General information for g8608.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cyclin-dependent kinase 14.
Parent gene
- The parent gene g8608 is located on chr_2: 31758306..31760969 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8608 | g8608.t1 | TSS | g8608.t1 | 31757067 | 31757067 |
| chr_2 | g8608 | g8608.t1 | isoform | g8608.t1 | 31757349 | 31760969 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon1 | 31757349 | 31757418 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS1 | 31757349 | 31757418 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon2 | 31758310 | 31758387 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS2 | 31758310 | 31758387 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon3 | 31758691 | 31758840 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS3 | 31758691 | 31758840 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon4 | 31758922 | 31759121 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS4 | 31758922 | 31759121 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon5 | 31759210 | 31759301 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS5 | 31759210 | 31759301 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon6 | 31759375 | 31759549 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS6 | 31759375 | 31759549 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon7 | 31759862 | 31760016 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS7 | 31759862 | 31760016 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon8 | 31760107 | 31760213 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS8 | 31760107 | 31760213 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon9 | 31760290 | 31760502 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS9 | 31760290 | 31760502 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon10 | 31760577 | 31760665 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS10 | 31760577 | 31760665 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon11 | 31760724 | 31760814 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS11 | 31760724 | 31760814 |
| chr_2 | g8608 | g8608.t1 | exon | g8608.t1.exon12 | 31760899 | 31760969 |
| chr_2 | g8608 | g8608.t1 | cds | g8608.t1.CDS12 | 31760899 | 31760969 |
| chr_2 | g8608 | g8608.t1 | TTS | g8608.t1 | 31761749 | 31761749 |
Sequences
>g8608.t1 Gene=g8608 Length=1491
ATGTATTCTACGATGGATAAACAATCATCGAGTAGTAACAATTTGAAAATGACTAATACT
TCAGCTACGGGTGTCACAATGCGTGAGAAAAAAAGTGGAGCTCTGCAGAAATTAAAAAAG
CGATTATCACATAGCTTTGGAAGATTATCAATATCAAGAGAAGATAATGATGAGTCAAAT
AATCACCATAGTCAAAACAGAAATAATAGTAAAATGCCATACAATAATGGATATTCCGAA
GAATATTTAGATCGCTTGGAGAAAAATGGTAATATTCAGGCTGATAACAAAGCTTACAAT
TGGTCTGGTAATCATAATCATGGTCAAGTTCATAGGCAATTATCGGTATCATCAGACAGT
AAGCTTCTCGATGATGATATAAGAGAAGAAACGCGTGTGATAATGAGACCCAAAAAAATT
CCACCGCGACCTAAGTCAGAAGTGTTCTTAAATAAGCACGATTCTCATCCGAGAAGGAAA
CGATTCAGTGCATTTGGGGGCGACTCGCCATTTGGTAAATCGGAGGCATATGTTAAACTG
GAGCAACTTGGCGAGGGCTCATATGCAACAGTATTCAAGGGTTTTAGCAACCTCACAAAT
CAAGTCGTTGCGCTAAAAGAAATAAGACTACAAGAGGAAGAAGGTGCACCATTCACAGCC
ATTCGAGAAGCATCTCTTTTAAAAGAATTGAAACATGCCAATATTGTAACGCTTCATGAT
ATTGTTCACACAAGAGAAACATTGACATTTGTATTTGAATATGTGAATACTGATCTTTCA
CAATATATGGAACGACATCCTGGCGGCCTCGATTATAGAAATATCAAGCTATTTCTCTTC
CAATTATTACGCGGCCTCACATATTGCCATAAAAGGAGAGTCTTACATAGAGATGTTAAG
CCTCAAAATTTGCTCATAAGTGAAATTGGTGAATTGAAGTTAGCCGATTTTGGTTTAGCA
AGAGCAAAGAGCGTTCCAAGTCATACTTATTCACATGAAGTTGTTACACTATGGTATCGA
CCACCAGATGTTCTTCTCGGGAGCACAGACTATTCGACATCGCTCGATATGTGGGGCGTT
GGTTGTATTTTCATAGAAATGATAACGGGTATGCCTACCTTCCCCGGGATTCGTGATACA
TACGATCAATTGGACAAAATTTTCAAACTTCTCGGCACACCGACTGAAGAGATTTGGTCG
GGCGTCACGCATTTACCGAGCTATAAACCACACAAATTAGGCTTTTATCGACCTCGCAAA
TTAGGCTTAAGTTTTCCACGTCTTTATGATATTCCAGAAGGAGAAGCAATGGCTAGTGCA
TTATTACAGCTAAATCCAAACAATCGAATAGGGGCAGAAGCAGCTTTAAAGCATCCTTAT
TTTGCCCATTTGCCTAAGAAACTTTATGAACTTCCCGATGAAACGTCAATATTCAATGTC
GAGGGTGTGTTTCTACACCCCGAGCCAAATCGTGAGCGACACAACAACTGA
>g8608.t1 Gene=g8608 Length=496
MYSTMDKQSSSSNNLKMTNTSATGVTMREKKSGALQKLKKRLSHSFGRLSISREDNDESN
NHHSQNRNNSKMPYNNGYSEEYLDRLEKNGNIQADNKAYNWSGNHNHGQVHRQLSVSSDS
KLLDDDIREETRVIMRPKKIPPRPKSEVFLNKHDSHPRRKRFSAFGGDSPFGKSEAYVKL
EQLGEGSYATVFKGFSNLTNQVVALKEIRLQEEEGAPFTAIREASLLKELKHANIVTLHD
IVHTRETLTFVFEYVNTDLSQYMERHPGGLDYRNIKLFLFQLLRGLTYCHKRRVLHRDVK
PQNLLISEIGELKLADFGLARAKSVPSHTYSHEVVTLWYRPPDVLLGSTDYSTSLDMWGV
GCIFIEMITGMPTFPGIRDTYDQLDKIFKLLGTPTEEIWSGVTHLPSYKPHKLGFYRPRK
LGLSFPRLYDIPEGEAMASALLQLNPNNRIGAEAALKHPYFAHLPKKLYELPDETSIFNV
EGVFLHPEPNRERHNN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g8608.t1 | CDD | cd07844 | STKc_PCTAIRE_like | 176 | 461 | 0.0 |
| 5 | g8608.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 174 | 256 | 2.8E-31 |
| 6 | g8608.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 257 | 495 | 9.4E-63 |
| 13 | g8608.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 27 | - |
| 14 | g8608.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 75 | - |
| 12 | g8608.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 47 | 64 | - |
| 2 | g8608.t1 | PANTHER | PTHR24056:SF189 | ECDYSONE-INDUCED PROTEIN 63E, ISOFORM N | 132 | 488 | 8.7E-179 |
| 3 | g8608.t1 | PANTHER | PTHR24056 | CELL DIVISION PROTEIN KINASE | 132 | 488 | 8.7E-179 |
| 1 | g8608.t1 | Pfam | PF00069 | Protein kinase domain | 177 | 461 | 3.6E-67 |
| 10 | g8608.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 183 | 206 | - |
| 9 | g8608.t1 | ProSitePatterns | PS00191 | Cytochrome b5 family, heme-binding domain signature. | 262 | 269 | - |
| 8 | g8608.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 294 | 306 | - |
| 15 | g8608.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 177 | 461 | 43.707 |
| 11 | g8608.t1 | SMART | SM00220 | serkin_6 | 177 | 461 | 7.1E-88 |
| 4 | g8608.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 174 | 466 | 5.8E-88 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K08821 (cyclin-dependent kinase 14)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
