General information for g8549.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Lipase member H-A.
Parent gene
- The parent gene g8549 is located on chr_2: 31251457..31258393 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8549 | g8549.t1 | isoform | g8549.t1 | 31251457 | 31258393 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon1 | 31251457 | 31251490 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS1 | 31251457 | 31251490 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon2 | 31251546 | 31251568 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS2 | 31251546 | 31251568 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon3 | 31251626 | 31251910 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS3 | 31251626 | 31251910 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon4 | 31251965 | 31252139 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS4 | 31251965 | 31252139 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon5 | 31252206 | 31252284 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS5 | 31252206 | 31252284 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon6 | 31252348 | 31252481 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS6 | 31252348 | 31252481 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon7 | 31252550 | 31252788 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS7 | 31252550 | 31252788 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon8 | 31252845 | 31253129 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS8 | 31252845 | 31253129 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon9 | 31253182 | 31253269 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS9 | 31253182 | 31253269 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon10 | 31253377 | 31253448 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS10 | 31253377 | 31253448 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon11 | 31253513 | 31253606 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS11 | 31253513 | 31253606 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon12 | 31257985 | 31258127 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS12 | 31257985 | 31258127 |
| chr_2 | g8549 | g8549.t1 | exon | g8549.t1.exon13 | 31258182 | 31258393 |
| chr_2 | g8549 | g8549.t1 | cds | g8549.t1.CDS13 | 31258182 | 31258393 |
| chr_2 | g8549 | g8549.t1 | TTS | g8549.t1 | 31259141 | 31259141 |
| chr_2 | g8549 | g8549.t1 | TSS | g8549.t1 | NA | NA |
Sequences
>g8549.t1 Gene=g8549 Length=1863
ATGAAATTTTTATTTTTTATTATTTGTCTGATTTTTTATGAAATCAATGCATTGACGGTC
GACGGTGGAATAAATTTAATATTCTTCGGCAAAAGTTTTACTGACATCACAACTACAACT
CTTGATTTCAATTTTGCAAGTCTTAAAGGCACAACGTATTTTGACATTAAAAAACCTACA
GCAGTTTTTGCTCATGGATATTTAACTGATTATAAAACACCAATGAATAAAGATCTTATC
AAAGCTTACATTTATCGAGGTGATTACAATGTGATCTCAATTGATTGGAGCAAATATTCA
TGGGATTGGAATATTTTTGCTGTTGCTAATTCTACTAATGATCAAGCAGATTATATAAGA
CAAATTTTACTTCAAATGAAATCTGCTGGTTTTGATTTGTCAACATTTCACTTTATTGGT
CATTGTGTTGGTGCTAATATTCTTGGTCGAGTTGGTTATCAATTTATTAAAAATAATACT
TTTACACTTACTCGAATTACTGGTTTAGATCCTTCCTCTGCATTTTTTGGAACAAGCAAT
GAATGGCCTGATAGTTGGAGTGAACTTTTTCCATCACTGAATAAGAAAAATGCAAAATTT
GTTGACATAATTCATACTGATGCTGGATTTCAAGGATTAAATTATGTTGGAGGTCATGCT
GACTTTTGGGTAAATGGTGGAAATAATCAACCAAAATGTTCATTTTTTGATATTTTCACT
TATCAAGCTGACGATATGATAAGATGTAGCCATAATCGTGCTGTGAAATATTATACAGAA
TCAGTTGAATCAACAACAAAGCTTCTTTTTATATCTACAAAATGTGTGAACAATACTTAT
ACAGCAGAAGCTTGCACTGGCTCAATTTCATCAATGGGAATTTATGCGAACAAATATGCA
GCAAATGAAGGCAATTTTTTTGTTTCTACTAATGGAACTTCGCCTTATTCAACTTCTAGT
CTTTTATTGATCGATGGTGGAATAAATTTAGTCTTTTTCGGAAAAGGTGCAAATGATTAT
AAATCTACAACATTAGATTATAATTATGCAAGTCTTAAAGGCACAAGGTATTTCGATATA
TCTAAACCTACAGCAGTTTATGCTCATGGATTTAATTCAAAATTTTTGGATCCTGATATA
ATGGGAATTGGTGAAGCATTTATTTCAAGAGAAGATCATAATGTCATTGCAGTTGATTGG
CAAAAATATACTTCAAATTTCTTTTATCCTGAAGTTGCTGGAGCTGTAGACGATGTAGCT
TCATTTATAGTTAAAATGTTAATACAAATGCAAACAGCTAGATATAATTTAACAACATTT
TACTTTATCGGTCATAGTTTAGGTGCTCAATTGATGGGACGTATTGGTTATCAACTCAAA
TCAAACTATAATTTCACAATATCTCGAATTACTGCACTTGACCCAGCTGGACCTCAGTTT
GGTAGTTTCAATGATTGGCCTACTTCCATTGCTTTTATTTTCCCAGCTGTCAATAAACTT
AACGCAAAATTCGTTGATGTGATTCATACTGATGCTGGATCGTTAGGTGATTCTTATTCT
GTGGCTCATGTTGACTTTTGGCCTAATGGTGGATTTGATCAGCCACTATGTAGCATTAGT
CCTACAAATTATAATTGTAATGCAACTTTTGATGTTTGCATTGGATGTGATCATGGTAGA
TCAATAAGATATTTTTCAGAATCAGTTAGATCGCTTACAAAGCATAAATTTAAATCATTT
TTGTGTTCAACTTCAATTATTGCTCCTGCAGCTTGCACTGGCTCAATTTCATCAATGGGT
TTTTATGCTAACTTGTATGCAGCAAATGAAGGCAATTATTATTGTCAAATCCTTCAGATT
TGA
>g8549.t1 Gene=g8549 Length=620
MKFLFFIICLIFYEINALTVDGGINLIFFGKSFTDITTTTLDFNFASLKGTTYFDIKKPT
AVFAHGYLTDYKTPMNKDLIKAYIYRGDYNVISIDWSKYSWDWNIFAVANSTNDQADYIR
QILLQMKSAGFDLSTFHFIGHCVGANILGRVGYQFIKNNTFTLTRITGLDPSSAFFGTSN
EWPDSWSELFPSLNKKNAKFVDIIHTDAGFQGLNYVGGHADFWVNGGNNQPKCSFFDIFT
YQADDMIRCSHNRAVKYYTESVESTTKLLFISTKCVNNTYTAEACTGSISSMGIYANKYA
ANEGNFFVSTNGTSPYSTSSLLLIDGGINLVFFGKGANDYKSTTLDYNYASLKGTRYFDI
SKPTAVYAHGFNSKFLDPDIMGIGEAFISREDHNVIAVDWQKYTSNFFYPEVAGAVDDVA
SFIVKMLIQMQTARYNLTTFYFIGHSLGAQLMGRIGYQLKSNYNFTISRITALDPAGPQF
GSFNDWPTSIAFIFPAVNKLNAKFVDVIHTDAGSLGDSYSVAHVDFWPNGGFDQPLCSIS
PTNYNCNATFDVCIGCDHGRSIRYFSESVRSLTKHKFKSFLCSTSIIAPAACTGSISSMG
FYANLYAANEGNYYCQILQI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g8549.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 21 | 318 | 1.7E-64 |
| 12 | g8549.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 327 | 619 | 6.1E-62 |
| 3 | g8549.t1 | PANTHER | PTHR11610 | LIPASE | 43 | 318 | 1.6E-115 |
| 4 | g8549.t1 | PANTHER | PTHR11610 | LIPASE | 339 | 616 | 1.6E-115 |
| 6 | g8549.t1 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 352 | 371 | 4.1E-7 |
| 8 | g8549.t1 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 396 | 411 | 4.1E-7 |
| 5 | g8549.t1 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 439 | 457 | 4.1E-7 |
| 7 | g8549.t1 | PRINTS | PR00821 | Triacylglycerol lipase family signature | 556 | 571 | 4.1E-7 |
| 1 | g8549.t1 | Pfam | PF00151 | Lipase | 53 | 316 | 1.7E-37 |
| 2 | g8549.t1 | Pfam | PF00151 | Lipase | 357 | 607 | 6.3E-40 |
| 15 | g8549.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 16 | g8549.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 17 | g8549.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 12 | - |
| 18 | g8549.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 14 | g8549.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 620 | - |
| 10 | g8549.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 40 | 316 | 1.66E-43 |
| 9 | g8549.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 343 | 584 | 6.1E-40 |
| 11 | g8549.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004806 | triglyceride lipase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed