General information for g9015.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Neuropeptide CCHamide-2 receptor.
Parent gene
- The parent gene g9015 is located on chr_2: 34602290..34608339 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g9015 | g9015.t1 | isoform | g9015.t1 | 34602290 | 34608339 |
| chr_2 | g9015 | g9015.t1 | exon | g9015.t1.exon1 | 34602290 | 34602488 |
| chr_2 | g9015 | g9015.t1 | cds | g9015.t1.CDS1 | 34602290 | 34602488 |
| chr_2 | g9015 | g9015.t1 | exon | g9015.t1.exon2 | 34602554 | 34602674 |
| chr_2 | g9015 | g9015.t1 | cds | g9015.t1.CDS2 | 34602554 | 34602674 |
| chr_2 | g9015 | g9015.t1 | exon | g9015.t1.exon3 | 34603088 | 34603281 |
| chr_2 | g9015 | g9015.t1 | cds | g9015.t1.CDS3 | 34603088 | 34603281 |
| chr_2 | g9015 | g9015.t1 | exon | g9015.t1.exon4 | 34603342 | 34603725 |
| chr_2 | g9015 | g9015.t1 | cds | g9015.t1.CDS4 | 34603342 | 34603725 |
| chr_2 | g9015 | g9015.t1 | exon | g9015.t1.exon5 | 34608152 | 34608339 |
| chr_2 | g9015 | g9015.t1 | cds | g9015.t1.CDS5 | 34608152 | 34608339 |
| chr_2 | g9015 | g9015.t1 | TSS | g9015.t1 | NA | NA |
| chr_2 | g9015 | g9015.t1 | TTS | g9015.t1 | NA | NA |
Sequences
>g9015.t1 Gene=g9015 Length=1086
ATGGATAATTATACGGACATCAGTAATCACAGCCTCGAAGATGAGGATTTAATACCATAC
TCTGCTCGAATAGAAAATAGAATTGTTTATATTCTATTTTCATTAATTTTTATTGTTGGA
ATACTAGGCAACTCAACAGTAATTTTAATTTTTTTAAGACACATTTCAATGAGAAACGTT
CCTAACACTTATATATTTTCATTAGCTTTTGGCGATCTTTTAGTCATATTAATTACAGTA
CCTTGTGCAGGACTCATATTTGTCTATGATCAGTGGCCATGGGATGGTGAAATTGGTGAA
ATCATTTGTCGTGTTAGTGAATTTGCTAAAGACATATCAATTGGAGTTTCTATATTTACG
CTTGTTGCCTTAGCATTCGATAGATATACTGCCATTGTATGCCCTCTAAAAAAATTGAGA
GCGAGGTCAAAAATGGTGATAGTGATAATAATTTTCACTTGGATATTAGCAATTCTCGTT
GCTTTGCCTGCAGTTATCGTTTCTAGAGTTGTTATAACTGCTAATGTCAAATATTGTACA
CCTTTTGGTCATTATGGAAAAACTTACAGCAAGTATGTAACAATTATCAAAGCATCAATC
TACTATTTCCTTCCACTTTCCATCATTGCAATTCTATACACATTGATGGCAAAAAAACTT
CGAAATAGTGCACGTGAAGTCCAAACAATAACTGGCAATCAACTGAAAAGCTCACAGGCC
CAAAATAGAAGGCACGTGGCCAGGATGGTGATTGTATTTATTTTAGTCTTCGTCATTTGT
TTCTATCCTCATTGGATGTTCCAGTTATGGTTTCAACTCAGCGAAAATGCGGAAGAAAAT
TATGATATGACTTGGCACATTATAAGAATCGTTGGATTTTGTTTATCATTTATCAACTCA
ACATTAAATCCACTCGCTTTATATTTTGTGTCTAGCGTATTTCGAACACACTTTCATAAC
TATTTATGTTGCAAGTCATATTCTGTTGAACATCACAATGTTTCATTTTGCAGAAATTTA
TCGAAATACAATATTAAAAATCCTGAAACAACAATTATCGATGCTACTGATTTAACAATC
AACTGA
>g9015.t1 Gene=g9015 Length=361
MDNYTDISNHSLEDEDLIPYSARIENRIVYILFSLIFIVGILGNSTVILIFLRHISMRNV
PNTYIFSLAFGDLLVILITVPCAGLIFVYDQWPWDGEIGEIICRVSEFAKDISIGVSIFT
LVALAFDRYTAIVCPLKKLRARSKMVIVIIIFTWILAILVALPAVIVSRVVITANVKYCT
PFGHYGKTYSKYVTIIKASIYYFLPLSIIAILYTLMAKKLRNSAREVQTITGNQLKSSQA
QNRRHVARMVIVFILVFVICFYPHWMFQLWFQLSENAEENYDMTWHIIRIVGFCLSFINS
TLNPLALYFVSSVFRTHFHNYLCCKSYSVEHHNVSFCRNLSKYNIKNPETTIIDATDLTI
N
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g9015.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 2 | 357 | 5.7E-74 |
| 2 | g9015.t1 | PANTHER | PTHR45695 | LEUCOKININ RECEPTOR-RELATED | 5 | 332 | 4.5E-128 |
| 3 | g9015.t1 | PANTHER | PTHR45695:SF10 | NEUROPEPTIDE CCHAMIDE-2 RECEPTOR | 5 | 332 | 4.5E-128 |
| 6 | g9015.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 28 | 52 | 1.6E-39 |
| 7 | g9015.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 61 | 82 | 1.6E-39 |
| 10 | g9015.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 110 | 132 | 1.6E-39 |
| 9 | g9015.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 145 | 166 | 1.6E-39 |
| 5 | g9015.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 194 | 217 | 1.6E-39 |
| 8 | g9015.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 246 | 270 | 1.6E-39 |
| 4 | g9015.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 289 | 315 | 1.6E-39 |
| 1 | g9015.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 43 | 305 | 4.0E-45 |
| 18 | g9015.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 27 | - |
| 21 | g9015.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 28 | 52 | - |
| 15 | g9015.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 53 | 63 | - |
| 27 | g9015.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 64 | 88 | - |
| 20 | g9015.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 89 | 107 | - |
| 23 | g9015.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 108 | 126 | - |
| 14 | g9015.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 127 | 145 | - |
| 24 | g9015.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 146 | 172 | - |
| 19 | g9015.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 173 | 191 | - |
| 26 | g9015.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 192 | 215 | - |
| 13 | g9015.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 216 | 245 | - |
| 25 | g9015.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 246 | 267 | - |
| 17 | g9015.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 268 | 286 | - |
| 22 | g9015.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 287 | 310 | - |
| 16 | g9015.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 311 | 361 | - |
| 35 | g9015.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 116 | 132 | - |
| 36 | g9015.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 43 | 307 | 41.977 |
| 11 | g9015.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 2 | 339 | 1.83E-63 |
| 34 | g9015.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 52 | - |
| 31 | g9015.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 65 | 87 | - |
| 29 | g9015.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 112 | 134 | - |
| 33 | g9015.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 146 | 168 | - |
| 28 | g9015.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 193 | 215 | - |
| 32 | g9015.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 250 | 272 | - |
| 30 | g9015.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 287 | 309 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed