General information for g8824.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Homeodomain-interacting protein kinase 2.
Parent gene
- The parent gene g8824 is located on chr_2: 33369609..33373460 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8824 | g8824.t1 | TTS | g8824.t1 | 33368609 | 33368609 |
| chr_2 | g8824 | g8824.t1 | isoform | g8824.t1 | 33369609 | 33373460 |
| chr_2 | g8824 | g8824.t1 | exon | g8824.t1.exon1 | 33369609 | 33369731 |
| chr_2 | g8824 | g8824.t1 | cds | g8824.t1.CDS1 | 33369609 | 33369731 |
| chr_2 | g8824 | g8824.t1 | exon | g8824.t1.exon2 | 33369791 | 33370464 |
| chr_2 | g8824 | g8824.t1 | cds | g8824.t1.CDS2 | 33369791 | 33370464 |
| chr_2 | g8824 | g8824.t1 | exon | g8824.t1.exon3 | 33370527 | 33372280 |
| chr_2 | g8824 | g8824.t1 | cds | g8824.t1.CDS3 | 33370527 | 33372280 |
| chr_2 | g8824 | g8824.t1 | exon | g8824.t1.exon4 | 33372337 | 33372546 |
| chr_2 | g8824 | g8824.t1 | cds | g8824.t1.CDS4 | 33372337 | 33372546 |
| chr_2 | g8824 | g8824.t1 | exon | g8824.t1.exon5 | 33372618 | 33372718 |
| chr_2 | g8824 | g8824.t1 | cds | g8824.t1.CDS5 | 33372618 | 33372718 |
| chr_2 | g8824 | g8824.t1 | exon | g8824.t1.exon6 | 33373374 | 33373460 |
| chr_2 | g8824 | g8824.t1 | cds | g8824.t1.CDS6 | 33373374 | 33373460 |
| chr_2 | g8824 | g8824.t1 | TSS | g8824.t1 | NA | NA |
Sequences
>g8824.t1 Gene=g8824 Length=2949
ATGCTAGAACAGAACTTGTATGATTTCCTGAAACAAAACAAATTTTCGCCATTACCATTG
AAGTACATCAGACCGATTTTGCAACAGGTTTTAACAGCGTTGCTAAAGCTGAAGCAATTG
GGTTTGATTCATGCGGATTTGAAGCCTGAGAACATCATGCTTGTAGATCCCGTTCGTCAA
CCATACAGGGTAAAAGTGATTGATTTTGGAAGTGCATCTCATGTAAGCAAAACAGTCTGC
AATACATACCTTCAATCACGATATTATCGTGCACCAGAAATCATTCTTGGCTTGCCTTTT
TGCGAAGCAATTGATATGTGGTCACTTGGATGTGTCGTTGCTGAGCTTTTCTTGGGATGG
CCTCTCTATCCAGGCTCTTCTGAGTATGATCAAATCAGATACATATCACAAACACAAGGT
CTACCTACAGAGCATATGCTAAATAGTGCGAGCAAGACAGAAAAGTTTTTCTATCGTGAC
GAAGATTCTACATATCCCTTCTGGCGATTAATTTCACCTGAAGAGAATGAAATTATGACC
AATGTTAAGAGCAAGGAGGCTCGCAAATATATTTTCAATTGTCTCGACGATATCGGTCAA
GTGAATGTGCAAATGGACATAGAAAGCAGTCAATTGTTGGCAGAAAAAATCGATAGAAGA
GAATTCATTGATTTACTGAAACGAATGCTGACAATTGATCAAGAACGTCGCATTCAGCCA
GCGCAAGCTTTAAGACATCCATTTGTAACACTTTCACATTTGGTTGATTATGCCCATTGT
AATAATGTTAAAGCAAGTGTTCAAATGATGGAAGTTTGTAGACGAGATCCAGTCATACAT
TCTGTTCCACAGACCACTGCCACATTGGTCACAAACTTTGGACCAAATACAACAGAAAAT
ATGACATTCACCATTAATAATCAGCTTAATCAAGTTCAAAGATTAGTGCGTGAAAGAAAT
CCTTCGTCATATGAAAATGTCTATCAGTTTTATGGTACACCAAGAAATGTTGTTCGACAG
TATGCAAATACTAGAGCGGCTGAAGTTTTACCGCCTCAACTGAGTTTCATCTGTCCTTAT
AATCCCATGCCTAGTCCTACTACAACTAAACACGTTGTCGTAGGAAGCGCAGGAATGCAA
CCATCATTGCAAGTTCCACCGCAACAGTACGTAAATGTTCCTGTGCCCGTTTCTATGACA
ATGGAACCAAATGGTCAACGAATGCTTCTCACAAATGCTGCTGTTCAATCAAGTGTTGCT
TGGCCACAAGGAAGTACTCGACAAGTTGCCATTGTACCCAATTGGAGTCAACATGGTACA
GCACCGCATTCGCTAATTGTTGATTCACAATTTTACAATGTGGAAGAAATTTATGGCAAG
CAGCCGTTGAGTATTCACAAGTATGAAAAGAAGGAATCACCTGTGCATCATTTGAATGTA
CCGCGTCATGATAAGAAAGAAACCAATCAGTTATCTCCAGTGAAGAAACGTGTAAAAGAA
AATACACCGCCTAATCACCATCATCATCATCAGAATCCTAATCTTCAACAAAATCAGACA
AGATACAATAATAACAACCATAGGTCATCATCATATCATCACCATGTATCACCACAGCAA
ACTAATTCATCAATGAACCAATATCATCCTAATCAAGCTTTCTATGGAAGTCAGGGTCAA
CAGCTCCCAACTGGTTATTATGATCAAAATCAAGTTTATTCATCGGCTGGTGTTATTAAT
ACAAGCGGCAAACAAAGAAGAGCAAGAAATCAAACTTCTACAGCCACTCATCATCAGATT
GTAAATTCAACTATCACTATCCATGATACACCATCACCTCCTCCACAAATCACACAAACT
CCTTCTGTTATTTTAATCTCAGACAGTGAAGATGAGGAAGAGAATCAGAAAAAGCAACAG
CAGCAGCAGTCAACAAAGAGTTCTCGTGAGAAAACAATCGTTGGTACAAATAACAATCAA
AGTTATTCAAATACAACAAATTCATCAACGCAATGCAACAAAAATGATTCACAGAACTCG
ACTGTCATAAGCAGCAATAGTAATCAGCAGAGAAAACAAGTCATCACAGTGGGTGATAGT
GATGGAGAAGACACAAGTCCAAAAGGCCAACAGCAGCAGCAACATAGTAATGTAAAATAT
GAACAACAACATGCAACACAAAAGAAACGCTTATTGGCGATGACACAGAATGATCCAAAT
ATCACAAACAATTCCTCGTCTTCGACAAATCAATTGAAGCAAGAACCTGCAGATTTCAAT
ACATCATCAAATTCGTTAAATTACGAATATCCACCATTTGATCATCAAAAACGGTCATCA
TGGGTTGGTCCATCATCGACAGCCGTATCAACAATAGTTCAACCATCGAACATTCATCAG
AATCATCATGTATCGCATAATAATAAACGTGATACATCTGGCTGCGGATCAAGTTCGAGC
TCACAACAACAACAACAGCAGCAGCAGCCAATAGTGGCACATGGCAAGAGTGATATGCCA
TTATCGTCAAGCTCTAATACTACCTCGTTAAACAGTACACCAATATTTCACCACCATCAT
CATCATAATAATTCGAGTCATCATCATCGCACGCAGCCTCAAAATATTACGCCTTTGGGT
AATTCACCCATTAGCGGAGGATCGCAAGCTACGGCTTTGTTGCAGCAGACACAGCAACCT
GATATTTATGCCCAAGCAAATGAAATTTACAGAAACAGGTCAACTCTCTATGTGCAACAA
CCGACATATGCTTACGACACTCGCATAATTCAGCCACCGCCAGCACATAATCCATCAAAT
CGACAGGTGATGCCAACACATCAATTGCCTGCACATATTAATTTTCCATCATATGGTCAA
TTTGGTGCATCACCTTTAAGTCCTCAAGTGGCAGCAACATTGAGACCCAACTTTTGGCTT
GCTGAGTAA
>g8824.t1 Gene=g8824 Length=982
MLEQNLYDFLKQNKFSPLPLKYIRPILQQVLTALLKLKQLGLIHADLKPENIMLVDPVRQ
PYRVKVIDFGSASHVSKTVCNTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVVAELFLGW
PLYPGSSEYDQIRYISQTQGLPTEHMLNSASKTEKFFYRDEDSTYPFWRLISPEENEIMT
NVKSKEARKYIFNCLDDIGQVNVQMDIESSQLLAEKIDRREFIDLLKRMLTIDQERRIQP
