General information for g9032.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Transient receptor potential cation channel subfamily A member 1.
Parent gene
- The parent gene g9032 is located on chr_2: 34749129..34758731 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g9032 | g9032.t2 | isoform | g9032.t2 | 34749129 | 34752019 |
| chr_2 | g9032 | g9032.t2 | exon | g9032.t2.exon1 | 34749129 | 34752019 |
| chr_2 | g9032 | g9032.t2 | cds | g9032.t2.CDS1 | 34750357 | 34751037 |
| chr_2 | g9032 | g9032.t2 | TSS | g9032.t2 | NA | NA |
| chr_2 | g9032 | g9032.t2 | TTS | g9032.t2 | NA | NA |
Sequences
>g9032.t2 Gene=g9032 Length=2891
ATAAGATATTCATTTAGTTGTCTTCATATTCCACAATATTATGCGCAAATTGATGAGAAA
ACTGGTGATGCCGTTAATATCTCTGAGCCAATTCCTTTGCCAGCACTCAACGTAAGCTCA
TAGCATTGAGTTTATCCAATATTTTTCTGTTTTAATTAATGGCATGCCTTCGTAGATATT
TAACAAATTTTTTAGCTTTTGTTTTATAATCTTGGCAGTTTTATTTTACATTCAACTCTG
TTTGTTTTGGTTTGTAGATTAAATATTCTTTTGAAGTTTTCGAAAAATCTAAGGCCAAAA
TTGCATTCACTCGTGAACAGTTGAATGATCCGAATTGGATGCCTCCTCCTTTGGTAGTTG
TCAATGTGAGTTTTATTTTTTTGTGTACTTGTGGTTTTCTTTTTTTGCTTTATTTGTTTC
TTTTTATTCAAATAATCTTTTTTTTTTTAAGCTTGACACATTTGACTTCTACTGATAGAA
AGTATTGTACAATAATAAATATTTACAAAAGAAAACAAATATTCTTGGCTTAGCATGAGC
AAATAATTTAAATTTCTAACTATGGTCATTGCATGATGTAAAATTTTATTTAGAAAACTT
GAAACTTTTTAGTTCATGCATGCATCCTTAATTATTTTATTCATTCATTGCTGTGTTAAT
CTTTATGTTGTATTTCATATTCATGATGAAGAGCATCAAAATGGTAATTGAAATTTATTT
ATATTATGCATTCAGGCAATGGTTAGTCATGGTCGTGTTGAACTTCTTGCTCATCCACTG
AGTCAAAAATACCTTCAAATGAAATGGAACAGCTATGGAAAGTATTTTCACATTGCAAAT
TTGCTTCTTTATAGCATTTTGTTATTTTTTATCACGATGTATTCGTCTCTTTTAATGAAA
AATGGTATGAAAAGGGTGAGTATTACTCTTGCACATATATGCATGTTTTTTCCTGATTTT
TCTCTATTTGCAGTCATCACTTATGCAACAGCAGTATATCGAACTAACAAATGGAACCAA
CAACAAAACTAAAGCATTGGAAAATGAAAATTTTAAAATTGACATTGATTCTGGCATGTT
CGTGTCTGGGACTGTAATTTTTGTTTACATTCTAGCAAGTACTATTCGAGAAATCATTCA
AGCAACTCAAAAACGTCATCATTATTTGCTCGATCCAATGAATTTAGTCTCATGGATTCT
ATATGGAAATGCACTTATAATGATATCACCAATTTTCACAAATGGTCATGTTTGTGATTA
TTTATTCTCAGCTGCATCACTTACAACTTTCTTATCTTGGTTCAATCTTTTGCTACATCT
TCAACGATTTGATCAAATTGGGATTTATGTCGTGATGTTTCTCGAAATATTGCAAACATT
GATAAAAGTTCTTATTGTATTTTCGATTTTGATTGTCGCATTTGGACTTGCATTTTTCAT
TTTACTCTCGAACATCCAACATCCACAGCCTAATCATCTCTCATTTACTACGATTCCAAT
GTCTCTTATGAGGACATTTTCAATGATGTTGAGTGAAGTTGATATTGTTGGCACTTTTAT
TCAACCATATCATTTGAACCACTTACCTTATCCATATGCATCATTTATTATTCTCTGTGA
GTTAAAACATCCTTTTGTGATTAATTTTAATTTTGGCCATTAATGAAATTATTTTTGCAG
TTTTATTCATGATACTTATGCCAATTCTATTGATGAATTTACTTATTGGTTTGGCTGTTG
GTGATATCGAAAGTGTAAGACGAAATGCACAATTAAAACGTTTAGCCATGCAAGTATGTG
GTTTGCTTTTCTTTATTATGATTGTGTCTCATTAGCTTTAATTATTTTTTTATTAATTGA
TGTAGGTGGTCCTACATACAGAACTTGAACGTAAATTACCACAAATGTGGCTAGAGAAAA
TCGACAAGCACGAATTAATTGAGTATCCGAATGAAGTATGATTGATAAGTGTCACTTTTA
CTTTACAATTAATAAAATATTTTTATATTAGGCAAAATGCAAATTGACACTTCTTGATTC
TATTTTGAGAAAATGGTTTTGTAATCCATTCAATGAAAATGAAAATGGTAAGTAAAATTT
GTAAAATTAAAATTAATAAAAATAATTAATAAATTTTACAGCTGGTCTTGAACTTGTACT
TGACAATGCAAATGAGGATTTTATGTTATATGAAATTGAAAAACAAAAGATGAAACTAAA
AGAAATGTCGAAAATGATTGAACAACAACATCAATTACTTCGCTTGATTGTGCAGGTAAT
ATTTCTTAATTACTTTATAATAATGTTTACATTAGGTAGGATTCAGACTTGCATCCTTTT
TTTACTTGATATGCGCATATTTTTTGTCAAGTTGATTTCAAATTGACTCAAACAAAAATT
AAATAATTTATTCAGATTGAATTTAATTCAGTAAAAATACATAGTAAATTAAAAAATCAT
TGACTTTCATACAAATTTACTGGATTTCAAATAAATTAAATAAAATCTGAATGAGTTAAA
GACATTTTTGTGTAAGTCAACTTGACAAAAAATATGCATGCAAATCTGAACCAGAAATTT
TTTCAAAAAAAGTTCGTTATGTTTGAAGGTCCCTAATTTAATTTTGAATAATTCGAACAC
AGGGATCAGTCCTTGTTTTATTCGAAGGAAAATGGTAGTTTACATTAAAAATAATTTTAA
AATAATTTAAATTTTCTCTACAGAAAATGGAAATCAAATCAGAAGCAGACGACGTCGATG
AAGGTGTGCCTCTAAGGGAAAGCCGTCCTCTTTCAGCTATTTCAAATGAACATGTTACAC
GTTGGACATCACCAAGAATAAGAAAAAAGCTAAGAGCTGTGAAAAGTTTCAGCAAAAGTT
TTAATCAATAA
>g9032.t2 Gene=g9032 Length=226
MQQQYIELTNGTNNKTKALENENFKIDIDSGMFVSGTVIFVYILASTIREIIQATQKRHH
YLLDPMNLVSWILYGNALIMISPIFTNGHVCDYLFSAASLTTFLSWFNLLLHLQRFDQIG
IYVVMFLEILQTLIKVLIVFSILIVAFGLAFFILLSNIQHPQPNHLSFTTIPMSLMRTFS
MMLSEVDIVGTFIQPYHLNHLPYPYASFIILCELKHPFVINFNFGH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g9032.t2 | PANTHER | PTHR47143 | TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL PROTEIN PAINLESS | 3 | 211 | 5.9E-35 |
| 1 | g9032.t2 | Pfam | PF00520 | Ion transport protein | 40 | 190 | 4.2E-11 |
| 10 | g9032.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 29 | - |
| 12 | g9032.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 48 | - |
| 8 | g9032.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 49 | 67 | - |
| 15 | g9032.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 68 | 87 | - |
| 9 | g9032.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 88 | 92 | - |
| 13 | g9032.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 93 | 111 | - |
| 7 | g9032.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 112 | 131 | - |
| 14 | g9032.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 132 | 155 | - |
| 11 | g9032.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 156 | 226 | - |
| 4 | g9032.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 31 | 53 | - |
| 6 | g9032.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 68 | 87 | - |
| 5 | g9032.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 94 | 116 | - |
| 3 | g9032.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 136 | 158 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0055085 | transmembrane transport | BP |
| GO:0005216 | ion channel activity | MF |
| GO:0006811 | ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
