General information for g8598.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase.
Parent gene
- The parent gene g8598 is located on chr_2: 31663874..31664397 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8598 | g8598.t3 | isoform | g8598.t3 | 31662266 | 31664397 |
| chr_2 | g8598 | g8598.t3 | exon | g8598.t3.exon1 | 31662266 | 31662767 |
| chr_2 | g8598 | g8598.t3 | TTS | g8598.t3 | 31662271 | 31662271 |
| chr_2 | g8598 | g8598.t3 | cds | g8598.t3.CDS1 | 31662657 | 31662767 |
| chr_2 | g8598 | g8598.t3 | exon | g8598.t3.exon2 | 31662841 | 31663102 |
| chr_2 | g8598 | g8598.t3 | cds | g8598.t3.CDS2 | 31662841 | 31663102 |
| chr_2 | g8598 | g8598.t3 | exon | g8598.t3.exon3 | 31663162 | 31663263 |
| chr_2 | g8598 | g8598.t3 | cds | g8598.t3.CDS3 | 31663162 | 31663263 |
| chr_2 | g8598 | g8598.t3 | exon | g8598.t3.exon4 | 31663545 | 31663623 |
| chr_2 | g8598 | g8598.t3 | cds | g8598.t3.CDS4 | 31663545 | 31663623 |
| chr_2 | g8598 | g8598.t3 | exon | g8598.t3.exon5 | 31663683 | 31663884 |
| chr_2 | g8598 | g8598.t3 | cds | g8598.t3.CDS5 | 31663683 | 31663884 |
| chr_2 | g8598 | g8598.t3 | exon | g8598.t3.exon6 | 31663945 | 31664397 |
| chr_2 | g8598 | g8598.t3 | cds | g8598.t3.CDS6 | 31663945 | 31664397 |
| chr_2 | g8598 | g8598.t3 | TSS | g8598.t3 | 31664495 | 31664495 |
Sequences
>g8598.t3 Gene=g8598 Length=1600
ATGAGAGCGAATAAACGTTTTCTACTTGCTCTTGGCATAATTGGTGTATGGTCGTTGCTC
ACATATCTAATTGTAATTAAAAATCACGATTATCAACAAAGTGGAAAATCAAAATTTCAC
GAGCGAATAATTTCACTAGAAAATGAAATTAGAAAAGAAACTGAAGATCGTAAAGACCTC
ATATCGCGATATCAAAATTTAATAAAAGTTCTAAGTGCACCAACAACTGCACTACCGATA
ATAAATGATAATAATATAAAAGAGAATGAAGTGGAACAGAATCAACTAAACAATAAAATA
AATTTCATTGGAAAGTATATTGATAATGATAACAATCGGCCTGTGATTCCAGTTATTGTT
ATCGCATGCAACCGCGTTTCAATTTCACGATCACTCGACCTTCTCATCAAATATCGTCCC
AATCGCGATCAATTTCCCATAATTGTGTCTCAGGATTGTGGTGATGAAGCAACACGTGAA
GTAATTTTATCATATAAGGATGAAGTGCAATTAGTACAGCAGCCAGATTTAAGTGACATT
GAAGTTCCACCAAAGGATAAAAAATTCAAAGGATATTATAAAATTTCTCGTCATTACAAT
TGGGCCTTAAATCTTGTGTTCTCTTATGGCTATGAATTTGTGATCATCGTTGAAGATGAC
CTTGATGTGGCACCCGACTTTTATGAATACTTTTTAGGAACGTTTCCACTTCTACGTGCT
GATAATACTTTATGTTGGGATGATTGGATAAGAAATCCAGAACAGAGACTGAATAGAGCA
TGCATACGACCAGAGTTGCCGCGAACAAGAACATTCGGAAAAATTGGGGTTTCAAATGGT
TTATTCTTTGAAAAACATTTAAAATATATAAAATTAAGTGAAACTCCAGTTGAATTTTCA
AAAAAAGATTTGAGTTATTTATTAAAAGAGAATTATGATAAACAATTTGTCGAAGAAGTT
TATAAAATTCCTGTTGTGACATTTGAGGAATTAAGGAAAGGTCTTGTGAAAGTTGATGGG
CCTGTGAGAATACAATACAATAATAGAAATCAATATAAATTAGCGGCAAAGAATTTAGGA
ATCATGGAAGATTTTAAGAGTGGTGTTCCAAGAACAGCTTATGCTGGCATCGTATCAGCA
