General information for g8532.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Lambda-crystallin-like protein.
Parent gene
- The parent gene g8532 is located on chr_2: 31139518..31140072 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8532 | g8532.t4 | isoform | g8532.t4 | 31138289 | 31139967 |
| chr_2 | g8532 | g8532.t4 | exon | g8532.t4.exon1 | 31138289 | 31138332 |
| chr_2 | g8532 | g8532.t4 | cds | g8532.t4.CDS1 | 31138289 | 31138332 |
| chr_2 | g8532 | g8532.t4 | exon | g8532.t4.exon2 | 31138944 | 31139430 |
| chr_2 | g8532 | g8532.t4 | cds | g8532.t4.CDS2 | 31138944 | 31139430 |
| chr_2 | g8532 | g8532.t4 | exon | g8532.t4.exon3 | 31139495 | 31139701 |
| chr_2 | g8532 | g8532.t4 | exon | g8532.t4.exon4 | 31139756 | 31139967 |
| chr_2 | g8532 | g8532.t4 | TTS | g8532.t4 | 31140081 | 31140081 |
| chr_2 | g8532 | g8532.t4 | TSS | g8532.t4 | NA | NA |
Sequences
>g8532.t4 Gene=g8532 Length=950
ATGGAGCCTATTTTAAAAGATCGTAAAATTGCAATTATCGGAAGCGGATTAATTGGGATG
TCATGGTCAATGATTTTTGCTTCAGCTGGTTATAAAGTTGTTATCTTTGATATTGTGCAA
TCACAAATTGATAATGCACTTAAACAGATCGAGTGTCAATTGAAGAGTTTAGAAGAAAGA
GGCTTATTACGTGGAAAGTTGACTGCAAAACAGCAATATAGCTGTATTTCAGGATGCACT
GATATCAAAGAAGCTGTAACAGGAGCTTTTTTTATGCAAGAGTGTGTCCCAGAAAATATT
GAATTGAAGAAAAAATTGTACAAACAATTGGATGAAATTGTTGATGATAAAATCATTATT
TCTTCATCAACTTCGACATTTATGCCTTCTATTTTCTCTGATGATATGAAACATCGTGCA
CAAGTTTTAGTTTCTCATCCAGTTAATCCACCTTACTATGTACCACTTGTTGAAATTGTT
CCTTCTAAACATACACGAAAAGATATTCCAACAATTACAAGAGCTTTGTAAAAGTTGGTC
AAAAACCTGTTGTTCTTTCTCGTGAAATTGAGGGATTTGCATTGAATCGAATTCAATATG
CAATTTTAAATGAATGCTGGCGTTTAATTGCTGATGGTATTTTGGATGTTAAAGATATTG
ATTCAGTAATGTCTGATGGATTGGGAATGAGATATGCTTTCCTAGGGCCAATGGAGACAG
CTCATTTAAATGCAGAAGGTTTCCTTTCATATTGCGATCGATATTCAAAGACAATTTATG
CTGTCAGTGAAACGATGGGACCAGTTCCGAAAATGGAAGGTCCCGTTATGGAGCAAGTTG
CTAAGGAATTGGAACAAATGTGTCCTCTTGAAGATTTAGAAAAACGCCGAAAGTGGCGCG
ACACATGTTTAACAGAGTTGAGTAAGATGAAAAAAGAATTAAATGATTGA
>g8532.t4 Gene=g8532 Length=176
MEPILKDRKIAIIGSGLIGMSWSMIFASAGYKVVIFDIVQSQIDNALKQIECQLKSLEER
GLLRGKLTAKQQYSCISGCTDIKEAVTGAFFMQECVPENIELKKKLYKQLDEIVDDKIII
SSSTSTFMPSIFSDDMKHRAQVLVSHPVNPPYYVPLVEIVPSKHTRKDIPTITRAL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g8532.t4 | Coils | Coil | Coil | 40 | 60 | - |
| 6 | g8532.t4 | Gene3D | G3DSA:3.40.50.720 | - | 5 | 176 | 7.2E-54 |
| 2 | g8532.t4 | PANTHER | PTHR48075 | 3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN | 10 | 175 | 8.1E-67 |
| 3 | g8532.t4 | PANTHER | PTHR48075:SF1 | LAMBDA-CRYSTALLIN HOMOLOG | 10 | 175 | 8.1E-67 |
| 1 | g8532.t4 | Pfam | PF02737 | 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain | 9 | 172 | 4.6E-44 |
| 8 | g8532.t4 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 11 | - |
| 10 | g8532.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 31 | - |
| 9 | g8532.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 32 | 176 | - |
| 5 | g8532.t4 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 7 | 172 | 9.56E-44 |
| 4 | g8532.t4 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 13 | 35 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0070403 | NAD+ binding | MF |
| GO:0006631 | fatty acid metabolic process | BP |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
