General information for g8661.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable chitinase 10.
Parent gene
- The parent gene g8661 is located on chr_2: 32137821..32143743 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8661 | g8661.t1 | isoform | g8661.t1 | 32137821 | 32143743 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon1 | 32137821 | 32137862 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS1 | 32137821 | 32137862 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon2 | 32139163 | 32139241 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS2 | 32139163 | 32139241 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon3 | 32139728 | 32140209 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS3 | 32139728 | 32140209 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon4 | 32140393 | 32140548 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS4 | 32140393 | 32140548 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon5 | 32140612 | 32140750 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS5 | 32140612 | 32140750 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon6 | 32140811 | 32141306 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS6 | 32140811 | 32141306 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon7 | 32141712 | 32141874 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS7 | 32141712 | 32141874 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon8 | 32141926 | 32142040 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS8 | 32141926 | 32142040 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon9 | 32142104 | 32142445 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS9 | 32142104 | 32142445 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon10 | 32142506 | 32143134 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS10 | 32142506 | 32143134 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon11 | 32143275 | 32143476 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS11 | 32143275 | 32143476 |
| chr_2 | g8661 | g8661.t1 | exon | g8661.t1.exon12 | 32143535 | 32143743 |
| chr_2 | g8661 | g8661.t1 | cds | g8661.t1.CDS12 | 32143535 | 32143743 |
| chr_2 | g8661 | g8661.t1 | TSS | g8661.t1 | NA | NA |
| chr_2 | g8661 | g8661.t1 | TTS | g8661.t1 | NA | NA |
Sequences
>g8661.t1 Gene=g8661 Length=3054
ATGAATGAAGGTTATTGCATGCATCCGATGTTGGAACACAATCTACCACGACTGTTTCGA
TTAGCGTTTCTAGCCTGCATATTATTGCTTATATTTTCACATTTAACAGACAGTGCCCAA
GAGAAAAGAAGAATACGAAGGCCAACGAGCTTAGCAAATAAAAATACAGAAAAATCGTCG
TCATTAAGAAATGCCACGAGAAGTACAAGTTCGATATTGAAGAAAGTGGCGCAAGATAGA
GTGGATTTGGCGCCTGCACCATCAACTAATCGATTCAAAGTTCGACAACGAAGGCCATCA
ACAGCTCTTGCTGCATCAGCGTCAAATGACAATTCCGTTTCAACATCAAATGAAGGCATT
AAAGATAAGGATGGTTATAAAATTGTCTGCTATTACACAAATTGGTCACAATATCGTGTA
AAGATTGGTAAGTTCTTACCTGAAGATATTCCAGCTGATTTGTGCACGCACATCATTTTT
GCCTTTGGTTGGTTGAAGAAGGGCAAATTGAGCTCATTTGAAAGCAATGATGAAACAAAG
GACGGAAAAGTTGGATTATATCAACGAATTGTTGGCCTTAAAAAAGCTAATCCAAATCTT
AAGGTTCTTTTAGCCATTGGCGGTTGGTCATTTGGAACTCAAAAATTCAAAGAAATGTCA
AAATCACGTTATGCTCGTCAGACCTTCATCTATTCAGCTATTCCATATTTGAGACAACGT
GGATTTGATGGACTTGATTTAGATTGGGAATATCCAGCTGGATCAGATGATAAGAAAAAC
TTTGTTCTTCTTCTTAAAGAATTAAGAGAATCATTTGAAGCTGAATCACAAGAAATTCGT
CAACCTCGTTTACTTTTATCAGCTGCTGTGCCAGTTGGTCCTGATAACATTCGAGGAGGA
TATGATGTACCTGCAGTTGCAAGTTATCTTGATTTTATTAATTTAATGGCTTACGATTTT
CATGGAAAATGGGAAAGAGAAACTGGTCATAATGCTCCACTTTATGCACCATCAACTGAT
TCAGAATGGCGTAAACAATTATCAGTTGATAATGCTGCTAATTTGTGGGTTAAAATGGGC
GCACCAAAAGAAAAATTGATTATCGGTATGCCAACTTATGGACGTTCATTCACTCTCGCT
AATACAGACAAACATGGACCAAATTCACCTGCTAGTGGCGGTGGACGTGAAGGAGTTTAT
ACAAAAGAAAGTGGCTTTTTGGCTTATTATGAAATTTGTGAAATGTTGAATAATGGTGCA
GTTTATGTTTGGGATGAAGAAATGAAAGTTCCTTACATGGTCGATGGTGATCAATGGGTT
GGTTTTGATGATGAAAGAGCAATAAGAAATAAAATGACATGGATTAAAACCAATGGATAT
GGTGGAGCTATGGTTTGGACAGTCGACATGGATGACTTCACAGGAACTGTTTGTGGTAAT
AATGTAAAATATCCTTTGATTGGTGCCATGCGTGAAGAATTAAGAGGCGTTTCACGTGGT
AAAGATGCCAAGGATGTTGATTGGGAAAAAGTTGCTGCTTCATTTGATGATTTACCTGAA
AAACCAGAACCAATTAAAATTGATGTTGCTGAACTTTTGGCTAAAGTCAAAACAACACCA
GAAAAGAAAGTCAAAAAAATCAAACCTGGTGCAGCAACAATTGAAACTAAAAAAAGACCA
GCACAAATCTTTTGCTATCTTACAAGTTGGTCAATTAAAAGACCAGGTGCTGGAAAATTC
GAGCCTAAAGATGTCGATCCAAGTCTCTGTACTCATGTTGTTTATGCTTTTGCTACATTG
AAAGATCATAAATTAAGTGAAGCTAATGACAACGATCCAGATTTGTATGATCAAGTTTTG
GCTTTAAGAGAAAAAAATCCAGAATTGCAAGTTCTTTTGGCCATTGGTGGCTGGGCATTT
GGTTCAACACCATTTAAAGAATTAACTTCAAATGTCTTTAGAATGAACCAATTTGTTTAT
GAAGCTATTGAATTTTTAAGAGAATACAAATTCAATGGATTAGACATAAATTGGGAATAT
CCAAGAGGAGCCGAGGATCGAAAAGCTTATGTTAATCTTGTAAAAGAACTAAGAATTGCA
TTTGAAGGTGAAGCCAAAACATCAAGTGATCCAAGACTTCTTCTTACAGCTGCTGTACCA
GCTTCATTTGAAGCAATTGCAGCAGGATATGATGTGCCAGAAATTTCAAAATATCTTGAT
TTCATTAATGTTATGACTTATGATTTCCATGGTCAATGGGAGAGACAAGTTGGACACAAT
TCACCACTATTTCCATTAGAATCGGCTTCAGGTCATCAAAAGAAATTGACAGTTGATTTC
AGTGCACGTGAATGGGTCAAACAAGGAGCACCAAAAGAGAAATTATTAATTGGTATGCCA
ACTTATGGTCGTTCATTTACATTGATCAATGAGACACAATTTGATATTGGTGCACCTGCT
AGTGGTGGTGGATCACCTGGAAAATTCACAAATGAAGCTGGTTTTCTTGCTTATTATGAA
GTTTGCGGCTTTCTTTCACAACCAAATACAACTTTAGTGTGGGATTCAGAACAGCAAGTT
CCATTTGCTTACAAAAAAGATCAATGGGTTGGATTTGATGATGAAAGATCATTGAAAACA
AAGATGGAATGGCTGAAAGAAAATGGTTATGGTGGTGTAATGGTATATGCAATTGATATG
GATGATTTTAGCAACAGATGTGGAGCTGGAAAATATCCATTATTGACTACACTTAATACA
GAACTTGAAGGTTATAAAGTTGATTTAGAATATGATGGACCTTATGAATCTTATAATCCA
AATGGAAAATATACAACCAAAGATCCAAATGAAGTGACATGCGGTGAACAAGATGGCCAC
ATCAGTTATCATCCAGATAAGCTTGATTGTACACATTATTATATGTGTGAAGGTGAAAGA
AAACATCACATGCCTTGTCCAGTAAATTTAGTATTTAATCCGAATGAAAATGTTTGTGAT
TGGCCTGAGAATGTTGAAAGTTGTCAAAGCTACACCCAAGCGCCCAGCAGTTAA
>g8661.t1 Gene=g8661 Length=1017
MNEGYCMHPMLEHNLPRLFRLAFLACILLLIFSHLTDSAQEKRRIRRPTSLANKNTEKSS
SLRNATRSTSSILKKVAQDRVDLAPAPSTNRFKVRQRRPSTALAASASNDNSVSTSNEGI
KDKDGYKIVCYYTNWSQYRVKIGKFLPEDIPADLCTHIIFAFGWLKKGKLSSFESNDETK
DGKVGLYQRIVGLKKANPNLKVLLAIGGWSFGTQKFKEMSKSRYARQTFIYSAIPYLRQR
GFDGLDLDWEYPAGSDDKKNFVLLLKELRESFEAESQEIRQPRLLLSAAVPVGPDNIRGG
YDVPAVASYLDFINLMAYDFHGKWERETGHNAPLYAPSTDSEWRKQLSVDNAANLWVKMG
APKEKLIIGMPTYGRSFTLANTDKHGPNSPASGGGREGVYTKESGFLAYYEICEMLNNGA
VYVWDEEMKVPYMVDGDQWVGFDDERAIRNKMTWIKTNGYGGAMVWTVDMDDFTGTVCGN
NVKYPLIGAMREELRGVSRGKDAKDVDWEKVAASFDDLPEKPEPIKIDVAELLAKVKTTP
EKKVKKIKPGAATIETKKRPAQIFCYLTSWSIKRPGAGKFEPKDVDPSLCTHVVYAFATL
KDHKLSEANDNDPDLYDQVLALREKNPELQVLLAIGGWAFGSTPFKELTSNVFRMNQFVY
EAIEFLREYKFNGLDINWEYPRGAEDRKAYVNLVKELRIAFEGEAKTSSDPRLLLTAAVP
ASFEAIAAGYDVPEISKYLDFINVMTYDFHGQWERQVGHNSPLFPLESASGHQKKLTVDF
SAREWVKQGAPKEKLLIGMPTYGRSFTLINETQFDIGAPASGGGSPGKFTNEAGFLAYYE
VCGFLSQPNTTLVWDSEQQVPFAYKKDQWVGFDDERSLKTKMEWLKENGYGGVMVYAIDM
DDFSNRCGAGKYPLLTTLNTELEGYKVDLEYDGPYESYNPNGKYTTKDPNEVTCGEQDGH
ISYHPDKLDCTHYYMCEGERKHHMPCPVNLVFNPNENVCDWPENVESCQSYTQAPSS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 25 | g8661.t1 | CDD | cd02872 | GH18_chitolectin_chitotriosidase | 128 | 494 | 0.0 |
| 24 | g8661.t1 | CDD | cd02872 | GH18_chitolectin_chitotriosidase | 563 | 922 | 0.0 |
| 16 | g8661.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 128 | 493 | 1.8E-150 |
| 17 | g8661.t1 | Gene3D | G3DSA:3.10.50.10 | - | 372 | 442 | 1.8E-150 |
| 15 | g8661.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 564 | 921 | 7.8E-146 |
| 18 | g8661.t1 | Gene3D | G3DSA:3.10.50.10 | - | 801 | 872 | 7.8E-146 |
| 14 | g8661.t1 | Gene3D | G3DSA:2.170.140.10 | Antimicrobial Protein | 947 | 1015 | 5.3E-13 |
| 33 | g8661.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 42 | 68 | - |
| 31 | g8661.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 52 | 68 | - |
| 32 | g8661.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 87 | 116 | - |
| 34 | g8661.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 93 | 116 | - |
| 5 | g8661.t1 | PANTHER | PTHR11177 | CHITINASE | 123 | 520 | 1.6E-282 |
| 7 | g8661.t1 | PANTHER | PTHR11177:SF217 | OVIDUCT-SPECIFIC GLYCOPROTEIN | 123 | 520 | 1.6E-282 |
| 4 | g8661.t1 | PANTHER | PTHR11177 | CHITINASE | 560 | 938 | 1.6E-282 |
| 6 | g8661.t1 | PANTHER | PTHR11177:SF217 | OVIDUCT-SPECIFIC GLYCOPROTEIN | 560 | 938 | 1.6E-282 |
| 2 | g8661.t1 | Pfam | PF00704 | Glycosyl hydrolases family 18 | 127 | 471 | 9.5E-95 |
| 3 | g8661.t1 | Pfam | PF00704 | Glycosyl hydrolases family 18 | 563 | 901 | 9.4E-91 |
| 1 | g8661.t1 | Pfam | PF01607 | Chitin binding Peritrophin-A domain | 962 | 1008 | 4.6E-11 |
| 20 | g8661.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 38 | - |
| 21 | g8661.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 20 | - |
| 22 | g8661.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 21 | 32 | - |
| 23 | g8661.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 33 | 38 | - |
| 19 | g8661.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 39 | 1017 | - |
| 29 | g8661.t1 | ProSitePatterns | PS01095 | Chitinases family 18 active site. | 242 | 250 | - |
| 30 | g8661.t1 | ProSitePatterns | PS01095 | Chitinases family 18 active site. | 671 | 679 | - |
| 35 | g8661.t1 | ProSiteProfiles | PS50940 | Chitin-binding type-2 domain profile. | 951 | 1010 | 13.862 |
| 26 | g8661.t1 | SMART | SM00636 | 2g34 | 126 | 471 | 3.1E-153 |
| 27 | g8661.t1 | SMART | SM00636 | 2g34 | 561 | 901 | 7.5E-136 |
| 28 | g8661.t1 | SMART | SM00494 | chi_10 | 952 | 1010 | 5.9E-12 |
| 12 | g8661.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 126 | 495 | 8.22E-100 |
| 9 | g8661.t1 | SUPERFAMILY | SSF54556 | Chitinase insertion domain | 373 | 442 | 1.14E-25 |
| 11 | g8661.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 562 | 922 | 2.11E-95 |
| 8 | g8661.t1 | SUPERFAMILY | SSF54556 | Chitinase insertion domain | 802 | 872 | 1.05E-22 |
| 10 | g8661.t1 | SUPERFAMILY | SSF57625 | Invertebrate chitin-binding proteins | 947 | 1012 | 7.59E-15 |
| 13 | g8661.t1 | SignalP_EUK | SignalP-TM | SignalP-TM | 1 | 38 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0008061 | chitin binding | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0005576 | extracellular region | CC |
KEGG
Orthology
- This transcript has been annotated with K01183 (chitinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed