General information for g921.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin-1.
Parent gene
- The parent gene g921 is located on chr_3: 6866430..6867884 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g921 | g921.t10 | TSS | g921.t10 | 6865818 | 6865818 |
| chr_3 | g921 | g921.t10 | isoform | g921.t10 | 6865841 | 6866908 |
| chr_3 | g921 | g921.t10 | exon | g921.t10.exon1 | 6865841 | 6865895 |
| chr_3 | g921 | g921.t10 | cds | g921.t10.CDS1 | 6865841 | 6865895 |
| chr_3 | g921 | g921.t10 | exon | g921.t10.exon2 | 6865954 | 6866001 |
| chr_3 | g921 | g921.t10 | cds | g921.t10.CDS2 | 6865954 | 6866001 |
| chr_3 | g921 | g921.t10 | exon | g921.t10.exon3 | 6866074 | 6866160 |
| chr_3 | g921 | g921.t10 | cds | g921.t10.CDS3 | 6866074 | 6866160 |
| chr_3 | g921 | g921.t10 | exon | g921.t10.exon4 | 6866224 | 6866365 |
| chr_3 | g921 | g921.t10 | cds | g921.t10.CDS4 | 6866224 | 6866365 |
| chr_3 | g921 | g921.t10 | exon | g921.t10.exon5 | 6866419 | 6866782 |
| chr_3 | g921 | g921.t10 | cds | g921.t10.CDS5 | 6866419 | 6866782 |
| chr_3 | g921 | g921.t10 | exon | g921.t10.exon6 | 6866846 | 6866908 |
| chr_3 | g921 | g921.t10 | cds | g921.t10.CDS6 | 6866846 | 6866908 |
| chr_3 | g921 | g921.t10 | TTS | g921.t10 | 6866992 | 6866992 |
Sequences
>g921.t10 Gene=g921 Length=759
ATGATTCGTCAAGTGCTACTTTTTGCTTTGTTTGTTTCTGTGGCTTTTGCTGCACCACAA
AATAGAGAAAGAAGAATTGTTGGTGGAGTTGATAATGTCTTGGTACCATGGGCTGTAAGC
TTAAGAACAATAGCAGGACTTCATTTTTGTACAGGATCAGTTCTCAATAATTGGTATGTT
ATCACATCAGCTCATTGCATCGATGGACGAGGAGCATCAACAATTCATTTAGTTTTGGGT
AGAATCACACTTGATGGATTAACTGGAATGAATCGTCAAGGTTTTAGAGTTATTATTCAT
CCTGCATATGATGTTTCAACTCAAAATGCTGATGTTGGTTTAATTCAAGTATCAGTAATA
ATAACATTCAATGCAAATGTGCAAGCAATTTCATTAGGTGATACAATGATTGGAACTGGA
GTTTCTGCAAGAGTGAGTGGTTGGGGTTCAACAGAACAAGACTTTGGACCAACTTCGAAT
AATCTCAGATCACTTGATACAACAACACTTTCATTAAATGAATGTCAAGATAGACATACA
TGGTGGAATGCTGAAAGAATAAATATGAACCATCTCTGTACAAATAATGGAGTTAATGAA
GGTTTCTGTATGACAGGAGCTGGTGGATCTCTTGTCAGTAATTCACAATTAATCGGTGTC
GCTTCATGGAATGTACCTTGTGCTTTAGGCTATCCCGATGTTTATGTGAGAATATCAACA
TTCAGAACTTGGATAATGTCACAAATCGGTTCTCAATAA
>g921.t10 Gene=g921 Length=252
MIRQVLLFALFVSVAFAAPQNRERRIVGGVDNVLVPWAVSLRTIAGLHFCTGSVLNNWYV
ITSAHCIDGRGASTIHLVLGRITLDGLTGMNRQGFRVIIHPAYDVSTQNADVGLIQVSVI
ITFNANVQAISLGDTMIGTGVSARVSGWGSTEQDFGPTSNNLRSLDTTTLSLNECQDRHT
WWNAERINMNHLCTNNGVNEGFCMTGAGGSLVSNSQLIGVASWNVPCALGYPDVYVRIST
FRTWIMSQIGSQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g921.t10 | CDD | cd00190 | Tryp_SPc | 26 | 248 | 4.19427E-49 |
| 7 | g921.t10 | Gene3D | G3DSA:2.40.10.10 | - | 35 | 239 | 2.8E-45 |
| 8 | g921.t10 | Gene3D | G3DSA:2.40.10.10 | - | 36 | 245 | 2.8E-45 |
| 2 | g921.t10 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 6 | 249 | 3.2E-47 |
| 3 | g921.t10 | PANTHER | PTHR24276:SF78 | AT20289P-RELATED | 6 | 249 | 3.2E-47 |
| 1 | g921.t10 | Pfam | PF00089 | Trypsin | 35 | 245 | 5.0E-38 |
| 10 | g921.t10 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 11 | g921.t10 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 12 | g921.t10 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 12 | - |
| 13 | g921.t10 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 9 | g921.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 252 | - |
| 17 | g921.t10 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 26 | 250 | 24.168 |
| 16 | g921.t10 | SMART | SM00020 | trypsin_2 | 25 | 245 | 7.4E-35 |
| 4 | g921.t10 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 6 | 250 | 3.21E-52 |
| 6 | g921.t10 | SignalP_EUK | SignalP-TM | SignalP-TM | 1 | 17 | - |
| 15 | g921.t10 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
| 5 | g921.t10 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed