General information for g8519.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Ubiquitin-conjugating enzyme E2-22 kDa.
Parent gene
- The parent gene g8519 is located on chr_2: 31115322..31116394 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8519 | g8519.t1 | TSS | g8519.t1 | 31111177 | 31111177 |
| chr_2 | g8519 | g8519.t1 | isoform | g8519.t1 | 31115322 | 31116394 |
| chr_2 | g8519 | g8519.t1 | exon | g8519.t1.exon1 | 31115322 | 31115828 |
| chr_2 | g8519 | g8519.t1 | cds | g8519.t1.CDS1 | 31115322 | 31115828 |
| chr_2 | g8519 | g8519.t1 | exon | g8519.t1.exon2 | 31116057 | 31116176 |
| chr_2 | g8519 | g8519.t1 | cds | g8519.t1.CDS2 | 31116057 | 31116176 |
| chr_2 | g8519 | g8519.t1 | exon | g8519.t1.exon3 | 31116236 | 31116394 |
| chr_2 | g8519 | g8519.t1 | cds | g8519.t1.CDS3 | 31116236 | 31116394 |
| chr_2 | g8519 | g8519.t1 | TTS | g8519.t1 | 31116548 | 31116548 |
Sequences
>g8519.t1 Gene=g8519 Length=786
ATGGACTTATTCACAAAGAGAATCAAGCGAAATTGGCTATTTTATCAAGACATGCCATCT
GCGAATCAATTGCATGAGGATTTTATGGACTTTATGAAGAATCGACCCGAATTCAATAGT
TTGATTAAAATTGGTAAAAGTTATATTCATAGCAAAGAATATTCAAGTGGTGTAGTAAGT
TTGCGATACAGCTGGAGGGATCAGTACGAGATACTGAAGGCAAATAATGTCTTTGCCGAA
TGCGCTTCAGAAGAGCTGGCATTAAAAATTGAGCGCGAATTACAGAATATGGCCATCAAG
TTGAATATTTTGATTCCTGGCAGTGATATGACAGAGGGACCATTAAGCAATCAACAAGAT
TATCGCTTCATTGTGTACATCACAAAACTCAAACCAATTGAAGATCGATGCTTGAGATGC
GAGAAATATTACTCGCAAGGTAAAAAATCTCACATGCTCAAGCATCATATGGGTTTTGTT
CGGGCATGGCGAGACTGCAGGCAGAGGAGAACAGTTCTGTTAAGCCTTCAAGCTCTTCTC
GCTGCTGCTGAACCAGATGATCCTCAAGATGCTGTTGTTGCTAATCAATATAAAGAAAAT
TACGAAATGTTTTGTAAAACTGCTAAGCATTGGACAAATGTTTATGCAAAAGGTCCACAT
AAAATTCCAGAATTTGATACAAAAGTGCAGACTCTTAAAAATATAGGAGTAAATGAATTT
GATGCAAGAGCTGCGTTAAGTCGACATAATTGGGATATTAATCTAGCAACCAATGATATA
TTCTGA
>g8519.t1 Gene=g8519 Length=261
MDLFTKRIKRNWLFYQDMPSANQLHEDFMDFMKNRPEFNSLIKIGKSYIHSKEYSSGVVS
LRYSWRDQYEILKANNVFAECASEELALKIERELQNMAIKLNILIPGSDMTEGPLSNQQD
YRFIVYITKLKPIEDRCLRCEKYYSQGKKSHMLKHHMGFVRAWRDCRQRRTVLLSLQALL
AAAEPDDPQDAVVANQYKENYEMFCKTAKHWTNVYAKGPHKIPEFDTKVQTLKNIGVNEF
DARAALSRHNWDINLATNDIF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g8519.t1 | Gene3D | G3DSA:3.10.110.10 | Ubiquitin Conjugating Enzyme | 141 | 220 | 0.0000000 |
| 2 | g8519.t1 | PANTHER | PTHR24068:SF164 | UBIQUITIN-CONJUGATING ENZYME E2 21-RELATED | 163 | 255 | 0.0000000 |
| 3 | g8519.t1 | PANTHER | PTHR24068 | UBIQUITIN-CONJUGATING ENZYME E2 | 163 | 255 | 0.0000000 |
| 1 | g8519.t1 | Pfam | PF00179 | Ubiquitin-conjugating enzyme | 162 | 211 | 0.0000024 |
| 7 | g8519.t1 | ProSiteProfiles | PS50127 | Ubiquitin-conjugating enzymes family profile. | 152 | 206 | 9.0250000 |
| 8 | g8519.t1 | ProSiteProfiles | PS50030 | Ubiquitin-associated domain (UBA) profile. | 224 | 261 | 8.5950000 |
| 4 | g8519.t1 | SUPERFAMILY | SSF54495 | UBC-like | 161 | 223 | 0.0000000 |
| 5 | g8519.t1 | SUPERFAMILY | SSF46934 | UBA-like | 223 | 261 | 0.0000287 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
