General information for g8969.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Guanylate kinase.
Parent gene
- The parent gene g8969 is located on chr_2: 34261963..34262959 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8969 | g8969.t1 | TSS | g8969.t1 | 34261822 | 34261822 |
| chr_2 | g8969 | g8969.t1 | isoform | g8969.t1 | 34261963 | 34262959 |
| chr_2 | g8969 | g8969.t1 | exon | g8969.t1.exon1 | 34261963 | 34261993 |
| chr_2 | g8969 | g8969.t1 | cds | g8969.t1.CDS1 | 34261963 | 34261993 |
| chr_2 | g8969 | g8969.t1 | exon | g8969.t1.exon2 | 34262161 | 34262235 |
| chr_2 | g8969 | g8969.t1 | cds | g8969.t1.CDS2 | 34262161 | 34262235 |
| chr_2 | g8969 | g8969.t1 | exon | g8969.t1.exon3 | 34262290 | 34262494 |
| chr_2 | g8969 | g8969.t1 | cds | g8969.t1.CDS3 | 34262290 | 34262494 |
| chr_2 | g8969 | g8969.t1 | exon | g8969.t1.exon4 | 34262560 | 34262959 |
| chr_2 | g8969 | g8969.t1 | cds | g8969.t1.CDS4 | 34262560 | 34262959 |
| chr_2 | g8969 | g8969.t1 | TTS | g8969.t1 | 34263172 | 34263172 |
Sequences
>g8969.t1 Gene=g8969 Length=711
ATGCTATCGGTCACTGTTTTAATTGCACGAGCTCTTTCAACGCTCAACAATAAAATGAAG
CCTGCCTCTATTCGGCCTCTTGTAATTTGTGGACCAAGTGGTTCTGGGAAATCAACGCTT
CTCAAGAGACTTTTTAAAGAATTTCCAAAAACATTTGGCTTTAGTGTGTCGCACACGACA
AGAAAACCAAGAAGTGACGAAGAAGATGGTGTTCATTATTATTTCGTAAATGCTGATGAC
ATGAAAGAAGCCATAAAAGCTGGTGAATTTATTGAAACGGCAGTTTTTAGCGGCAATATT
TATGGAACAAGCAAAAAAGCAATAGAGAGAGTTCAGCAAGACGGAAAAGTTTGTGTTCTT
GACATTGAAATTGAAGGAGTGAAACAAGTAAAAAATTCACATCTTAACGCCATTTTTGTG
TTCATTCAGCCACCATCAATTGAAGAACTTGAAAGACGATTGAGAGCACGAAAGACAGAA
ACAGAAGAAAGTCTTCAAAAAAGACTTAATACAGCCAAACAAGAACTTGCATTTGGTTTG
CAACCAGGCAATTTTGATATCATCATAAAAAATCATAATCTTAAACAAGCATATCACGAA
CTACGTGACTTTGTCTATGCACATCTCATCAATCAAAAAGAACAAGGACTAAATGTTTGT
GTCGAAAGTATGTCACTTGATGACAGCGATTTTATGACTAATCTTGACTAA
>g8969.t1 Gene=g8969 Length=236
MLSVTVLIARALSTLNNKMKPASIRPLVICGPSGSGKSTLLKRLFKEFPKTFGFSVSHTT
RKPRSDEEDGVHYYFVNADDMKEAIKAGEFIETAVFSGNIYGTSKKAIERVQQDGKVCVL
DIEIEGVKQVKNSHLNAIFVFIQPPSIEELERRLRARKTETEESLQKRLNTAKQELAFGL
QPGNFDIIIKNHNLKQAYHELRDFVYAHLINQKEQGLNVCVESMSLDDSDFMTNLD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g8969.t1 | CDD | cd00071 | GMPK | 26 | 202 | 6.79645E-70 |
| 6 | g8969.t1 | Coils | Coil | Coil | 147 | 175 | - |
| 4 | g8969.t1 | Gene3D | G3DSA:3.40.50.300 | - | 28 | 201 | 6.8E-72 |
| 5 | g8969.t1 | Gene3D | G3DSA:3.30.63.10 | Guanylate Kinase phosphate binding domain | 56 | 115 | 6.8E-72 |
| 2 | g8969.t1 | PANTHER | PTHR23117 | GUANYLATE KINASE-RELATED | 17 | 212 | 1.3E-90 |
| 1 | g8969.t1 | Pfam | PF00625 | Guanylate kinase | 24 | 206 | 9.1E-64 |
| 8 | g8969.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 13 | - |
| 9 | g8969.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 1 | - |
| 10 | g8969.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 2 | 9 | - |
| 11 | g8969.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 10 | 13 | - |
| 7 | g8969.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 14 | 236 | - |
| 14 | g8969.t1 | ProSitePatterns | PS00856 | Guanylate kinase-like signature. | 59 | 76 | - |
| 16 | g8969.t1 | ProSiteProfiles | PS50052 | Guanylate kinase-like domain profile. | 24 | 206 | 43.452 |
| 13 | g8969.t1 | SMART | SM00072 | gk_7 | 23 | 209 | 5.9E-80 |
| 3 | g8969.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 22 | 208 | 1.24E-64 |
| 15 | g8969.t1 | TIGRFAM | TIGR03263 | guanyl_kin: guanylate kinase | 26 | 205 | 5.2E-63 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006163 | purine nucleotide metabolic process | BP |
| GO:0004385 | guanylate kinase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00942 (guanylate kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
