General information for g8642.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine/threonine-protein kinase N.
Parent gene
- The parent gene g8642 is located on chr_2: 32015258..32016656 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8642 | g8642.t1 | isoform | g8642.t1 | 32004419 | 32009464 |
| chr_2 | g8642 | g8642.t1 | exon | g8642.t1.exon1 | 32004419 | 32004499 |
| chr_2 | g8642 | g8642.t1 | cds | g8642.t1.CDS1 | 32004419 | 32004499 |
| chr_2 | g8642 | g8642.t1 | exon | g8642.t1.exon2 | 32005331 | 32005504 |
| chr_2 | g8642 | g8642.t1 | cds | g8642.t1.CDS2 | 32005331 | 32005504 |
| chr_2 | g8642 | g8642.t1 | exon | g8642.t1.exon3 | 32005582 | 32005871 |
| chr_2 | g8642 | g8642.t1 | cds | g8642.t1.CDS3 | 32005582 | 32005871 |
| chr_2 | g8642 | g8642.t1 | exon | g8642.t1.exon4 | 32007540 | 32007708 |
| chr_2 | g8642 | g8642.t1 | cds | g8642.t1.CDS4 | 32007540 | 32007708 |
| chr_2 | g8642 | g8642.t1 | exon | g8642.t1.exon5 | 32007774 | 32007911 |
| chr_2 | g8642 | g8642.t1 | cds | g8642.t1.CDS5 | 32007774 | 32007911 |
| chr_2 | g8642 | g8642.t1 | exon | g8642.t1.exon6 | 32009318 | 32009464 |
| chr_2 | g8642 | g8642.t1 | cds | g8642.t1.CDS6 | 32009318 | 32009464 |
| chr_2 | g8642 | g8642.t1 | TSS | g8642.t1 | 32009989 | 32009989 |
| chr_2 | g8642 | g8642.t1 | TTS | g8642.t1 | NA | NA |
Sequences
>g8642.t1 Gene=g8642 Length=999
ATGAAAAATATGCTGGATAATAAAATGACTAAATATAAATCTGTCAAGGACTTGCATATA
ATTGGCAGTAATCATAGTAACAGCACAAAACGAAGTGGTAATAGTGTGCTGAATGCCTGT
ATTAAATTGGGTAACCCTTTCGATGAGAATGAATACATTAAATATCCAGTTCTGTATGAG
CTCAGTCACAAGTATTTAAATGACAATCAGCCAGAAGCCAATTTACCTGATAGACTAGAA
GAACTCAAAGAGGCCATTCGGAAAGAAATTCGAAAAGAGCTAAAGATCAAGGAAGGTGCT
GAAAAGTTGCGAGAAGTAGCAACTGATAGAAGAGCATTATCCGATGTTGCCTCAATTGTA
AAAAAAAGCAACACAAAGCTACAAGAACTCAAGTCTGAACTGCATGAACTCGAGAGCCAA
ATTATATTGACGCAGGCAAATAGCAATGGAAAAGACTATCTCAATTCATCTCCGCCTGCA
AAGAATTCAAATTCAGAAACTCAAACTGACAACTCCGAAGCATCTAATGACAAACATTTA
CAATCACTTGAAAAGCAGTTGACGATTGAAATGAAAGTCAAAAATGGGACAGAAAACATG
ATTCAATCAATATCACAAAATTATCAAAGTCGTGATAAAAAGCTGCTCGCTGAAGCATAT
ACAATGCTTGAAGATTCAAAAGCGAAAATTGAATTCCTTAAATTGAAAATTTTGAAGGTC
AAACAGAATCGACAGATGAATAAAATATCAGAGAATGGAGAAGTGAGATCGAATGAGCAG
CTTGAGACATCATTGGAAGAACGAATTGAAGAACTACGCCATCGACTAAGAATCGAAGCT
GCCGTCGTTGATGGTGCAAAAAATGTGATTAGGACTCTTCAAAGTACTAAAACAATTGAC
AAGAAAGCCTTACAAGAGTTAAAGACTCTCGCGGCGAGAGCTGTTCAGTCACTTGTTACG
TTTGTTGAAAATCAGAAGTTGCCATTTATTCGTACTTAA
>g8642.t1 Gene=g8642 Length=332
MKNMLDNKMTKYKSVKDLHIIGSNHSNSTKRSGNSVLNACIKLGNPFDENEYIKYPVLYE
LSHKYLNDNQPEANLPDRLEELKEAIRKEIRKELKIKEGAEKLREVATDRRALSDVASIV
KKSNTKLQELKSELHELESQIILTQANSNGKDYLNSSPPAKNSNSETQTDNSEASNDKHL
QSLEKQLTIEMKVKNGTENMIQSISQNYQSRDKKLLAEAYTMLEDSKAKIEFLKLKILKV
KQNRQMNKISENGEVRSNEQLETSLEERIEELRHRLRIEAAVVDGAKNVIRTLQSTKTID
KKALQELKTLAARAVQSLVTFVENQKLPFIRT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g8642.t1 | CDD | cd11622 | HR1_PKN_1 | 78 | 141 | 1.1655E-21 |
| 16 | g8642.t1 | CDD | cd11623 | HR1_PKN_2 | 173 | 242 | 6.72516E-23 |
| 14 | g8642.t1 | Coils | Coil | Coil | 120 | 147 | - |
| 12 | g8642.t1 | Coils | Coil | Coil | 223 | 243 | - |
| 13 | g8642.t1 | Coils | Coil | Coil | 255 | 275 | - |
| 11 | g8642.t1 | Gene3D | G3DSA:1.10.287.160 | HR1 repeat | 59 | 141 | 4.3E-26 |
| 9 | g8642.t1 | Gene3D | G3DSA:1.10.287.160 | HR1 repeat | 171 | 250 | 6.5E-23 |
| 10 | g8642.t1 | Gene3D | G3DSA:1.10.287.160 | HR1 repeat | 260 | 317 | 3.5E-13 |
| 20 | g8642.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 153 | 176 | - |
| 21 | g8642.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 153 | 178 | - |
| 4 | g8642.t1 | PANTHER | PTHR24356 | SERINE/THREONINE-PROTEIN KINASE | 72 | 304 | 3.5E-18 |
| 5 | g8642.t1 | PANTHER | PTHR24356:SF387 | - | 72 | 304 | 3.5E-18 |
| 3 | g8642.t1 | Pfam | PF02185 | Hr1 repeat | 84 | 140 | 3.4E-12 |
| 2 | g8642.t1 | Pfam | PF02185 | Hr1 repeat | 180 | 245 | 7.7E-14 |
| 1 | g8642.t1 | Pfam | PF02185 | Hr1 repeat | 268 | 309 | 2.2E-8 |
| 24 | g8642.t1 | ProSiteProfiles | PS51860 | REM-1 domain profile. | 68 | 143 | 22.084 |
| 23 | g8642.t1 | ProSiteProfiles | PS51860 | REM-1 domain profile. | 164 | 246 | 19.38 |
| 22 | g8642.t1 | ProSiteProfiles | PS51860 | REM-1 domain profile. | 247 | 332 | 8.904 |
| 19 | g8642.t1 | SMART | SM00742 | hr1_7 | 81 | 144 | 1.7E-15 |
| 17 | g8642.t1 | SMART | SM00742 | hr1_7 | 178 | 247 | 4.3E-13 |
| 18 | g8642.t1 | SMART | SM00742 | hr1_7 | 267 | 329 | 0.0038 |
| 8 | g8642.t1 | SUPERFAMILY | SSF46585 | HR1 repeat | 65 | 140 | 5.23E-17 |
| 6 | g8642.t1 | SUPERFAMILY | SSF46585 | HR1 repeat | 175 | 246 | 5.75E-17 |
| 7 | g8642.t1 | SUPERFAMILY | SSF46585 | HR1 repeat | 262 | 309 | 3.01E-11 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0031267 | small GTPase binding | MF |
| GO:0007165 | signal transduction | BP |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