AQALRHPFVTLSHLVDYAHCNNVKASVQMMEVCRRDPVIHSVPQTTATLVTNFGPNTTEN
MTFTINNQLNQVQRLVRERNPSSYENVYQFYGTPRNVVRQYANTRAAEVLPPQLSFICPY
NPMPSPTTTKHVVVGSAGMQPSLQVPPQQYVNVPVPVSMTMEPNGQRMLLTNAAVQSSVA
WPQGSTRQVAIVPNWSQHGTAPHSLIVDSQFYNVEEIYGKQPLSIHKYEKKESPVHHLNV
PRHDKKETNQLSPVKKRVKENTPPNHHHHHQNPNLQQNQTRYNNNNHRSSSYHHHVSPQQ
TNSSMNQYHPNQAFYGSQGQQLPTGYYDQNQVYSSAGVINTSGKQRRARNQTSTATHHQI
VNSTITIHDTPSPPPQITQTPSVILISDSEDEEENQKKQQQQQSTKSSREKTIVGTNNNQ
SYSNTTNSSTQCNKNDSQNSTVISSNSNQQRKQVITVGDSDGEDTSPKGQQQQQHSNVKY
EQQHATQKKRLLAMTQNDPNITNNSSSSTNQLKQEPADFNTSSNSLNYEYPPFDHQKRSS
WVGPSSTAVSTIVQPSNIHQNHHVSHNNKRDTSGCGSSSSSQQQQQQQQPIVAHGKSDMP
LSSSSNTTSLNSTPIFHHHHHHNNSSHHHRTQPQNITPLGNSPISGGSQATALLQQTQQP
DIYAQANEIYRNRSTLYVQQPTYAYDTRIIQPPPAHNPSNRQVMPTHQLPAHINFPSYGQ
FGASPLSPQVAATLRPNFWLAE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g8824.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1 | 253 | 4.8E-65 |
| 8 | g8824.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 472 | 495 | - |
| 14 | g8824.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 472 | 494 | - |
| 9 | g8824.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 580 | 728 | - |
| 11 | g8824.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 580 | 626 | - |
| 10 | g8824.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 640 | 721 | - |
| 13 | g8824.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 740 | 760 | - |
| 15 | g8824.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 795 | 847 | - |
| 12 | g8824.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 810 | 847 | - |
| 2 | g8824.t1 | PANTHER | PTHR24058 | DUAL SPECIFICITY PROTEIN KINASE | 1 | 692 | 2.1E-189 |
| 3 | g8824.t1 | PANTHER | PTHR24058:SF43 | HOMEODOMAIN-INTERACTING PROTEIN KINASE 1 | 1 | 692 | 2.1E-189 |
| 1 | g8824.t1 | Pfam | PF00069 | Protein kinase domain | 4 | 249 | 2.0E-33 |
| 6 | g8824.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 42 | 54 | - |
| 16 | g8824.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 249 | 26.591 |
| 7 | g8824.t1 | SMART | SM00220 | serkin_6 | 1 | 249 | 5.7E-19 |
| 4 | g8824.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 3 | 251 | 1.46E-50 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K08826 (homeodomain interacting protein kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
