General information for g8749.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative phosphatidate phosphatase.
Parent gene
- The parent gene g8749 is located on chr_2: 32791246..32792993 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8749 | g8749.t1 | TTS | g8749.t1 | 32790268 | 32790268 |
| chr_2 | g8749 | g8749.t1 | isoform | g8749.t1 | 32790384 | 32791935 |
| chr_2 | g8749 | g8749.t1 | exon | g8749.t1.exon1 | 32790384 | 32790538 |
| chr_2 | g8749 | g8749.t1 | cds | g8749.t1.CDS1 | 32790384 | 32790538 |
| chr_2 | g8749 | g8749.t1 | exon | g8749.t1.exon2 | 32790599 | 32790928 |
| chr_2 | g8749 | g8749.t1 | cds | g8749.t1.CDS2 | 32790599 | 32790928 |
| chr_2 | g8749 | g8749.t1 | exon | g8749.t1.exon3 | 32791015 | 32791189 |
| chr_2 | g8749 | g8749.t1 | cds | g8749.t1.CDS3 | 32791015 | 32791189 |
| chr_2 | g8749 | g8749.t1 | exon | g8749.t1.exon4 | 32791246 | 32791397 |
| chr_2 | g8749 | g8749.t1 | cds | g8749.t1.CDS4 | 32791246 | 32791397 |
| chr_2 | g8749 | g8749.t1 | exon | g8749.t1.exon5 | 32791460 | 32791634 |
| chr_2 | g8749 | g8749.t1 | cds | g8749.t1.CDS5 | 32791460 | 32791634 |
| chr_2 | g8749 | g8749.t1 | exon | g8749.t1.exon6 | 32791903 | 32791935 |
| chr_2 | g8749 | g8749.t1 | cds | g8749.t1.CDS6 | 32791903 | 32791935 |
| chr_2 | g8749 | g8749.t1 | TSS | g8749.t1 | NA | NA |
Sequences
>g8749.t1 Gene=g8749 Length=1020
ATGCGACTCTTCTTTAAAAATAATCTTTTGTTTATGACAAACAGGCCCAATCGGTCAATA
CAAGCAATACAAGAAGAAGCGACTGAAAACAGCCCATTAAACAATCGACCTGTATCTACA
ATTGATAATTCAAACAAATTCACAATGGATGCTGAAACAAAACGAATATTGACAAAAATT
GCTATTGATGTAATTCTTTTAGGCTCTGTTGGCTTTCCTGTTCTGGCATATTTCCTCTTT
GGAGTTCCTTATGAACGTGGCTTTTTCTGTGATGATCAATCTTTGATGTATCCGTTTCAT
GATTCAACTGTAACAAGTGCAATGTTATATAGTTTTGGTTTTGGTATACCTACAATTTGC
ATTCTCATAATTGAATACTTAAGATGGAGGCTTAATATGGAACCTGAAAGAGAACTGAAA
CTTTTTGGTCGTTCAATCCCCGTTTGGGTTATAAATGCATACAGATTTATAGGCATTCTT
CTCTTTGGAGCTGCTTGCAGTCAATTGATAACTGATATTGCAAAATATACAATTGGTCGA
TTACGTCCTCATTTCTTAAGTGTTTGCCAACCGATTATGCCTGATGGAACTAATTGCTCG
GATATAATAAATCACAACAAATACATCATTGATTTCACATGCAGTAATGAAAATGCTTCA
AAAAGGAAGTTAAAAGAAATGAGATTGAGTTTTCTAAGTGGACATAGTTCATTTAGTATG
TACACGATGGTCTATGCTGCTCTTTATATTCATAGCCGCATGGAATGGAAAGGATCAAAG
TTATTTAAACATTTTCTTCAATTTATATTTATTGCATTGGCATGGTATACAGCATTATCA
AGAGTCAGCAATTATAAGCATCACTGGTCCGATGTGATGGCGGGTTCCATTCAAGGTCTA
TTTGTTTCATTGTTAATCGTCTTCGGTGTTTCTGGTCTATTCAAAAATAAATTCAAAATT
GTTGAACAACCAAAAGGATCACGTTATGAGTTAAACTCGCATAGTACCAGAAGCAATTAG
>g8749.t1 Gene=g8749 Length=339
MRLFFKNNLLFMTNRPNRSIQAIQEEATENSPLNNRPVSTIDNSNKFTMDAETKRILTKI
AIDVILLGSVGFPVLAYFLFGVPYERGFFCDDQSLMYPFHDSTVTSAMLYSFGFGIPTIC
ILIIEYLRWRLNMEPERELKLFGRSIPVWVINAYRFIGILLFGAACSQLITDIAKYTIGR
LRPHFLSVCQPIMPDGTNCSDIINHNKYIIDFTCSNENASKRKLKEMRLSFLSGHSSFSM
YTMVYAALYIHSRMEWKGSKLFKHFLQFIFIALAWYTALSRVSNYKHHWSDVMAGSIQGL
FVSLLIVFGVSGLFKNKFKIVEQPKGSRYELNSHSTRSN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g8749.t1 | CDD | cd03384 | PAP2_wunen | 153 | 307 | 1.5489E-69 |
| 5 | g8749.t1 | Gene3D | G3DSA:1.20.144.10 | - | 64 | 337 | 8.9E-32 |
| 2 | g8749.t1 | PANTHER | PTHR10165 | LIPID PHOSPHATE PHOSPHATASE | 55 | 316 | 1.2E-94 |
| 3 | g8749.t1 | PANTHER | PTHR10165:SF173 | FI04477P-RELATED | 55 | 316 | 1.2E-94 |
| 1 | g8749.t1 | Pfam | PF01569 | PAP2 superfamily | 159 | 308 | 1.0E-25 |
| 9 | g8749.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 63 | - |
| 15 | g8749.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 64 | 84 | - |
| 12 | g8749.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 85 | 103 | - |
| 14 | g8749.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 104 | 127 | - |
| 8 | g8749.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 128 | 147 | - |
| 18 | g8749.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 148 | 170 | - |
| 11 | g8749.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 171 | 228 | - |
| 13 | g8749.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 229 | 249 | - |
| 6 | g8749.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 250 | 260 | - |
| 16 | g8749.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 261 | 280 | - |
| 10 | g8749.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 281 | 291 | - |
| 17 | g8749.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 292 | 314 | - |
| 7 | g8749.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 315 | 339 | - |
| 25 | g8749.t1 | SMART | SM00014 | acid_phosph_2 | 157 | 307 | 9.9E-38 |
| 4 | g8749.t1 | SUPERFAMILY | SSF48317 | Acid phosphatase/Vanadium-dependent haloperoxidase | 60 | 307 | 4.19E-26 |
| 23 | g8749.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 60 | 82 | - |
| 20 | g8749.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 102 | 124 | - |
| 21 | g8749.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 229 | 251 | - |
| 22 | g8749.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 266 | 285 | - |
| 24 | g8749.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 292 | 314 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0042577 | lipid phosphatase activity | MF |
| GO:0006644 | phospholipid metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01080 (phosphatidate phosphatase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
