General information for g8960.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Arginine kinase.
Parent gene
- The parent gene g8960 is located on chr_2: 34214230..34214913 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8960 | g8960.t1 | TSS | g8960.t1 | 34205498 | 34205498 |
| chr_2 | g8960 | g8960.t1 | isoform | g8960.t1 | 34205523 | 34214277 |
| chr_2 | g8960 | g8960.t1 | exon | g8960.t1.exon1 | 34205523 | 34205566 |
| chr_2 | g8960 | g8960.t1 | cds | g8960.t1.CDS1 | 34205523 | 34205566 |
| chr_2 | g8960 | g8960.t1 | exon | g8960.t1.exon2 | 34206316 | 34206402 |
| chr_2 | g8960 | g8960.t1 | cds | g8960.t1.CDS2 | 34206316 | 34206402 |
| chr_2 | g8960 | g8960.t1 | exon | g8960.t1.exon3 | 34213006 | 34213280 |
| chr_2 | g8960 | g8960.t1 | cds | g8960.t1.CDS3 | 34213006 | 34213280 |
| chr_2 | g8960 | g8960.t1 | exon | g8960.t1.exon4 | 34213336 | 34213874 |
| chr_2 | g8960 | g8960.t1 | cds | g8960.t1.CDS4 | 34213336 | 34213874 |
| chr_2 | g8960 | g8960.t1 | exon | g8960.t1.exon5 | 34213936 | 34214157 |
| chr_2 | g8960 | g8960.t1 | cds | g8960.t1.CDS5 | 34213936 | 34214157 |
| chr_2 | g8960 | g8960.t1 | exon | g8960.t1.exon6 | 34214230 | 34214277 |
| chr_2 | g8960 | g8960.t1 | cds | g8960.t1.CDS6 | 34214230 | 34214277 |
| chr_2 | g8960 | g8960.t1 | TTS | g8960.t1 | 34214625 | 34214625 |
Sequences
>g8960.t1 Gene=g8960 Length=1215
ATGTTTGGTCTTTGGTATCTTTCACTCGCAGTTAAAGAGATTTGGCACAGTTCTCGAGCG
ATTCTGTTTCAAAAACAGTTTGTGAAAAGTCACATTCAGTTGAATCTTCTTCAGTGCAAA
TTCACTCAAAGTAACAAATCCGAAATGGTTGACGCTGCAGTACTTGAGAAATTGGAATCA
GGCTTTGCCAAGCTCCAAGCCTCTGACTCGAAATCATTGCTTAAAAAGTATTTGACCAAG
GAAGTCTTTGATAATCTTAAGAACAAGAAGACCCCAACATTTGGTTCAACTTTGCTTGAT
GTCATCCAATCTGGTTTAGAAAACCACGACTCTGGCGTCGGAATTTACGCACCAGATGCC
GAGGCTTACACAACTTTCGCTGAATTGTTTGACCCAATCATTGAAGACTACCATGGTGGA
TTCAAGAAGACCGACAAGCATCCAGCAAAGGACTTTGGTGATGTAAATGCATTTGGAGAT
TTGGATCCAACTGGTGAATTCGTTGTCTCAACTCGTGTCCGTTGCGGTCGCTCAATGGAA
GGATATCCATTCAATCCATGCTTGACTGAAGCACAATACAAAGAAATGGAACAAAAGGTT
TCAACCACATTGTCAGGCTTGGAAGGTGAATTGAAGGGCAAATTCTACCCATTGACTGGC
ATGGACAAAGCTACTCAACAACAACTCATCGATGACCATTTCTTGTTCAAAGAAGGTGAT
CGCTTCTTGCAAGCTGCCAATGCTTGCCGCTATTGGCCATCAGGTCGCGGTATCTACCAC
AATGATGCCAAGACTTTCCTTGTCTGGTGCAATGAAGAAGATCACTTGCGTATCATTTCA
ATGCAAATGGGTGGCAATCTTGGTGAAGTTTTCCGTCGTTTGACAACTGCCGTTAATGAC
ATTGAGAAGCGTATTCCATTCAGCCACAACGACCGCTTGGGATTCCTCACATTCTGCCCA
ACCAACTTGGGAACAACCATCCGTGCATCAGTTCACATTAAGGTTCCAAAATTGGCTGCC
AACAAAGCTCGTCTTGAGGAAGTTGCTTCCAAATACAATTTGCAAGTTCGTGGCACACGC
GGAGAACATACAGAGGCTGAAGGTGGCATTTATGATATTTCAAACAAGAGACGTATGGGC
TTGACTGAATTCCAAGCTGTCAAGGAGATGTACGATGGAATTACTGAAATCATCAAGATT
GAAAAGTCATTGTAA
>g8960.t1 Gene=g8960 Length=404
MFGLWYLSLAVKEIWHSSRAILFQKQFVKSHIQLNLLQCKFTQSNKSEMVDAAVLEKLES
GFAKLQASDSKSLLKKYLTKEVFDNLKNKKTPTFGSTLLDVIQSGLENHDSGVGIYAPDA
EAYTTFAELFDPIIEDYHGGFKKTDKHPAKDFGDVNAFGDLDPTGEFVVSTRVRCGRSME
GYPFNPCLTEAQYKEMEQKVSTTLSGLEGELKGKFYPLTGMDKATQQQLIDDHFLFKEGD
RFLQAANACRYWPSGRGIYHNDAKTFLVWCNEEDHLRIISMQMGGNLGEVFRRLTTAVND
IEKRIPFSHNDRLGFLTFCPTNLGTTIRASVHIKVPKLAANKARLEEVASKYNLQVRGTR
GEHTEAEGGIYDISNKRRMGLTEFQAVKEMYDGITEIIKIEKSL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g8960.t1 | CDD | cd07932 | arginine_kinase_like | 57 | 404 | 0.0 |
| 9 | g8960.t1 | Gene3D | G3DSA:1.10.135.10 | Transferase Creatine Kinase | 49 | 143 | 3.4E-38 |
| 8 | g8960.t1 | Gene3D | G3DSA:3.30.590.10 | Glutamine synthetase/guanido kinase | 146 | 404 | 4.3E-120 |
| 3 | g8960.t1 | PANTHER | PTHR11547:SF38 | ARGININE KINASE-RELATED | 52 | 403 | 5.2E-154 |
| 4 | g8960.t1 | PANTHER | PTHR11547 | ARGININE OR CREATINE KINASE | 52 | 403 | 5.2E-154 |
| 1 | g8960.t1 | Pfam | PF02807 | ATP:guanido phosphotransferase, N-terminal domain | 68 | 135 | 8.6E-30 |
| 2 | g8960.t1 | Pfam | PF00217 | ATP:guanido phosphotransferase, C-terminal catalytic domain | 195 | 403 | 3.0E-86 |
| 7 | g8960.t1 | ProSitePatterns | PS00112 | Phosphagen kinase active site signature. | 319 | 325 | - |
| 11 | g8960.t1 | ProSiteProfiles | PS51509 | Phosphagen kinase N-terminal domain profile. | 54 | 139 | 26.463 |
| 10 | g8960.t1 | ProSiteProfiles | PS51510 | Phosphagen kinase C-terminal domain profile. | 167 | 404 | 88.499 |
| 5 | g8960.t1 | SUPERFAMILY | SSF48034 | Guanido kinase N-terminal domain | 66 | 148 | 3.79E-33 |
| 6 | g8960.t1 | SUPERFAMILY | SSF55931 | Glutamine synthetase/guanido kinase | 145 | 403 | 7.85E-105 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046314 | phosphocreatine biosynthetic process | BP |
| GO:0016301 | kinase activity | MF |
| GO:0004111 | creatine kinase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0016772 | transferase activity, transferring phosphorus-containing groups | MF |
KEGG
Orthology
- This transcript has been annotated with K00934 (arginine kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