TTTTATCAAAATCGTCGTGTATATTTAGCGCCAAGTGCAAATTGGAAAGGCTACGATCCA
TCATGGTGATGATAGTGGTAATTAAAAATAATAATTTTGTCCATATATAAAAAATTAGAT
TGAAAGCCAAAATGAAATGAAAATGAATGTTTTTATATTAAAAATATATAATATACATAC
TGACTGCATGACATATTTTTATGCTTCAAAATATGAAATGAAATTTAACACCATTGCTAA
TTACCTTACTTATAAAAAAGTGTGAGAAAATTGACTATAAATGCGCAACAGTTTCTTCTT
TACACAAGTGTGTTCTGAATATTTCACTTTTATCTTTTTATAGTCAAAATATTGAGTGAG
AAATTTCTGCTTTTTTGCTCAATTTCTAATTGTTTTCCGCAAAAAATATTGCAGAATTTT
TTGTATTGCGCAATGAAAATAGAAAGAAGTCAGCAAAAAA
>g8598.t3 Gene=g8598 Length=402
MRANKRFLLALGIIGVWSLLTYLIVIKNHDYQQSGKSKFHERIISLENEIRKETEDRKDL
ISRYQNLIKVLSAPTTALPIINDNNIKENEVEQNQLNNKINFIGKYIDNDNNRPVIPVIV
IACNRVSISRSLDLLIKYRPNRDQFPIIVSQDCGDEATREVILSYKDEVQLVQQPDLSDI
EVPPKDKKFKGYYKISRHYNWALNLVFSYGYEFVIIVEDDLDVAPDFYEYFLGTFPLLRA
DNTLCWDDWIRNPEQRLNRACIRPELPRTRTFGKIGVSNGLFFEKHLKYIKLSETPVEFS
KKDLSYLLKENYDKQFVEEVYKIPVVTFEELRKGLVKVDGPVRIQYNNRNQYKLAAKNLG
IMEDFKSGVPRTAYAGIVSAFYQNRRVYLAPSANWKGYDPSW
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g8598.t3 | Coils | Coil | Coil | 36 | 63 | - |
| 10 | g8598.t3 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 114 | 244 | 7.0E-45 |
| 9 | g8598.t3 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 245 | 287 | 3.3E-13 |
| 11 | g8598.t3 | Gene3D | G3DSA:3.10.180.20 | - | 323 | 402 | 9.7E-32 |
| 4 | g8598.t3 | PANTHER | PTHR10468 | PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1/ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE | 36 | 244 | 2.8E-108 |
| 6 | g8598.t3 | PANTHER | PTHR10468:SF0 | ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE | 36 | 244 | 2.8E-108 |
| 3 | g8598.t3 | PANTHER | PTHR10468 | PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1/ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE | 246 | 398 | 2.8E-108 |
| 5 | g8598.t3 | PANTHER | PTHR10468:SF0 | ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE | 246 | 398 | 2.8E-108 |
| 2 | g8598.t3 | Pfam | PF03071 | GNT-I family | 106 | 244 | 1.8E-50 |
| 1 | g8598.t3 | Pfam | PF03071 | GNT-I family | 246 | 402 | 4.3E-58 |
| 13 | g8598.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 15 | g8598.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
| 14 | g8598.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 27 | 402 | - |
| 8 | g8598.t3 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 113 | 402 | 3.56E-44 |
| 7 | g8598.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008375 | acetylglucosaminyltransferase activity | MF |
| GO:0006486 | protein glycosylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
