General information for g9296.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Allatostatin-A receptor.
Parent gene
- The parent gene g9296 is located on chr_1: 1597001..1605847 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9296 | g9296.t1 | isoform | g9296.t1 | 1597001 | 1605847 |
| chr_1 | g9296 | g9296.t1 | exon | g9296.t1.exon1 | 1597001 | 1597193 |
| chr_1 | g9296 | g9296.t1 | cds | g9296.t1.CDS1 | 1597001 | 1597193 |
| chr_1 | g9296 | g9296.t1 | exon | g9296.t1.exon2 | 1597271 | 1597397 |
| chr_1 | g9296 | g9296.t1 | cds | g9296.t1.CDS2 | 1597271 | 1597397 |
| chr_1 | g9296 | g9296.t1 | exon | g9296.t1.exon3 | 1599143 | 1599301 |
| chr_1 | g9296 | g9296.t1 | cds | g9296.t1.CDS3 | 1599143 | 1599301 |
| chr_1 | g9296 | g9296.t1 | exon | g9296.t1.exon4 | 1599372 | 1599519 |
| chr_1 | g9296 | g9296.t1 | cds | g9296.t1.CDS4 | 1599372 | 1599519 |
| chr_1 | g9296 | g9296.t1 | exon | g9296.t1.exon5 | 1602542 | 1602666 |
| chr_1 | g9296 | g9296.t1 | cds | g9296.t1.CDS5 | 1602542 | 1602666 |
| chr_1 | g9296 | g9296.t1 | exon | g9296.t1.exon6 | 1603946 | 1603985 |
| chr_1 | g9296 | g9296.t1 | cds | g9296.t1.CDS6 | 1603946 | 1603985 |
| chr_1 | g9296 | g9296.t1 | exon | g9296.t1.exon7 | 1605530 | 1605847 |
| chr_1 | g9296 | g9296.t1 | cds | g9296.t1.CDS7 | 1605530 | 1605847 |
| chr_1 | g9296 | g9296.t1 | TSS | g9296.t1 | NA | NA |
| chr_1 | g9296 | g9296.t1 | TTS | g9296.t1 | NA | NA |
Sequences
>g9296.t1 Gene=g9296 Length=1110
ATGTATCCATCGTGTCTAAACATTACGGATTCACATGAGCTGAGCATATGTGAAGCGGCA
AAACAACAAAACGAAACTGAGCCAGAACCAATTTTTGATGAAAATTTGGAGCGAATTGTT
TCATTGACAGTGTTTGTGCTGTTTGGCATAATATTTGTTGTAGGATTAATTGGTAACGCT
TTAGTGGTTATCGTGGTTGCTTTTAATCCATTAATGCGATCAACAACAAACATTTTAATT
TTCAATTTAGCAATTTCGGATTTGATGTTTGTAATTATGTGTATACCATTTACGGCTAGT
GACTATATTTTAACAAATTGGCTTTTCGGAGACTTTATGTGTAAATGCGTACAATATATG
ATTGTAGTCACGTGTTTAGGCAGTGTTTATACATTAGTACTTATGTCATTAGACAGATTT
CTAGCTGTCGTTCATCCTATTTCGAGCATGTCGATACGAACAGAAAAAAATGCCTTAATA
GCGATAATCCTAGCATGGTTTTGTATATTTTCTGCTGCAATACCAACCGCAATAAGCCAT
GGCGAGGTCGAGTATGTGCATAAAGGTGAAAATCACACAGCATGTCTTTTTCTATCAGAG
CAAGGCTACCATCATGACTATTTTCAGATATCGTTTTTTTTATCATCATATGTCATTCCA
TTGTCGTTAATATCATTCCTTTATGTCGGCATGTTGGCTCGACTTTGGAGTTCAAATGCA
CCGGGTGGTGGAAAAGGCTCAAAAGAATCCAGGAAAGGCAAGAAGAGAGTCACGAGATTA
GTTGTTTTTGTGGTCGTAGCATTTCTCATATGTTGGGCACCAATTCAAATAGTTCTATTA
CTAAAATCGTTAAATCTTTATAAAACAAATGCAATAACCGTCTCGATTCAGATCATTAGT
CATGTGCTGGCATATTTAAATTCATGTCTAAATTTTATCCTGTATGCCTTTCTAAGTGAG
AATTTTAGGAAAGCATTTAGAAAGGTAATGTGGTGTGGTCATCAACGGCAGTCTTCGTCA
CATTATGTCCTTACTGCACCTGACACCAAATCTACACGCACAAGAACAGCAAATACGGGA
AATGGTGCGGCAAGTGTCGACATTCTATAA
>g9296.t1 Gene=g9296 Length=369
MYPSCLNITDSHELSICEAAKQQNETEPEPIFDENLERIVSLTVFVLFGIIFVVGLIGNA
LVVIVVAFNPLMRSTTNILIFNLAISDLMFVIMCIPFTASDYILTNWLFGDFMCKCVQYM
IVVTCLGSVYTLVLMSLDRFLAVVHPISSMSIRTEKNALIAIILAWFCIFSAAIPTAISH
GEVEYVHKGENHTACLFLSEQGYHHDYFQISFFLSSYVIPLSLISFLYVGMLARLWSSNA
PGGGKGSKESRKGKKRVTRLVVFVVVAFLICWAPIQIVLLLKSLNLYKTNAITVSIQIIS
HVLAYLNSCLNFILYAFLSENFRKAFRKVMWCGHQRQSSSHYVLTAPDTKSTRTRTANTG
NGAASVDIL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 33 | g9296.t1 | CDD | cd15096 | 7tmA_AstA_R_insect | 42 | 326 | 1.01545E-132 |
| 17 | g9296.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 11 | 365 | 1.6E-89 |
| 2 | g9296.t1 | PANTHER | PTHR45695:SF12 | NEUROPEPTIDE CCHAMIDE-1 RECEPTOR | 33 | 356 | 6.3E-76 |
| 3 | g9296.t1 | PANTHER | PTHR45695 | LEUCOKININ RECEPTOR-RELATED | 33 | 356 | 6.3E-76 |
| 8 | g9296.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 43 | 67 | 5.8E-48 |
| 10 | g9296.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 76 | 97 | 5.8E-48 |
| 15 | g9296.t1 | PRINTS | PR00663 | Galanin receptor signature | 90 | 104 | 1.1E-15 |
| 12 | g9296.t1 | PRINTS | PR00663 | Galanin receptor signature | 107 | 125 | 1.1E-15 |
| 9 | g9296.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 121 | 143 | 5.8E-48 |
| 14 | g9296.t1 | PRINTS | PR00663 | Galanin receptor signature | 149 | 165 | 1.1E-15 |
| 7 | g9296.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 157 | 178 | 5.8E-48 |
| 11 | g9296.t1 | PRINTS | PR00663 | Galanin receptor signature | 204 | 219 | 1.1E-15 |
| 4 | g9296.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 209 | 232 | 5.8E-48 |
| 5 | g9296.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 257 | 281 | 5.8E-48 |
| 13 | g9296.t1 | PRINTS | PR00663 | Galanin receptor signature | 284 | 304 | 1.1E-15 |
| 6 | g9296.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 297 | 323 | 5.8E-48 |
| 1 | g9296.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 58 | 315 | 2.4E-56 |
| 25 | g9296.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 38 | - |
| 32 | g9296.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 39 | 67 | - |
| 18 | g9296.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 68 | 78 | - |
| 29 | g9296.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 79 | 99 | - |
| 23 | g9296.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 100 | 118 | - |
| 31 | g9296.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 119 | 137 | - |
| 19 | g9296.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 138 | 157 | - |
| 30 | g9296.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 158 | 178 | - |
| 24 | g9296.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 179 | 216 | - |
| 28 | g9296.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 217 | 236 | - |
| 20 | g9296.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 237 | 256 | - |
| 27 | g9296.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 257 | 278 | - |
| 22 | g9296.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 279 | 297 | - |
| 26 | g9296.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 298 | 318 | - |
| 21 | g9296.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 319 | 369 | - |
| 42 | g9296.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 127 | 143 | - |
| 43 | g9296.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 58 | 315 | 47.433 |
| 41 | g9296.t1 | SMART | SM01381 | 7TM_GPCR_Srsx_2 | 52 | 330 | 8.4E-7 |
| 16 | g9296.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 38 | 347 | 4.67E-79 |
| 40 | g9296.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 44 | 66 | - |
| 39 | g9296.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 78 | 100 | - |
| 38 | g9296.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 120 | 137 | - |
| 35 | g9296.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 157 | 179 | - |
| 36 | g9296.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 207 | 229 | - |
| 37 | g9296.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 259 | 281 | - |
| 34 | g9296.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 296 | 318 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04230 (galanin receptor 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed