Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable multidrug resistance-associated protein lethal(2)03659.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8918 g8918.t6 TSS g8918.t6 33804011 33804011
chr_2 g8918 g8918.t6 isoform g8918.t6 33804724 33805909
chr_2 g8918 g8918.t6 exon g8918.t6.exon1 33804724 33805539
chr_2 g8918 g8918.t6 cds g8918.t6.CDS1 33805032 33805539
chr_2 g8918 g8918.t6 exon g8918.t6.exon2 33805600 33805679
chr_2 g8918 g8918.t6 cds g8918.t6.CDS2 33805600 33805679
chr_2 g8918 g8918.t6 exon g8918.t6.exon3 33805742 33805909
chr_2 g8918 g8918.t6 cds g8918.t6.CDS3 33805742 33805909
chr_2 g8918 g8918.t6 TTS g8918.t6 33806221 33806221

Sequences

>g8918.t6 Gene=g8918 Length=1064
ATGGAGTCAAATTCAAAACAACAAAATTTACCAAAACATCCAAGAGAAAATGGAAGCGTG
TTTTCATATTTAACATTTAGGTATGAAAGTTCTCACATGTCAATTATTTAATGAAATAAT
AAAAAAATTTCTTTTAATTCAGTTGGGTATTGCCGTTGTTGCTAAAAGGAAGAAAGAAAA
CTTTAGAATTAGAGGACTTGTATCAACCTCTTTCACAACATTTGGCAGCAAATTTAGGAA
CAAAATTGGAAAAATCATGGAAAAATGAAGTAAATTCAAAGCGATCAAAAAATAAAAATC
CAAGTTTGATGCGTGCGGGCTTAAAAGTTTTTGGTCCTAATTTAATGTCACATGGATTTT
TTCTTTTATTAATTGAAATGTGTTTCAAAGTCACAATGCCCCTTCTGCTTGGTGGTATAT
TGCGGTATTATGGAAATCCAAACAATTCGGATCCTAAAATTGCTTATTTTTATGCTGGCG
GGTTGATTTTGTGCAGCTTTCTTAATGTCATTTGTGCACATGGTTTGATGCTGAGTAATT
TGAATGTTGGAATGAAGATGCGAGTTTCAGCTTGTTCATTGATATATCGAAAATCTTTAC
GTCTCAGTAAATCAGCATTACTTAATACAACGAGTGGTCAAGTTGTTAATTTGCTTTCTA
ATGATGTTGGACGATTCGAACTTATTGCTATGTTTGTGCATTATTTATGGGTTGGTCCAA
TTGAAACAATTGTGATAGGAGTATTGATGTATATGGAAATTGGAATATCTGCTATAGCTG
GTATTCTCTTCTTATTGCTCTTTATTCCATTTCAATTCTATCTTGGAAAAAAAACATCGC
AACTTCGTTTAAGAGTAGCTCTTAGGACTGATGAGAGAGTAAGGTTGATGAATGAGATCA
TTCAAGGTATACAAGTTATTAAAATGTACGCTTGGGAAAAACCTTTCGCCAAAGTCGTCT
CGATGGCTCGTGGTAGAGAAATTCGTGCAATTAGATTTGCATCATGGATACGTGGAATTT
TACTCTCATTCATTATGTTCAGTTCCCGTGTGTCAATTTTTACA

>g8918.t6 Gene=g8918 Length=252
MRAGLKVFGPNLMSHGFFLLLIEMCFKVTMPLLLGGILRYYGNPNNSDPKIAYFYAGGLI
LCSFLNVICAHGLMLSNLNVGMKMRVSACSLIYRKSLRLSKSALLNTTSGQVVNLLSNDV
GRFELIAMFVHYLWVGPIETIVIGVLMYMEIGISAIAGILFLLLFIPFQFYLGKKTSQLR
LRVALRTDERVRLMNEIIQGIQVIKMYAWEKPFAKVVSMARGREIRAIRFASWIRGILLS
FIMFSSRVSIFT

Protein features from InterProScan

Transcript Database ID Name Start End E.value
10 g8918.t6 Gene3D G3DSA:1.20.1560.10 - 11 252 1.4E-17
2 g8918.t6 PANTHER PTHR24223:SF360 MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659-LIKE PROTEIN-RELATED 3 251 3.9E-76
3 g8918.t6 PANTHER PTHR24223 ATP-BINDING CASSETTE SUB-FAMILY C 3 251 3.9E-76
1 g8918.t6 Pfam PF00664 ABC transporter transmembrane region 16 250 1.7E-28
12 g8918.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 11 -
20 g8918.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 12 41 -
17 g8918.t6 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 42 52 -
22 g8918.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 53 75 -
14 g8918.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 76 124 -
19 g8918.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 125 147 -
16 g8918.t6 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 148 152 -
18 g8918.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 153 172 -
13 g8918.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 173 229 -
21 g8918.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 230 250 -
15 g8918.t6 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 251 252 -
11 g8918.t6 ProSiteProfiles PS50929 ABC transporter integral membrane type-1 fused domain profile. 16 252 31.172
9 g8918.t6 SUPERFAMILY SSF90123 ABC transporter transmembrane region 13 250 4.32E-29
6 g8918.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 16 38 -
8 g8918.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 53 75 -
4 g8918.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 125 147 -
5 g8918.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 151 173 -
7 g8918.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 229 251 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0055085 transmembrane transport BP
GO:0005524 ATP binding MF
GO:0042626 ATPase-coupled transmembrane transporter activity MF
GO:0016021 integral component of membrane CC

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed