General information for g8512.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Ubiquitin-protein ligase E3B.
Parent gene
- The parent gene g8512 is located on chr_2: 31078849..31080843 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8512 | g8512.t2 | TSS | g8512.t2 | 31074777 | 31074777 |
| chr_2 | g8512 | g8512.t2 | isoform | g8512.t2 | 31074925 | 31075652 |
| chr_2 | g8512 | g8512.t2 | exon | g8512.t2.exon1 | 31074925 | 31075094 |
| chr_2 | g8512 | g8512.t2 | cds | g8512.t2.CDS1 | 31074925 | 31075094 |
| chr_2 | g8512 | g8512.t2 | exon | g8512.t2.exon2 | 31075152 | 31075652 |
| chr_2 | g8512 | g8512.t2 | cds | g8512.t2.CDS2 | 31075152 | 31075650 |
| chr_2 | g8512 | g8512.t2 | TTS | g8512.t2 | NA | NA |
Sequences
>g8512.t2 Gene=g8512 Length=671
ATGGATATTTCTGAAAATTCCAAGAATAAATTTGTCGAAAACATTCGTAAAAATCGAAGT
GATAGACATCAAGAAAAGGAAAAGGAGGAAAGAAGGCAACATGCTGCTATTCTAATTCAA
ACTATATATCGAGGCTTTATTGCGAGGAAAAAGTTTCGTGATGAAATTATCAAAAATTTC
AACGAAATTATTCCCGATTCTATTGATAAAGAAACAATTGAGCTGAAACCTGCTCTTCAA
ATTTATCATGCACTTTCACGTTTTCTATTAATATGGAAACCTAAAGAAGATGATGAAGAA
AGCAGAGAAAAATGGGAAACAATTTGTAGAATTTTGTTAGCCAGTGTTGAGTCAGAATCA
CTTAAATTTAATTACGTTTCTGTGGCATTAAATAAGGACCATAGTCTTGCATGGATAAGA
CACATGAAAAAAGTTCTTTTCTATTGTTGCACATTTATTGATTCATTAAAACCAGAAATT
CACAAGGATACAACATTACTTAATGTGTTGCTAAGGACTTTGATTGCATTCACATGTCCA
AACGGCTGGGCAATTTTAAAAACAAAGCAATTAGCACCAATGAAGCCTGCTATGCAAACA
ATTTGCAACAATATTGTGGGATTTCTTATTCAAAAAGGCTTCTATTTAACACTTCGAACG
ATATTAGTAAA
>g8512.t2 Gene=g8512 Length=223
MDISENSKNKFVENIRKNRSDRHQEKEKEERRQHAAILIQTIYRGFIARKKFRDEIIKNF
NEIIPDSIDKETIELKPALQIYHALSRFLLIWKPKEDDEESREKWETICRILLASVESES
LKFNYVSVALNKDHSLAWIRHMKKVLFYCCTFIDSLKPEIHKDTTLLNVLLRTLIAFTCP
NGWAILKTKQLAPMKPAMQTICNNIVGFLIQKGFYLTLRTILV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g8512.t2 | Gene3D | G3DSA:1.20.5.190 | - | 15 | 58 | 2.3E-5 |
| 5 | g8512.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 12 | 32 | - |
| 2 | g8512.t2 | PANTHER | PTHR45700:SF3 | UBIQUITIN-PROTEIN LIGASE E3B | 4 | 223 | 1.0E-44 |
| 3 | g8512.t2 | PANTHER | PTHR45700 | UBIQUITIN-PROTEIN LIGASE E3C | 4 | 223 | 1.0E-44 |
| 1 | g8512.t2 | Pfam | PF00612 | IQ calmodulin-binding motif | 34 | 53 | 2.5E-5 |
| 7 | g8512.t2 | ProSiteProfiles | PS50096 | IQ motif profile. | 32 | 61 | 9.633 |
| 4 | g8512.t2 | SMART | SM00015 | iq_5 | 31 | 53 | 2.4E-4 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed