACGAGACAGTACAGACAATGGCACTGCCACGTTGTGGTGTGAAAGATAAAGTT
GGTTTCGGTTCAGACACAAGATCAAAGCGTTATGCTTTACAAGGTAGTCGTTGGAAGGTG
AAAGCTTTAACTTATAAAATTTCAAAATATCCAAAACGACTGAATAAGGATGATGTTGAT
AAAGAAGTAGCAAAAGCTTTTGCTGTTTGGAGCGAATATACAGACCTTACATTCACAGCA
AAGAAATCAAATCCAGTTCATATTGATATTAGATTTGAAACAGGCGAGCATGGTGATGGT
GATCCTTTTGATGGCCCAGGTGGCACTTTGGCTCACGCTTATTTCCCAGTTTATGGTGGT
GATGCGCATTTCGATGATGCCGAACAATGGACAATCAATAGTGCACGTGGAACTAATCTC
TTCCAAGTGGCAGCTCACGAATTTGGTCATAGTTTAGGATTGTCACATTCTGATGTCAGA
AGCGCTCTCATGGCTCCATTTTACAGAGGCTATGATCCTGTTTTTAGACTAGACTCTGAT
GACATTCAAGGAATTCAAGCACTTTATGGAAGGAAATCGCTTGCGTCAGGCTCTGGTTCG
GGAGCTGGCTCATCAGGACCATCAGCAAAAACAACAAAAGCACCAACAGTATTACGTGAT
GATGATTTACTTTGTAAAAATCCAAAGATCGATGCCATATTTAATACACCTGATGGTGCT
AGTTATGCTTTCAAAGAAGACAAATATTATAGACTCACAGAGAATGCAGTTGCAGAAGGT
TATCCAAAATTAATTTCAGAAGGATGGCCAGGACTGCCAAATAATATTGATGCTGCTTTT
ACTTATAAGAATGGCAAAACTTACTTCTTCAAAGGATCAAAATATTGGCGATATAATGGT
CGATCATTAGACGGTGATTATCCAAAAGAAATTAGTGAAGGATTTACTGGAGTGCCCGAT
AATGTTGATGCCGCAATGGTCTGGGGTGGAAATGGTAAAATTTACTTCTTCAAAGGCAGT
AAATTCTGGCGTTTTGATCCACTTAAAAGACCGCCAGTTAAATCAACATATCCAAAGCCA
GTCAGTAATTGGGAAGGCATTCCTGATAGCATTGATGCTGCTCTTCAGTATACCAATGGC
TACACTTACTTCTTTAAGGGTGATAAATATTATCGATTCAATGATCGAACATTCTCAATT
GATCAAGCTGATCCAGCGTTTCCAAGACCAACAGCTCATTGGTGGTTTGGTTGTAAAAAT
TCACCATCTCAGTTCTCAGGTAACTTTGAACTTCCTCCTGGAAGTGATGATGGTGTTGAT
CCACTTTTCGATGCAGATTCAAATGATCACAGACGGCCGCATGATGACAACAATGCTGCC
GTATCAGCACATTCAACGTTAATTAGTGTTCTTCTTCCCGCCATTTTCTTATCATCAATT
CGACTGTTATGGCATTAA
>g851.t1 Gene=g851 Length=565
MDKKSTKMFIALILTTTLMATLTESAPASINHAEIYLSQFGYLSPKARNPSSGGNLLAQD
SWENAIKEFQSFAGLNITGELDDETVQTMALPRCGVKDKVGFGSDTRSKRYALQGSRWKV
KALTYKISKYPKRLNKDDVDKEVAKAFAVWSEYTDLTFTAKKSNPVHIDIRFETGEHGDG
DPFDGPGGTLAHAYFPVYGGDAHFDDAEQWTINSARGTNLFQVAAHEFGHSLGLSHSDVR
SALMAPFYRGYDPVFRLDSDDIQGIQALYGRKSLASGSGSGAGSSGPSAKTTKAPTVLRD
DDLLCKNPKIDAIFNTPDGASYAFKEDKYYRLTENAVAEGYPKLISEGWPGLPNNIDAAF
TYKNGKTYFFKGSKYWRYNGRSLDGDYPKEISEGFTGVPDNVDAAMVWGGNGKIYFFKGS
KFWRFDPLKRPPVKSTYPKPVSNWEGIPDSIDAALQYTNGYTYFFKGDKYYRFNDRTFSI
DQADPAFPRPTAHWWFGCKNSPSQFSGNFELPPGSDDGVDPLFDADSNDHRRPHDDNNAA
VSAHSTLISVLLPAIFLSSIRLLWH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 30 | g851.t1 | CDD | cd04278 | ZnMc_MMP | 117 | 270 | 5.76376E-78 |
| 29 | g851.t1 | CDD | cd00094 | HX | 305 | 498 | 1.70582E-63 |
| 19 | g851.t1 | Gene3D | G3DSA:3.40.390.10 | Collagenase (Catalytic Domain) | 20 | 274 | 1.2E-87 |
| 20 | g851.t1 | Gene3D | G3DSA:2.110.10.10 | Hemopexin | 303 | 501 | 8.5E-72 |
| 7 | g851.t1 | PANTHER | PTHR10201 | MATRIX METALLOPROTEINASE | 28 | 485 | 2.3E-135 |
| 8 | g851.t1 | PANTHER | PTHR10201:SF271 | - | 28 | 485 | 2.3E-135 |
| 28 | g851.t1 | PIRSF | PIRSF001191 | MMP_stromelysin | 2 | 513 | 1.1E-170 |
| 11 | g851.t1 | PRINTS | PR00138 | Matrixin signature | 89 | 102 | 3.8E-40 |
| 12 | g851.t1 | PRINTS | PR00138 | Matrixin signature | 143 | 158 | 3.8E-40 |
| 10 | g851.t1 | PRINTS | PR00138 | Matrixin signature | 166 | 194 | 3.8E-40 |
| 9 | g851.t1 | PRINTS | PR00138 | Matrixin signature | 223 | 248 | 3.8E-40 |
| 13 | g851.t1 | PRINTS | PR00138 | Matrixin signature | 257 | 270 | 3.8E-40 |
| 1 | g851.t1 | Pfam | PF01471 | Putative peptidoglycan binding domain | 32 | 89 | 7.3E-10 |
| 2 | g851.t1 | Pfam | PF00413 | Matrixin | 117 | 270 | 1.2E-56 |
| 5 | g851.t1 | Pfam | PF00045 | Hemopexin | 310 | 353 | 6.8E-12 |
| 4 | g851.t1 | Pfam | PF00045 | Hemopexin | 356 | 399 | 1.2E-15 |
| 3 | g851.t1 | Pfam | PF00045 | Hemopexin | 402 | 448 | 2.5E-12 |
| 6 | g851.t1 | Pfam | PF00045 | Hemopexin | 451 | 498 | 1.1E-13 |
| 23 | g851.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 25 | - |
| 24 | g851.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 8 | - |
| 25 | g851.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 9 | 20 | - |
| 27 | g851.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 21 | 25 | - |
| 22 | g851.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 26 | 540 | - |
| 26 | g851.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 541 | 564 | - |
| 21 | g851.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 565 | 565 | - |
| 37 | g851.t1 | ProSitePatterns | PS00024 | Hemopexin domain signature. | 345 | 360 | - |
| 41 | g851.t1 | ProSiteProfiles | PS51642 | Hemopexin repeat profile. | 307 | 352 | 14.904 |
| 40 | g851.t1 | ProSiteProfiles | PS51642 | Hemopexin repeat profile. | 353 | 398 | 18.543 |
| 39 | g851.t1 | ProSiteProfiles | PS51642 | Hemopexin repeat profile. | 399 | 448 | 17.789 |
| 38 | g851.t1 | ProSiteProfiles | PS51642 | Hemopexin repeat profile. | 449 | 498 | 11.304 |
| 36 | g851.t1 | SMART | SM00235 | col_5 | 114 | 271 | 1.0E-51 |
| 32 | g851.t1 | SMART | SM00120 | HX_rpt_3 | 310 | 354 | 6.9E-10 |
| 34 | g851.t1 | SMART | SM00120 | HX_rpt_3 | 356 | 400 | 6.0E-14 |
| 33 | g851.t1 | SMART | SM00120 | HX_rpt_3 | 402 | 449 | 3.3E-12 |
| 35 | g851.t1 | SMART | SM00120 | HX_rpt_3 | 451 | 498 | 2.6E-11 |
| 14 | g851.t1 | SUPERFAMILY | SSF47090 | PGBD-like | 28 | 98 | 9.73E-17 |
| 16 | g851.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 110 | 271 | 1.1E-48 |
| 15 | g851.t1 | SUPERFAMILY | SSF50923 | Hemopexin-like domain | 303 | 499 | 1.7E-68 |
| 18 | g851.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 25 | - |
| 31 | g851.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 17 | g851.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 28 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0031012 | extracellular matrix | CC |
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0004222 | metalloendopeptidase activity | MF |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K07763 (matrix metalloproteinase-14 (membrane-inserted))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
