General information for g9243.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 313a4.
Parent gene
- The parent gene g9243 is located on chr_1: 1112098..1115660 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9243 | g9243.t1 | TSS | g9243.t1 | 1112052 | 1112052 |
| chr_1 | g9243 | g9243.t1 | isoform | g9243.t1 | 1112098 | 1115660 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon1 | 1112098 | 1112269 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS1 | 1112098 | 1112269 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon2 | 1112354 | 1112545 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS2 | 1112354 | 1112545 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon3 | 1112802 | 1112987 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS3 | 1112802 | 1112987 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon4 | 1113089 | 1113152 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS4 | 1113089 | 1113152 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon5 | 1113433 | 1113703 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS5 | 1113433 | 1113703 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon6 | 1114822 | 1115023 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS6 | 1114822 | 1115023 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon7 | 1115096 | 1115131 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS7 | 1115096 | 1115131 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon8 | 1115247 | 1115435 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS8 | 1115247 | 1115435 |
| chr_1 | g9243 | g9243.t1 | exon | g9243.t1.exon9 | 1115488 | 1115660 |
| chr_1 | g9243 | g9243.t1 | cds | g9243.t1.CDS9 | 1115488 | 1115660 |
| chr_1 | g9243 | g9243.t1 | TTS | g9243.t1 | 1115844 | 1115844 |
Sequences
>g9243.t1 Gene=g9243 Length=1485
ATGTACGAGACTGATTTACTTTTTGGAATTGCCGTTATTCTACTTTTCATCTACCTGAGA
ACTTTTGTGCGTGACTTTCTGAAATATCATCATCAATTTTGGAAAATACCCGGACCTAAA
ACCTTACCACTAACACTCATCGCATATTTATTCACTGCTCGATCTAATGCCGATCGCTTT
GAGATTCTTCAAAATTTGAGTAAAAGATATCCGAGATATTATTCATTATGGTTTGGCACA
ATGTATCTGTTTATTACCGATGATCCTGATGTTGCACAAAAACTTTTAACACTGCCGCAA
TGTGTCGAAAAAAACTTTTTTATGGACTTGTTTGGTTTTCCAAATGGCCTGATTGCTATG
AAGGCTGACAAATGGAAAAATGAAAGAAAACTTTTGAATTCAACATTTAGTTTGCCAGTG
CTACAAAGTTATTTGCCAATTTTTACAAAATGTATTTCAAAGAGCATAGAATGTTTAAAT
GAAAAGTGCGATAAGGAAGAATTTGACATAAAACATAATGTTACTGTGGTTGTTATGAAT
GCTGTTATGAACACAACTTTCGGAAAAGACTTATCACGAGATGTTGCAGAAACATTTATG
ACAAACACCGAAAAACTTTTGGATATCATTTGGAAACGAGCAATGAAGCCACATTATCAT
TACAAATCAATTTATTCATTGTCAAATATGCAGAAAGAAGAAAATGAATACAGAAAATTT
ATTTTTGATTTTGTAACAACTGAATGGAACAAATTGAAAAACAATGACAACGATATGAAC
TCTGTTGATAATTCTTTCATACATCATTTAATGAAAGTCTCAAACAATGGACGAAATTTT
ACAAGCGAGGAAATTCTTGATCATTGTGGAACAATGCTTGTTGCTGCAGCAGACACGATG
AGTACAGCCCTAAAATTTACTCTTTTAATGCTTGCCATGCACGAAAACGTGCAGCAGCGC
ATAGTTGAGGAAATCAATGAAATATTTGGCGAAGAAGATCTTATCGTTGACTATGAAAAG
CTATCGGAACTAAAGTACATTGAGATGACTATAAAGGAAGCATTAAGACTGTTCACACCA
ACAACAGGGTTATTCCGTGAAGTAAGAGCTGATGTTAATATCGGCATCGACAATGTTTGC
TTACCAAAAGGAACAAATTTGGTGATAAATGTTTATGGATTGCATCGTAAAAAAGAACTT
TGGGGTGATGATGCTCATCTTTTTAGACCAGAACGTTTTAGTGCAGAAAATTCATTCAAT
CGACATCAATATGCGTTTTTGCCTTTTGCCAATGGTATGAGGCTATGTATAGGAAATCGA
TATGCTATGATATTTATGAAGCTAATTGTTGTTCATTATATTAAGAATTTTCATTTCTCA
ACGTCATTGAAATATGAAAATGTTCGAGTGAAGGCGGGCATCACACTGAAATTGATTGGC
AATCATTTAGTGAAAATAACAACAAGGAGGAAGGGTGACGACTAA
>g9243.t1 Gene=g9243 Length=494
MYETDLLFGIAVILLFIYLRTFVRDFLKYHHQFWKIPGPKTLPLTLIAYLFTARSNADRF
EILQNLSKRYPRYYSLWFGTMYLFITDDPDVAQKLLTLPQCVEKNFFMDLFGFPNGLIAM
KADKWKNERKLLNSTFSLPVLQSYLPIFTKCISKSIECLNEKCDKEEFDIKHNVTVVVMN
AVMNTTFGKDLSRDVAETFMTNTEKLLDIIWKRAMKPHYHYKSIYSLSNMQKEENEYRKF
IFDFVTTEWNKLKNNDNDMNSVDNSFIHHLMKVSNNGRNFTSEEILDHCGTMLVAAADTM
STALKFTLLMLAMHENVQQRIVEEINEIFGEEDLIVDYEKLSELKYIEMTIKEALRLFTP
TTGLFREVRADVNIGIDNVCLPKGTNLVINVYGLHRKKELWGDDAHLFRPERFSAENSFN
RHQYAFLPFANGMRLCIGNRYAMIFMKLIVVHYIKNFHFSTSLKYENVRVKAGITLKLIG
NHLVKITTRRKGDD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g9243.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 23 | 493 | 1.1E-95 |
| 2 | g9243.t1 | PANTHER | PTHR24291:SF140 | CYTOCHROME P450 4C3-RELATED | 11 | 477 | 1.1E-77 |
| 3 | g9243.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 11 | 477 | 1.1E-77 |
| 7 | g9243.t1 | PRINTS | PR00463 | E-class P450 group I signature | 67 | 86 | 1.4E-13 |
| 6 | g9243.t1 | PRINTS | PR00463 | E-class P450 group I signature | 177 | 195 | 1.4E-13 |
| 12 | g9243.t1 | PRINTS | PR00385 | P450 superfamily signature | 295 | 312 | 3.7E-8 |
| 8 | g9243.t1 | PRINTS | PR00463 | E-class P450 group I signature | 304 | 330 | 1.4E-13 |
| 11 | g9243.t1 | PRINTS | PR00385 | P450 superfamily signature | 349 | 360 | 3.7E-8 |
| 5 | g9243.t1 | PRINTS | PR00463 | E-class P450 group I signature | 426 | 436 | 1.4E-13 |
| 10 | g9243.t1 | PRINTS | PR00385 | P450 superfamily signature | 427 | 436 | 3.7E-8 |
| 4 | g9243.t1 | PRINTS | PR00463 | E-class P450 group I signature | 436 | 459 | 1.4E-13 |
| 9 | g9243.t1 | PRINTS | PR00385 | P450 superfamily signature | 436 | 447 | 3.7E-8 |
| 1 | g9243.t1 | Pfam | PF00067 | Cytochrome P450 | 37 | 461 | 9.0E-82 |
| 17 | g9243.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 18 | g9243.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 23 | - |
| 16 | g9243.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 24 | 494 | - |
| 14 | g9243.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 36 | 489 | 2.88E-95 |
| 13 | g9243.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 24 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed