General information for g9099.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 5’-AMP-activated protein kinase subunit gamma-2.
Parent gene
- The parent gene g9099 is located on chr_1: 110384..115146 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9099 | g9099.t3 | TTS | g9099.t3 | 109889 | 109889 |
| chr_1 | g9099 | g9099.t3 | isoform | g9099.t3 | 110384 | 114364 |
| chr_1 | g9099 | g9099.t3 | exon | g9099.t3.exon1 | 110384 | 111049 |
| chr_1 | g9099 | g9099.t3 | cds | g9099.t3.CDS1 | 110384 | 111049 |
| chr_1 | g9099 | g9099.t3 | exon | g9099.t3.exon2 | 111116 | 111187 |
| chr_1 | g9099 | g9099.t3 | cds | g9099.t3.CDS2 | 111116 | 111187 |
| chr_1 | g9099 | g9099.t3 | exon | g9099.t3.exon3 | 111245 | 111376 |
| chr_1 | g9099 | g9099.t3 | cds | g9099.t3.CDS3 | 111245 | 111376 |
| chr_1 | g9099 | g9099.t3 | exon | g9099.t3.exon4 | 111438 | 111497 |
| chr_1 | g9099 | g9099.t3 | cds | g9099.t3.CDS4 | 111438 | 111497 |
| chr_1 | g9099 | g9099.t3 | exon | g9099.t3.exon5 | 111555 | 111670 |
| chr_1 | g9099 | g9099.t3 | cds | g9099.t3.CDS5 | 111555 | 111670 |
| chr_1 | g9099 | g9099.t3 | exon | g9099.t3.exon6 | 111731 | 112021 |
| chr_1 | g9099 | g9099.t3 | cds | g9099.t3.CDS6 | 111731 | 111833 |
| chr_1 | g9099 | g9099.t3 | exon | g9099.t3.exon7 | 114316 | 114364 |
| chr_1 | g9099 | g9099.t3 | TSS | g9099.t3 | NA | NA |
Sequences
>g9099.t3 Gene=g9099 Length=1386
TTACCTGTTGCAGATCCGTTCCTAGAAAAAGTTAATTTGTCTGATTTAGAAGAAGATGAA
TCACAAATTTTTGTTAAATTCTTCCGCTTTCATAAATGTTATGATTTAATACCGACATCA
GCAAAGTTAGTTGTATTTGATACTCAACTATTAGTCAAGAAAGCGTTTTATGCTTTGGTA
TATAATGGAGTACGAGCAGCACCTCTCTGGGACTCTGAGCAACAAAAATTTGTTGGAATG
CTTACAATAACAGATTTTATAAAAATCCTCAAAATGTACTACACATCACCCAACTGCTCA
ATGGATCAACTTGAAGAACATAAACTTGCAACTTGGAGAAATGTTCTTAAAGACCATGTG
AATTTAATAAGTATTAGTCCTGATGCATCTCTTTATGATGCTATTAAAACTTTAATACAT
AATCGCATTCATCGCTTACCAGTTATTGATCCATTGACCGGAAATGTTCTTTATATTTTG
ACCCATAAACGAATACTTCGTTTTCTATTTCTTTACATTAATGAACTTCCAAAACCATCA
TATATGCATAAAACATTGCGAGAATTGAAAATAGGCACATACGACAATATTGAAACATCA
ACAGAAGATACAAGCATCATTACTGCTCTAAAAAAATTTGTCGATAGGCGAGTGTCAGCA
TTACCAATGGTCGATTCAGAGGGCCGCCTTACAGACATTTACGCAAAGTTTGATGTGATT
AATCTAGCAGCCGAGAAAACTTACAACGATTTAGATGTAAGTCTAAAAAAGGCTAATGAG
CATCGCAACGCGTGGTTCGAAGGCGTGCAAAAGTGCAATTTGGATGAAACACTGTACACT
GTGATGGAAAGAATTGTTCGTGCTGAAGTACATCGATTGGTAGTAGTTGATGAAGATCAA
AAAGTAATTGGCATCATTTCATTATCCGATATTCTTCTTTATCTCGTATTACGATGTGCG
GGTGATGGTCTTGGTGATTCTGAATCATTAAGAGCAACAGATCCGATTGTTCAGACACGT
GAACGTACCGACAGTACGAGTCAATATATGTTTAACAATAGAAGTAATGAGAGTATTGAG
GAGGAAACTAACAATGATTTAGAGGCGGAGAGTGAAGATCAAAGTGGTGGTGATAGTAAT
AATAATGTAAAAAATGATGAGGAAGCAGAAATGGCAGAAGAAGCTTTGCCATCAACCGAT
GACAAGACTGATGACGACAATGAGAACAACAATGTTAAAACTCCACCTGAAATTTCAAAA
GATGAAGTGATACACATTGAGGAAGAAGAAGATCAAGTCAACTTGTCAACGATGAACAAC
AATGGCAGCAATAACTTAAATAACACACTAAATATTCAACGCGAAATGGCGATTGTCAGC
GAATGA
>g9099.t3 Gene=g9099 Length=382
MLTITDFIKILKMYYTSPNCSMDQLEEHKLATWRNVLKDHVNLISISPDASLYDAIKTLI
HNRIHRLPVIDPLTGNVLYILTHKRILRFLFLYINELPKPSYMHKTLRELKIGTYDNIET
STEDTSIITALKKFVDRRVSALPMVDSEGRLTDIYAKFDVINLAAEKTYNDLDVSLKKAN
EHRNAWFEGVQKCNLDETLYTVMERIVRAEVHRLVVVDEDQKVIGIISLSDILLYLVLRC
AGDGLGDSESLRATDPIVQTRERTDSTSQYMFNNRSNESIEEETNNDLEAESEDQSGGDS
NNNVKNDEEAEMAEEALPSTDDKTDDDNENNNVKTPPEISKDEVIHIEEEEDQVNLSTMN
NNGSNNLNNTLNIQREMAIVSE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g9099.t3 | CDD | cd04641 | CBS_euAMPK_gamma-like_repeat2 | 114 | 237 | 4.09669E-68 |
| 8 | g9099.t3 | Gene3D | G3DSA:3.10.580.10 | - | 1 | 94 | 3.5E-32 |
| 9 | g9099.t3 | Gene3D | G3DSA:3.10.580.10 | - | 95 | 239 | 3.4E-58 |
| 14 | g9099.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 256 | 279 | - |
| 15 | g9099.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 256 | 343 | - |
| 16 | g9099.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 304 | 332 | - |
| 4 | g9099.t3 | PANTHER | PTHR13780 | AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT | 1 | 242 | 1.8E-108 |
| 5 | g9099.t3 | PANTHER | PTHR13780:SF122 | 5’-AMP-ACTIVATED PROTEIN KINASE SUBUNIT GAMMA-2 | 1 | 242 | 1.8E-108 |
| 3 | g9099.t3 | Pfam | PF00571 | CBS domain | 42 | 90 | 8.4E-8 |
| 2 | g9099.t3 | Pfam | PF00571 | CBS domain | 122 | 162 | 6.9E-4 |
| 1 | g9099.t3 | Pfam | PF00571 | CBS domain | 192 | 237 | 2.1E-11 |
| 19 | g9099.t3 | ProSiteProfiles | PS51371 | CBS domain profile. | 36 | 97 | 12.1 |
| 18 | g9099.t3 | ProSiteProfiles | PS51371 | CBS domain profile. | 112 | 172 | 9.595 |
| 17 | g9099.t3 | ProSiteProfiles | PS51371 | CBS domain profile. | 186 | 248 | 10.954 |
| 12 | g9099.t3 | SMART | SM00116 | cbs_1 | 42 | 91 | 2.0E-8 |
| 13 | g9099.t3 | SMART | SM00116 | cbs_1 | 117 | 165 | 1.1E-4 |
| 11 | g9099.t3 | SMART | SM00116 | cbs_1 | 189 | 237 | 5.9E-8 |
| 7 | g9099.t3 | SUPERFAMILY | SSF54631 | CBS-domain pair | 1 | 99 | 1.34E-19 |
| 6 | g9099.t3 | SUPERFAMILY | SSF54631 | CBS-domain pair | 102 | 240 | 3.2E-29 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0071900 | regulation of protein serine/threonine kinase activity | BP |
| GO:0032559 | adenyl ribonucleotide binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
