General information for g8541.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Pyrimidodiazepine synthase.
Parent gene
- The parent gene g8541 is located on chr_2: 31195530..31196506 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8541 | g8541.t1 | TSS | g8541.t1 | 31195210 | 31195210 |
| chr_2 | g8541 | g8541.t1 | isoform | g8541.t1 | 31195315 | 31196452 |
| chr_2 | g8541 | g8541.t1 | exon | g8541.t1.exon1 | 31195315 | 31195339 |
| chr_2 | g8541 | g8541.t1 | cds | g8541.t1.CDS1 | 31195315 | 31195339 |
| chr_2 | g8541 | g8541.t1 | exon | g8541.t1.exon2 | 31195532 | 31196053 |
| chr_2 | g8541 | g8541.t1 | cds | g8541.t1.CDS2 | 31195532 | 31196053 |
| chr_2 | g8541 | g8541.t1 | exon | g8541.t1.exon3 | 31196110 | 31196293 |
| chr_2 | g8541 | g8541.t1 | cds | g8541.t1.CDS3 | 31196110 | 31196293 |
| chr_2 | g8541 | g8541.t1 | exon | g8541.t1.exon4 | 31196419 | 31196452 |
| chr_2 | g8541 | g8541.t1 | cds | g8541.t1.CDS4 | 31196419 | 31196452 |
| chr_2 | g8541 | g8541.t1 | TTS | g8541.t1 | 31196549 | 31196549 |
Sequences
>g8541.t1 Gene=g8541 Length=765
ATGAGTAAATTACATTTATCAAAAGGTTCAACTCAACCAACTTTTCCTGATGATCAAAAG
CTTCGACTTTATTCAATGAGATTTTGTCCTTTCGCTCAACGTGCTCATCTCGTGCTCGAT
GCAAAAAATATTCCGTATCATACTGCCTTTATTAATTTAACAGAAAAGCCGGAATGGTTT
ACTAAAGTTTCTGCTTTGTCAAAAGTTCCAGCACTTGAAATTCCAGAAAGTGGCGATGTA
CTCATTGAATCTTTGATAATTGCTGACTATCTCGATGAAAAATATCCTCAAAATCCATTA
CACTCAAAAAATCCATTAGAAAAGGCTCGAGATAGAATTCTTATTGAAAGATTTAATGGA
TTTACTGCTCCATTTGCTCGAATTATATATAACCATAAGAAAGATGGAGCTCCGGGTGCG
ATTCATGAAATAGTCACAATTTTAGACTTGTTTGAAGAAGAACTTAAAAAGAGAGGAGCA
AAATTCTTTGGTGGTTCCAAACCAGGAATGCTCGATTATATGATTTGGCCTTGGTGTGAA
CGAACAGATATGTTAAAATATATGTTGGGCGATAAATATGAACTTGATAAAGTTCGATTT
AGTGAACTCATTAAATGGAAGGAGGAAATGAAAAAAGACAAAGCCGTTAAGGAACATTAT
ATTTCAGCCGAAGATCATTATAAATTTATATCATTGAGACTTGCAAATACTCCTGATTAT
GATTTTCTGGCGCCAACGGCTGCAAAAAAAATTCGCTTATCATAG
>g8541.t1 Gene=g8541 Length=254
MSKLHLSKGSTQPTFPDDQKLRLYSMRFCPFAQRAHLVLDAKNIPYHTAFINLTEKPEWF
TKVSALSKVPALEIPESGDVLIESLIIADYLDEKYPQNPLHSKNPLEKARDRILIERFNG
FTAPFARIIYNHKKDGAPGAIHEIVTILDLFEEELKKRGAKFFGGSKPGMLDYMIWPWCE
RTDMLKYMLGDKYELDKVRFSELIKWKEEMKKDKAVKEHYISAEDHYKFISLRLANTPDY
DFLAPTAAKKIRLS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g8541.t1 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 5 | 231 | 0.000 |
| 9 | g8541.t1 | Gene3D | G3DSA:1.20.1050.10 | - | 101 | 221 | 0.000 |
| 3 | g8541.t1 | PANTHER | PTHR43968 | - | 1 | 243 | 0.000 |
| 6 | g8541.t1 | PRINTS | PR01625 | Omega-class glutathione S-transferase signature | 19 | 34 | 0.000 |
| 4 | g8541.t1 | PRINTS | PR01625 | Omega-class glutathione S-transferase signature | 95 | 109 | 0.000 |
| 5 | g8541.t1 | PRINTS | PR01625 | Omega-class glutathione S-transferase signature | 162 | 181 | 0.000 |
| 2 | g8541.t1 | Pfam | PF13417 | Glutathione S-transferase, N-terminal domain | 23 | 99 | 0.000 |
| 1 | g8541.t1 | Pfam | PF13410 | Glutathione S-transferase, C-terminal domain | 143 | 189 | 0.000 |
| 12 | g8541.t1 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 19 | 99 | 20.094 |
| 11 | g8541.t1 | ProSiteProfiles | PS50405 | Soluble glutathione S-transferase C-terminal domain profile. | 104 | 242 | 16.574 |
| 13 | g8541.t1 | SFLD | SFLDG00358 | Main (cytGST) | 21 | 212 | 0.000 |
| 14 | g8541.t1 | SFLD | SFLDS00019 | Glutathione Transferase (cytosolic) | 21 | 212 | 0.000 |
| 7 | g8541.t1 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 20 | 121 | 0.000 |
| 8 | g8541.t1 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 103 | 242 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004364 | glutathione transferase activity | MF |
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005515 | protein binding | MF |
| GO:0005737 | cytoplasm | CC |
KEGG
Orthology
- This transcript has been annotated with K00799 (glutathione S-transferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
