General information for g877.t15
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 2-hydroxyacylsphingosine 1-beta-galactosyltransferase.
Parent gene
- The parent gene g877 is located on chr_3: 6619998..6629641 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g877 | g877.t15 | TSS | g877.t15 | 6618107 | 6618107 |
| chr_3 | g877 | g877.t15 | isoform | g877.t15 | 6618133 | 6620603 |
| chr_3 | g877 | g877.t15 | exon | g877.t15.exon1 | 6618133 | 6618367 |
| chr_3 | g877 | g877.t15 | cds | g877.t15.CDS1 | 6618133 | 6618367 |
| chr_3 | g877 | g877.t15 | exon | g877.t15.exon2 | 6618532 | 6618718 |
| chr_3 | g877 | g877.t15 | cds | g877.t15.CDS2 | 6618532 | 6618718 |
| chr_3 | g877 | g877.t15 | exon | g877.t15.exon3 | 6619039 | 6619626 |
| chr_3 | g877 | g877.t15 | cds | g877.t15.CDS3 | 6619039 | 6619626 |
| chr_3 | g877 | g877.t15 | exon | g877.t15.exon4 | 6619684 | 6620603 |
| chr_3 | g877 | g877.t15 | cds | g877.t15.CDS4 | 6619684 | 6620155 |
| chr_3 | g877 | g877.t15 | TTS | g877.t15 | 6620992 | 6620992 |
Sequences
>g877.t15 Gene=g877 Length=1930
ATGAAGCTCTCTTTAGCATTAATTGTTGTTTTTATCAATGGAATATTTGGTTTTAAAGTG
TTGGGAATTTTGCCTTTTGGCAGCAAGTCACATTTTGCAATTGGACATGCAATTTTGAAA
AGTTTAGCAGAAGCTGGACATAATGTAACGTCGATTTCACCATATCCATTAAAAGAACCT
ATGGAAAATTACAAAGATATCAGCACAGAAGATTACGTTGAAGTATTTTTTAAAATTGTG
AAATATCATGCAGTTCATCCTAAAGTTATTGAATTTATGAACACTTATGAGAAGTTTGAT
ATTTGCTTTATGGAAATTTTTAATTATGATGCATTACTTGGAATTGCCGAACATGTTGGT
TGCAAAGTTATTTCGTATACCACAACAGCTGTTGTTAAATGGGCTGATGATATGACTGGA
AACGTTTCACCACCATCATATGTACCAAGACCGTATGTTCAATATTCAGATAAAATGTCA
TTCAGAGAACGACTTATGAATACATTTTACGCTCACATTGAAGACATAGTTTATGAATTC
ATCATAAAACCAAACCAAAGAAGACTTTATGAGAAATATTTCCCAAATGCAACTAAAACA
TTTGACGAGATGTACAAAGATACTTCAATGATTTTCATGAACACTCACATTTCACTGACA
GCACCTCGTCCGTATTTACCAAGTTTTGTAGAAATTGGAGGAATTCATATTCAACCTGTA
AAAAAGTTGCCAAAAGTATTTCAAAATTTCTTAGATTCAGCGACTGATGGTGTGATTTTA
TTTTCAATGGGTTCAGTAATTCGATCATACCAATGGCCAGTTGAGAAACGTGAAGCATTC
GTTAAAGTTTTTGGCAAATTGAAGCAAAAAGTACTTTGGAAATATGAAAATGACACATTA
CCAAATAAACCTGATAATGTTATGATTAGTTCATGGTTGCCACAAAGAGACATTTTGGCT
CATCCTAATGTAAAAGCTTATATCTGTCATGGCGGACTATTAGGAACAACTGAAGCAGTT
TATGAAGGAGTCCCTGTGCTTGGTATACCAATATTCGGAGACCAAAAGACAATCATGGCT
AAAGCAGTTACTCGTGGCTATGGATTGCAAGTTTATTTCAAAGATGTTTCTGAAGAAACA
ATTGGTTCTGCCTTGGATGAATTATTGAACAATCCAATGTACAAAGAAAATGCAAAAATT
ATTTCAACACGCTTTAAAGATCGACCAATGACACCACAACAATCAGTAGTTTATTGGACT
GAATATGTTCATAGACACGGTGGAGCACCACACTTGAAAGCTGCTGGAAATGATTTGGAT
TTTATCGCATTTCATATGATTGATGTTTATGCTGTTTTGTTTTTTGGTGGAATTTCTTTC
ATTTTCATTAATTATTTGATTATTAAGTGGATTTTTATGAAATTTTCTGGAAAACCAAAG
AAGACAGAAAAGAAAAATGAGAAGAAGCTTAAAAAGAAATAAACAAGAAATAATAGAATA
TTTCAACCATTAGCTGTGACCAGTAAAATTTCAATCAGGGAACTGAGTTCTGTTCATATC
GAATGAAAAATATTTTTGAACAAATTTTAAAAGAAATCTCTGAATTCATTTATTTATTTA
CATTTAAGATTATGGCTTAATATTTTGCAACAATTAAAAACTTAGTTCATTAAAAAGTGT
TCCTTACATTTTTCATTATTTTATATTTTATTATTTCTTGAAAAATTATTTAAGTTCAAA
ATAATTTCCTTAAAATTCCATGTAAAGCTATAAACAGTGATATTTTCTTTTACTTTTGCT
CAATTAAATTTTATTATTTATTTTCTAGCTATATTTTCATTTTAATTAAAGTTCATTTTC
CTAAAAAAATTTTTGGCAAAAGAAATCATGAAATTTTCAAAAAACATGAAAGAATGAGAG
TGATTGTAAA
>g877.t15 Gene=g877 Length=493
MKLSLALIVVFINGIFGFKVLGILPFGSKSHFAIGHAILKSLAEAGHNVTSISPYPLKEP
MENYKDISTEDYVEVFFKIVKYHAVHPKVIEFMNTYEKFDICFMEIFNYDALLGIAEHVG
CKVISYTTTAVVKWADDMTGNVSPPSYVPRPYVQYSDKMSFRERLMNTFYAHIEDIVYEF
IIKPNQRRLYEKYFPNATKTFDEMYKDTSMIFMNTHISLTAPRPYLPSFVEIGGIHIQPV
KKLPKVFQNFLDSATDGVILFSMGSVIRSYQWPVEKREAFVKVFGKLKQKVLWKYENDTL
PNKPDNVMISSWLPQRDILAHPNVKAYICHGGLLGTTEAVYEGVPVLGIPIFGDQKTIMA
KAVTRGYGLQVYFKDVSEETIGSALDELLNNPMYKENAKIISTRFKDRPMTPQQSVVYWT
EYVHRHGGAPHLKAAGNDLDFIAFHMIDVYAVLFFGGISFIFINYLIIKWIFMKFSGKPK
KTEKKNEKKLKKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g877.t15 | CDD | cd03784 | GT1_Gtf-like | 19 | 419 | 2.089E-61 |
| 7 | g877.t15 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 238 | 407 | 5.6E-44 |
| 2 | g877.t15 | PANTHER | PTHR48043 | EG:EG0003.4 PROTEIN-RELATED | 17 | 88 | 7.0E-129 |
| 4 | g877.t15 | PANTHER | PTHR48043:SF114 | DOROTHY, ISOFORM A-RELATED | 17 | 88 | 7.0E-129 |
| 3 | g877.t15 | PANTHER | PTHR48043 | EG:EG0003.4 PROTEIN-RELATED | 93 | 457 | 7.0E-129 |
| 5 | g877.t15 | PANTHER | PTHR48043:SF114 | DOROTHY, ISOFORM A-RELATED | 93 | 457 | 7.0E-129 |
| 1 | g877.t15 | Pfam | PF00201 | UDP-glucoronosyl and UDP-glucosyl transferase | 71 | 481 | 1.4E-83 |
| 10 | g877.t15 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 11 | g877.t15 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 12 | g877.t15 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 12 | - |
| 14 | g877.t15 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 9 | g877.t15 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 448 | - |
| 13 | g877.t15 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 449 | 472 | - |
| 8 | g877.t15 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 473 | 493 | - |
| 18 | g877.t15 | ProSitePatterns | PS00375 | UDP-glycosyltransferases signature. | 312 | 355 | - |
| 6 | g877.t15 | SUPERFAMILY | SSF53756 | UDP-Glycosyltransferase/glycogen phosphorylase | 20 | 432 | 1.54E-103 |
| 17 | g877.t15 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 27 | - |
| 16 | g877.t15 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 451 | 473 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008194 | UDP-glycosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04628 (ceramide galactosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
