General information for g8816.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Guanine nucleotide-binding protein G(s) subunit alpha.
Parent gene
- The parent gene g8816 is located on chr_2: 33320511..33322702 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8816 | g8816.t1 | isoform | g8816.t1 | 33320070 | 33322702 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon1 | 33320070 | 33320186 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS1 | 33320070 | 33320186 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon2 | 33320266 | 33320427 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS2 | 33320266 | 33320427 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon3 | 33320517 | 33320613 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS3 | 33320517 | 33320613 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon4 | 33320738 | 33320858 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS4 | 33320738 | 33320858 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon5 | 33320932 | 33321060 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS5 | 33320932 | 33321060 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon6 | 33321133 | 33321312 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS6 | 33321133 | 33321312 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon7 | 33321375 | 33321499 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS7 | 33321375 | 33321499 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon8 | 33322361 | 33322434 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS8 | 33322361 | 33322434 |
| chr_2 | g8816 | g8816.t1 | exon | g8816.t1.exon9 | 33322556 | 33322702 |
| chr_2 | g8816 | g8816.t1 | cds | g8816.t1.CDS9 | 33322556 | 33322702 |
| chr_2 | g8816 | g8816.t1 | TTS | g8816.t1 | 33322835 | 33322835 |
| chr_2 | g8816 | g8816.t1 | TSS | g8816.t1 | NA | NA |
Sequences
>g8816.t1 Gene=g8816 Length=1152
ATGGGTTGTTTTGGCTCAGCTAGTTCCAAACAACAGGCAGATTCAAGTTCGGATGATTCT
AAAAGTCAAAAACGACGAAGCGATGCGATTACAAAGCAACTACAAAAGGATAAGCAGGTG
TACAGAGCAACGCATAGATTGCTCTTACTCGGTGCTGGTGAATCGGGTAAATCGACGATC
GTTAAACAAATGCGTATATTGCACGTGAATGGTTTCTCAGATACCGAACGCAAACAAAAG
ATTGAAGACATCAAGAAAAACATACGCGATGCAATATTGACTATTACGGGCGCCATGTCC
ACTCTTACACCTCCAATATCACTAGAGAAACCAGAGAATCAAGCGAGAGTAGATTATATT
CAGGATTATGCTTCAAATCCTGATTTCAATTATCCACCTGAATTTTATGAGCACACGGAA
GAACTATGGAAAGACGCGGGTGTTCAACAGACTTTTGAGCGATCAAATGAGTATCAGCTG
ATTGATTGTGCGAAATACTTCTTGGACCGAGTTAGTGTAGTAAAGCAACCAAATTATACA
CCATCGGAACAAGATATTTTAAGATGCCGTGTCCTCACATCGGGAATTTTCGAGACCAGA
TTTCAAGTGGATAAAGTTAATTTTCACATGTTCGATGTGGGTGGTCAAAGAGACGAACGT
CGAAAATGGATACAATGTTTCAATGATGTTACTGCAATTATTTTTGTGACTGCATGTTCT
AGCTATAACATGGTGTTGCGCGAAGATCCAACACAAAATAGATTAAGAGAATCATTGGAC
TTATTTAAAAGCATTTGGAATAATAGATGGCTTCGAACTATCTCAGTTATTCTTTTTCTA
AACAAACAGGACTTATTAGCTGAGAAAGTAAAAGCACAAAAAAGTAAACTGTCTGATTAT
TTTGCTGAATTTAATAGGTATCAAACACCAGCGGATGCATTGATAGAACCAGGAGACGAT
CCAGAAGTAATTAGAGCCAAATATTTTATTAGAGATGAATTTCTGCGCATTTCAACAGCG
AGTGGAGACGGGAAACATTATTGTTATCCACATTTTACATGTGCAGTTGATACTGAAAAT
ATTAAGAGAGTGTTCAATGATTGTCGAGACATTATTCAGCGAATGCATTTACGCCAATAT
GAACTTTTATAA
>g8816.t1 Gene=g8816 Length=383
MGCFGSASSKQQADSSSDDSKSQKRRSDAITKQLQKDKQVYRATHRLLLLGAGESGKSTI
VKQMRILHVNGFSDTERKQKIEDIKKNIRDAILTITGAMSTLTPPISLEKPENQARVDYI
QDYASNPDFNYPPEFYEHTEELWKDAGVQQTFERSNEYQLIDCAKYFLDRVSVVKQPNYT
PSEQDILRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVTACS
SYNMVLREDPTQNRLRESLDLFKSIWNNRWLRTISVILFLNKQDLLAEKVKAQKSKLSDY
FAEFNRYQTPADALIEPGDDPEVIRAKYFIRDEFLRISTASGDGKHYCYPHFTCAVDTEN
IKRVFNDCRDIIQRMHLRQYELL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g8816.t1 | CDD | cd00066 | G-alpha | 45 | 377 | 0.0 |
| 17 | g8816.t1 | Gene3D | G3DSA:3.40.50.300 | - | 46 | 372 | 6.6E-148 |
| 18 | g8816.t1 | Gene3D | G3DSA:1.10.400.10 | GI Alpha 1 | 72 | 192 | 6.6E-148 |
| 21 | g8816.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 16 | - |
| 22 | g8816.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 34 | - |
| 23 | g8816.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 17 | 34 | - |
| 2 | g8816.t1 | PANTHER | PTHR10218:SF233 | GUANINE NUCLEOTIDE-BINDING PROTEIN G(OLF) SUBUNIT ALPHA | 14 | 381 | 2.3E-189 |
| 3 | g8816.t1 | PANTHER | PTHR10218 | GTP-BINDING PROTEIN ALPHA SUBUNIT | 14 | 381 | 2.3E-189 |
| 13 | g8816.t1 | PRINTS | PR00318 | Alpha G protein (transducin) signature | 46 | 61 | 1.9E-46 |
| 8 | g8816.t1 | PRINTS | PR00443 | G protein alpha subunit group S signature | 75 | 89 | 5.2E-45 |
| 7 | g8816.t1 | PRINTS | PR00443 | G protein alpha subunit group S signature | 90 | 105 | 5.2E-45 |
| 6 | g8816.t1 | PRINTS | PR00443 | G protein alpha subunit group S signature | 106 | 120 | 5.2E-45 |
| 12 | g8816.t1 | PRINTS | PR00318 | Alpha G protein (transducin) signature | 179 | 201 | 1.9E-46 |
| 11 | g8816.t1 | PRINTS | PR00318 | Alpha G protein (transducin) signature | 208 | 225 | 1.9E-46 |
| 14 | g8816.t1 | PRINTS | PR00318 | Alpha G protein (transducin) signature | 230 | 258 | 1.9E-46 |
| 9 | g8816.t1 | PRINTS | PR00443 | G protein alpha subunit group S signature | 259 | 274 | 5.2E-45 |
| 10 | g8816.t1 | PRINTS | PR00318 | Alpha G protein (transducin) signature | 276 | 285 | 1.9E-46 |
| 5 | g8816.t1 | PRINTS | PR00443 | G protein alpha subunit group S signature | 323 | 337 | 5.2E-45 |
| 4 | g8816.t1 | PRINTS | PR00443 | G protein alpha subunit group S signature | 338 | 351 | 5.2E-45 |
| 1 | g8816.t1 | Pfam | PF00503 | G-protein alpha subunit | 24 | 372 | 1.5E-109 |
| 24 | g8816.t1 | ProSiteProfiles | PS51882 | G-alpha domain profile. | 43 | 383 | 96.04 |
| 20 | g8816.t1 | SMART | SM00275 | galpha_1 | 24 | 382 | 1.5E-182 |
| 15 | g8816.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 42 | 381 | 2.8E-62 |
| 16 | g8816.t1 | SUPERFAMILY | SSF47895 | Transducin (alpha subunit), insertion domain | 72 | 193 | 1.7E-37 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0031683 | G-protein beta/gamma-subunit complex binding | MF |
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0007165 | signal transduction | BP |
| GO:0019001 | guanyl nucleotide binding | MF |
| GO:0005525 | GTP binding | MF |
| GO:0003924 | GTPase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04632 (guanine nucleotide-binding protein G(s) subunit alpha)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
