General information for g8532.t6
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Lambda-crystallin-like protein.
Parent gene
- The parent gene g8532 is located on chr_2: 31139518..31140072 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8532 | g8532.t6 | isoform | g8532.t6 | 31139101 | 31139967 |
| chr_2 | g8532 | g8532.t6 | exon | g8532.t6.exon1 | 31139101 | 31139426 |
| chr_2 | g8532 | g8532.t6 | cds | g8532.t6.CDS1 | 31139173 | 31139426 |
| chr_2 | g8532 | g8532.t6 | exon | g8532.t6.exon2 | 31139487 | 31139701 |
| chr_2 | g8532 | g8532.t6 | cds | g8532.t6.CDS2 | 31139487 | 31139701 |
| chr_2 | g8532 | g8532.t6 | exon | g8532.t6.exon3 | 31139761 | 31139967 |
| chr_2 | g8532 | g8532.t6 | cds | g8532.t6.CDS3 | 31139761 | 31139807 |
| chr_2 | g8532 | g8532.t6 | TTS | g8532.t6 | 31140081 | 31140081 |
| chr_2 | g8532 | g8532.t6 | TSS | g8532.t6 | NA | NA |
Sequences
>g8532.t6 Gene=g8532 Length=748
ACTGCAAAACAGCAATATAGCTGTATTTCAGGATGCACTGATATCAAAGAAGCTGTAACA
GGAGCTTTTTTTATGCAAGAGTGTGTCCCAGAAAATATTGAATTGAAGAAAAAATTGTAC
AAACAATTGGATGAAATTGTTGATGATAAAATCATTATTTCTTCATCAACTTCGACATTT
ATGCCTTCTATTTTCTCTGATGATATGAAACATCGTGCACAAGTTTTAGTTTCTCATCCA
GTTAATCCACCTTACTATGTACCACTTGTTGAAATTGTTCCTTCTAAACATACACGAAAA
GATATTCCAACAATTACAAGAGCTTTAATGACTGAAGTTGGTCAAAAACCTGTTGTTCTT
TCTCGTGAAATTGAGGGATTTGCATTGAATCGAATTCAATATGCAATTTTAAATGAATGC
TGGCGTTTAATTGCTGATGGTATTTTGGATGTTAAAGATATTGATTCAGTAATGTCTGAT
GGATTGGGAATGAGATATGCTTTCCTAGGGCCAATGGAGACAGCTCATTTAAATGCAGAA
GCTTTCATATTGCGATCGATATTCAAAGACAATTTATGCTGTCAGTGAAACGATGGGACC
AGTTCCGAAAATGGAAGGTCCCGTTATGGAGCAAGTTGCTAAGGAATTGGAACAAATGTG
TCCTCTTGAAGATTTAGAAAAACGCCGAAAGTGGCGCGACACATGTTTAACAGAGTTGAG
TAAGATGAAAAAAGAATTAAATGATTGA
>g8532.t6 Gene=g8532 Length=171
MQECVPENIELKKKLYKQLDEIVDDKIIISSSTSTFMPSIFSDDMKHRAQVLVSHPVNPP
YYVPLVEIVPSKHTRKDIPTITRALMTEVGQKPVVLSREIEGFALNRIQYAILNECWRLI
ADGILDVKDIDSVMSDGLGMRYAFLGPMETAHLNAEAFILRSIFKDNLCCQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8532.t6 | Gene3D | G3DSA:3.40.50.720 | - | 1 | 102 | 2.2E-31 |
| 9 | g8532.t6 | Gene3D | G3DSA:1.10.1040.10 | - | 103 | 165 | 1.3E-24 |
| 3 | g8532.t6 | PANTHER | PTHR48075 | 3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN | 1 | 158 | 4.9E-72 |
| 4 | g8532.t6 | PANTHER | PTHR48075:SF1 | LAMBDA-CRYSTALLIN HOMOLOG | 1 | 158 | 4.9E-72 |
| 2 | g8532.t6 | Pfam | PF02737 | 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain | 2 | 96 | 2.4E-27 |
| 1 | g8532.t6 | Pfam | PF00725 | 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain | 102 | 164 | 2.1E-14 |
| 7 | g8532.t6 | ProSitePatterns | PS00067 | 3-hydroxyacyl-CoA dehydrogenase signature. | 99 | 123 | - |
| 5 | g8532.t6 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 2 | 99 | 1.05E-22 |
| 6 | g8532.t6 | SUPERFAMILY | SSF48179 | 6-phosphogluconate dehydrogenase C-terminal domain-like | 102 | 155 | 1.11E-11 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | MF |
| GO:0070403 | NAD+ binding | MF |
| GO:0006631 | fatty acid metabolic process | BP |
| GO:0055114 | NA | NA |
| GO:0016491 | oxidoreductase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed