General information for g8973.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin BI.
Parent gene
- The parent gene g8973 is located on chr_2: 34278698..34279809 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8973 | g8973.t3 | TSS | g8973.t3 | 34278612 | 34278612 |
| chr_2 | g8973 | g8973.t3 | isoform | g8973.t3 | 34278698 | 34279809 |
| chr_2 | g8973 | g8973.t3 | exon | g8973.t3.exon1 | 34278698 | 34279244 |
| chr_2 | g8973 | g8973.t3 | cds | g8973.t3.CDS1 | 34278698 | 34279244 |
| chr_2 | g8973 | g8973.t3 | exon | g8973.t3.exon2 | 34279371 | 34279523 |
| chr_2 | g8973 | g8973.t3 | cds | g8973.t3.CDS2 | 34279371 | 34279523 |
| chr_2 | g8973 | g8973.t3 | exon | g8973.t3.exon3 | 34279585 | 34279676 |
| chr_2 | g8973 | g8973.t3 | cds | g8973.t3.CDS3 | 34279585 | 34279607 |
| chr_2 | g8973 | g8973.t3 | exon | g8973.t3.exon4 | 34279736 | 34279809 |
| chr_2 | g8973 | g8973.t3 | TTS | g8973.t3 | 34279905 | 34279905 |
Sequences
>g8973.t3 Gene=g8973 Length=866
ATGGAAATTACACGAAACGTCATCCTGTTATTCCTCTCGAATATTTTTTTATCGCAAGTG
CCTGTAGAGAGTAATTTGATTCAAAACAAACTAGAAAATATTTTGAACAACAATGTCACT
ACTCGCTTCACACGAATCGTGGGAGGAAGCAATGCAGCTGTAGCTCAATTTCCACATGCA
GCTGCTCTTTTTCTTTTCCTTAAAACTGGAGAATCATTTTGTGGCGGCTCGATAATTCAT
GATCAATATATCCTCACGGCAGCACATTGTTTAGATGATATTTTAAGAATTGAAGTGCAA
GCTGGAACACTTAATATTTTTCAGGGTGTACCAAGATATCGTGAAACAGTTCAGCCAAGC
CATACTCGTCAACATCATCAATATGATAGTGACACTTTGAGAAATGACATTGGTTTAATT
TATTTAATGCGACCAATTCCTTTTTCACCTGCTATTAGACCCATCGCACTTCCGTCAAGA
GTTACTGCTACAAGTACTTTCCAGGGTATGCTGACAACAGTAATTGGTTGGGGTCGATTT
GAAGATGCAAAACAATCTTTGTCGGACACTCTGCAATTTGTGAGACTTCCAATAGTGCAA
GATCGACAATGCACAACAGTTTACAATCAATTCTATAATTTTTTTGATACTCGAACAAAT
ATTTGTGCAAGCGGTAGTCACGCAAGATCGACTTGTAATGAGGTGATAGTGGAGGTTCTA
TGACAATCGACACAAACGGCCGTCCTACTCTGATTGGTATTGTTTCTTTTGGAAGTTCCA
CATGTGAAGGAGGTCTTCCAGTTGCGATGACACGCGTTACTGCCTATCTTGATTGGATTG
CTGCAAATTCACTTGTACGCTGCTAA
>g8973.t3 Gene=g8973 Length=240
MEITRNVILLFLSNIFLSQVPVESNLIQNKLENILNNNVTTRFTRIVGGSNAAVAQFPHA
AALFLFLKTGESFCGGSIIHDQYILTAAHCLDDILRIEVQAGTLNIFQGVPRYRETVQPS
HTRQHHQYDSDTLRNDIGLIYLMRPIPFSPAIRPIALPSRVTATSTFQGMLTTVIGWGRF
EDAKQSLSDTLQFVRLPIVQDRQCTTVYNQFYNFFDTRTNICASGSHARSTCNEVIVEVL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g8973.t3 | CDD | cd00190 | Tryp_SPc | 46 | 233 | 4.83688E-50 |
| 8 | g8973.t3 | Gene3D | G3DSA:2.40.10.10 | - | 39 | 235 | 6.4E-43 |
| 2 | g8973.t3 | PANTHER | PTHR24260 | - | 39 | 233 | 1.8E-41 |
| 3 | g8973.t3 | PANTHER | PTHR24260:SF90 | AT07769P-RELATED | 39 | 233 | 1.8E-41 |
| 5 | g8973.t3 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 75 | 90 | 5.4E-7 |
| 4 | g8973.t3 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 132 | 146 | 5.4E-7 |
| 1 | g8973.t3 | Pfam | PF00089 | Trypsin | 46 | 233 | 4.3E-32 |
| 10 | g8973.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 24 | - |
| 11 | g8973.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 12 | g8973.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 17 | - |
| 13 | g8973.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 24 | - |
| 9 | g8973.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 25 | 240 | - |
| 16 | g8973.t3 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 85 | 90 | - |
| 17 | g8973.t3 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 46 | 233 | 19.61 |
| 15 | g8973.t3 | SMART | SM00020 | trypsin_2 | 45 | 236 | 9.1E-29 |
| 6 | g8973.t3 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 31 | 233 | 2.36E-50 |
| 7 | g8973.t3 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 24 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed