General information for g895.t14
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Diacylglycerol O-acyltransferase 2.
Parent gene
- The parent gene g895 is located on chr_3: 6754344..6755526 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g895 | g895.t14 | TTS | g895.t14 | 6753915 | 6753915 |
| chr_3 | g895 | g895.t14 | isoform | g895.t14 | 6754344 | 6755526 |
| chr_3 | g895 | g895.t14 | exon | g895.t14.exon1 | 6754344 | 6754452 |
| chr_3 | g895 | g895.t14 | cds | g895.t14.CDS1 | 6754345 | 6754452 |
| chr_3 | g895 | g895.t14 | exon | g895.t14.exon2 | 6754509 | 6754743 |
| chr_3 | g895 | g895.t14 | cds | g895.t14.CDS2 | 6754509 | 6754743 |
| chr_3 | g895 | g895.t14 | exon | g895.t14.exon3 | 6754798 | 6754929 |
| chr_3 | g895 | g895.t14 | cds | g895.t14.CDS3 | 6754798 | 6754929 |
| chr_3 | g895 | g895.t14 | exon | g895.t14.exon4 | 6755112 | 6755209 |
| chr_3 | g895 | g895.t14 | cds | g895.t14.CDS4 | 6755112 | 6755209 |
| chr_3 | g895 | g895.t14 | exon | g895.t14.exon5 | 6755383 | 6755526 |
| chr_3 | g895 | g895.t14 | cds | g895.t14.CDS5 | 6755383 | 6755526 |
| chr_3 | g895 | g895.t14 | TSS | g895.t14 | 6755612 | 6755612 |
Sequences
>g895.t14 Gene=g895 Length=718
ATGAAGAACTTAAAATCGATTTTGTCATCATCGGCATTAACGCCAACGGTGAATCGCTAT
TTGGAAGTACTCAGTGCATTGCTCTTTGGCTACATTGTGTTTTTTATTGAAGTGTCATGT
GCTTACATTGTGTATCGAATGATTGTTTATGGTGGGACAATTACTCATCTTTTTCTCGCC
GGTTATTTCATTTTCATGTTTGTTGATAGAAATGCTGATAATGACTGCAGTCGAGGTCAA
GGGTCAGACTGGATTCGTAATTTAAAATGTTTCAAATATTTTGCCAATTATTTTCCTGTG
AAACTTATCAAAACTGCTCATTTGCCGCCAAATAAAAATTATTTATTCGCCTGCTATCCA
CATGGAGTTATTGGCTGTGGCTATTTTGCAAATTTCGTGACTCATGGAACAAACTTTCAT
CAACTATTTCCTGGAATTCGGTCACGTGTTGTGTCACTTACTTATCACTTTTACATTCCA
TTTTATCGTGAATTAGTTTTAGCATGGGGTGTAATGTCACCCAAATTTATTAGCATTAAG
AATGCTTTGTCACAATCAACAGATGAAAATGCACCACTTAATTTAAAAGATGGTTATCGA
TCCACTGCCATTGTAATATCAATCGGAGGAGCTCAGGAATCATTAAATTCCAAACCTGGA
CAATACAAAATCGTTATTAAGAAACGGAAAGGCTTCATCAAAGCTGTTTTACAGACCG
>g895.t14 Gene=g895 Length=239
MKNLKSILSSSALTPTVNRYLEVLSALLFGYIVFFIEVSCAYIVYRMIVYGGTITHLFLA
GYFIFMFVDRNADNDCSRGQGSDWIRNLKCFKYFANYFPVKLIKTAHLPPNKNYLFACYP
HGVIGCGYFANFVTHGTNFHQLFPGIRSRVVSLTYHFYIPFYRELVLAWGVMSPKFISIK
NALSQSTDENAPLNLKDGYRSTAIVISIGGAQESLNSKPGQYKIVIKKRKGFIKAVLQT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g895.t14 | PANTHER | PTHR12317 | DIACYLGLYCEROL O-ACYLTRANSFERASE | 16 | 239 | 1.4E-52 |
| 3 | g895.t14 | PANTHER | PTHR12317:SF0 | DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 16 | 239 | 1.4E-52 |
| 1 | g895.t14 | Pfam | PF03982 | Diacylglycerol acyltransferase | 56 | 239 | 8.9E-44 |
| 6 | g895.t14 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 19 | - |
| 9 | g895.t14 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 20 | 44 | - |
| 8 | g895.t14 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 45 | 49 | - |
| 10 | g895.t14 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 50 | 68 | - |
| 7 | g895.t14 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 69 | 239 | - |
| 4 | g895.t14 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 23 | 45 | - |
| 5 | g895.t14 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 49 | 68 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016747 | acyltransferase activity, transferring groups other than amino-acyl groups | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed