General information for g9236.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable asparagine synthetase [glutamine-hydrolyzing].
Parent gene
- The parent gene g9236 is located on chr_1: 1019546..1022074 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9236 | g9236.t1 | isoform | g9236.t1 | 1019546 | 1022074 |
| chr_1 | g9236 | g9236.t1 | exon | g9236.t1.exon1 | 1019546 | 1020170 |
| chr_1 | g9236 | g9236.t1 | cds | g9236.t1.CDS1 | 1019546 | 1020170 |
| chr_1 | g9236 | g9236.t1 | exon | g9236.t1.exon2 | 1020972 | 1022074 |
| chr_1 | g9236 | g9236.t1 | cds | g9236.t1.CDS2 | 1020972 | 1022074 |
| chr_1 | g9236 | g9236.t1 | TSS | g9236.t1 | NA | NA |
| chr_1 | g9236 | g9236.t1 | TTS | g9236.t1 | NA | NA |
Sequences
>g9236.t1 Gene=g9236 Length=1728
ATGTGTGGAATATTTGCAATTTTCGGAAATGCATGCAACAATCCTGAATGTCAGCAACAA
CAAAGTGCAAATGGTTTTAATGGTGAAAGTAAAATGATAAAAACAACAAATGAATTTCCT
GGTGAAACATTGAGAGAAATGGCATACAGATGCTCAACTAAACAACGTCATCGTGGACCT
GATAAGACTGGAATTATTGAATTCAAAGAAGAAAATTCTGTGATGGTTCATGAAAGATTA
AGAATTATTGGTGTTGCACATGGTGATCAACCATTGCAAAATGATAATGGAAATATTACT
TTAATAGCAAATGGTGAAATTTACAACTTCATTAAGCTTTCCAATGAGCTGTCAGCATTT
TACAAGAAACCATATGAGCCAAGAAGTGATTGTGATGTAATCATTGGTTTGTATGAAAAG
TATGGAGATGATTTGGTACACCATTTGACGGGAATGTATGCATTTGTATTATATGATCGT
AAAAATAATAAAACTCTTGCTGCACGTTGTCCTTTTGGAATAATCTCAATGTATATTGGT
GAGGATGTTCATGGAAATATTTGGATAGCAAGTGAGATGAAGTGTTTGGTTGAAAAGTGT
CCATATGTTCAAAACTTTCCCATTGGAAGTCTCTTTTCGCATCAACTCGGCACATCCAAC
AAGTGGAAGTTTATAAAGTACTTCAAGGAGGATTGGATTACGAAGGTGCCGCAGTTGAAG
GTTGATTTAGTGCACTTTAGGGAGACACTTGAATCTGCAGTACGATCACACTTGCAATGT
GAAGTTTCGTTCGGTGCATTACTCTCTGGTGGTGTCGATTCTTCATTGATTTCTTCCATT
GCGACAAAAATCATGCGAGAAAAAAATCCCAACTTTAAGCTTCAGACATTCAGTGTTGGT
TTAACTGGTGCTCCTGATTTCAAATATAGTCAAATAGTTGCAGATTATATTGGCAGTGAA
CATCATGAAATTCATTTTACTGTCGAAGAAGGTCTCGATTGTATTCGCGATATAATTTAT
CATCTTGAAACATACGACATCACAACAATTCGTGCTTCAATTCCAATGTATATTATGATG
CGTTATATTAAAAGTCGTGGATTAAAGATGGTTCTATCAGGTGAAGGTGCAGATGAGATT
CTTGGTGGCTATTTATATTTCCATAGTGCACCTAATGCTGAAGAATTTCATTATGAGACA
GTTTCGCGTGTTTTACATTTATGGTCAAATGATTGCCTACGTGCTAATAAATCTTCACTT
GCGTGGGGTGTTGAGCTACGTGTGCCATTTCTTGATACTAATTTTGTCAACTATTCTATG
AGTGTGCGACCAGAAGATCGAATGATTAGCACTGATGAGAAAAAAATTGAAAAATTCATT
TTACGAAAAGCATTTTCAATGAATTACTTGCCTGATGAAGTACTTTGGAGACAAAAAGAG
CAATTTTCTGATGGTGTAGGCTATAATTGGATCGATACGATCCGCACCTACGCTAGTAGT
CATATCAACGATGACGAATTTGAAAAGGCCAAAGAACTCTATCCATTCAATACACCATTG
AGCAAAGAAGGATTTTACTATAGAAAAATATTTGAACGCATTTTTCCAAATCAATCATGT
GTGAAAACAGTTAAGCAGTGGATTCCACGTCAAGATTGGGGATGTGCTTTGGATCCATCC
GGACGCGCACAATTGGTTCATGTTGCCAGCAAAAAGATAAATGAATAA
>g9236.t1 Gene=g9236 Length=575
MCGIFAIFGNACNNPECQQQQSANGFNGESKMIKTTNEFPGETLREMAYRCSTKQRHRGP
DKTGIIEFKEENSVMVHERLRIIGVAHGDQPLQNDNGNITLIANGEIYNFIKLSNELSAF
YKKPYEPRSDCDVIIGLYEKYGDDLVHHLTGMYAFVLYDRKNNKTLAARCPFGIISMYIG
EDVHGNIWIASEMKCLVEKCPYVQNFPIGSLFSHQLGTSNKWKFIKYFKEDWITKVPQLK
VDLVHFRETLESAVRSHLQCEVSFGALLSGGVDSSLISSIATKIMREKNPNFKLQTFSVG
LTGAPDFKYSQIVADYIGSEHHEIHFTVEEGLDCIRDIIYHLETYDITTIRASIPMYIMM
RYIKSRGLKMVLSGEGADEILGGYLYFHSAPNAEEFHYETVSRVLHLWSNDCLRANKSSL
AWGVELRVPFLDTNFVNYSMSVRPEDRMISTDEKKIEKFILRKAFSMNYLPDEVLWRQKE
QFSDGVGYNWIDTIRTYASSHINDDEFEKAKELYPFNTPLSKEGFYYRKIFERIFPNQSC
VKTVKQWIPRQDWGCALDPSGRAQLVHVASKKINE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g9236.t1 | CDD | cd00712 | AsnB | 2 | 201 | 0.000 |
| 12 | g9236.t1 | CDD | cd01991 | Asn_Synthase_B_C | 247 | 485 | 0.000 |
| 7 | g9236.t1 | Gene3D | G3DSA:3.60.20.10 | Glutamine Phosphoribosylpyrophosphate | 32 | 224 | 0.000 |
| 8 | g9236.t1 | Gene3D | G3DSA:3.40.50.620 | HUPs | 232 | 543 | 0.000 |
| 3 | g9236.t1 | PANTHER | PTHR11772 | ASPARAGINE SYNTHETASE | 1 | 572 | 0.000 |
| 4 | g9236.t1 | PANTHER | PTHR11772:SF2 | ASPARAGINE SYNTHETASE | 1 | 572 | 0.000 |
| 10 | g9236.t1 | PIRSF | PIRSF001589 | Asn_synthetase_glu-h | 33 | 394 | 0.000 |
| 9 | g9236.t1 | PIRSF | PIRSF001589 | Asn_synthetase_glu-h | 389 | 572 | 0.000 |
| 1 | g9236.t1 | Pfam | PF13537 | Glutamine amidotransferase domain | 77 | 197 | 0.000 |
| 2 | g9236.t1 | Pfam | PF00733 | Asparagine synthase | 246 | 393 | 0.000 |
| 13 | g9236.t1 | ProSiteProfiles | PS51278 | Glutamine amidotransferase type 2 domain profile. | 2 | 217 | 25.802 |
| 6 | g9236.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 1 | 222 | 0.000 |
| 5 | g9236.t1 | SUPERFAMILY | SSF52402 | Adenine nucleotide alpha hydrolases-like | 245 | 508 | 0.000 |
| 14 | g9236.t1 | TIGRFAM | TIGR01536 | asn_synth_AEB: asparagine synthase (glutamine-hydrolyzing) | 46 | 401 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006529 | asparagine biosynthetic process | BP |
| GO:0004066 | asparagine synthase (glutamine-hydrolyzing) activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01953 (asparagine synthase (glutamine-hydrolysing))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed