General information for g935.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Glutathione-specific gamma-glutamylcyclotransferase 1.
Parent gene
- The parent gene g935 is located on chr_3: 6974822..6976564 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g935 | g935.t1 | TSS | g935.t1 | 6971698 | 6971698 |
| chr_3 | g935 | g935.t1 | isoform | g935.t1 | 6972098 | 6974822 |
| chr_3 | g935 | g935.t1 | exon | g935.t1.exon1 | 6972098 | 6972189 |
| chr_3 | g935 | g935.t1 | cds | g935.t1.CDS1 | 6972098 | 6972189 |
| chr_3 | g935 | g935.t1 | exon | g935.t1.exon2 | 6972280 | 6972400 |
| chr_3 | g935 | g935.t1 | cds | g935.t1.CDS2 | 6972280 | 6972400 |
| chr_3 | g935 | g935.t1 | exon | g935.t1.exon3 | 6974271 | 6974822 |
| chr_3 | g935 | g935.t1 | cds | g935.t1.CDS3 | 6974271 | 6974822 |
| chr_3 | g935 | g935.t1 | TTS | g935.t1 | 6974920 | 6974920 |
Sequences
>g935.t1 Gene=g935 Length=765
ATGGAAGACGCATTCAATATATTCCGAGATGATGATTATGACAGTGATAGCTCAGAATCA
TCAAATACCGAACCTACATGCTATGTTTTTGGGTATGGTTCACTTGTGTGGCGACCGGGT
TTTTCCTTTACTGAATGTATCACTGGATATATTCGTGGATATAAGAGAAGATTCTGGCAA
GGGAATACTACGCATCGTGGAACATACGAAAAGCCCGGTAGAGTAGTGACACTCGTTGAA
GATGAAAATGGTATCGTGTGGGGATGTGCATACAAAGTAACAGGCGAGCTTGCATTAAAA
TACCTCGAACAAAGGGAATGTAAATTAGGAGGATATGACGTTAAATATATCAAGTTTTTT
CCCGGAAAAACGTCTGAATTTTCTGGAATATCGGGAGAAGCTTTTCCAGCGATAATTTAT
ATAGCTACACCTGATAATTCATATTGGATGGGAGAAGACAGTTTAGAAAATATTGCGAAA
CAAATTTGTTCTGCCAGTGGACCAAGCGGTCATAATGTTGAGTATTTATTAAGATTGGCT
TTATTTATGAGAGAAGAAGTTCCTCATGCTGATGATGATCATTTGTTCACATTAGAAAAA
TTAGTAAAAGAGGAACTGATAAAACGTAAGATATGTCTTTATTCTGTGATGGGTCAACAG
CCACAAATGATTGAAAGAGATTATCATGAAAACATTAGACGACCTGTTACATTTGCACAC
TCATCGAAAATTGCTGAAAAGAAACTGCGCTGTCTCAATATTTGA
>g935.t1 Gene=g935 Length=254
MEDAFNIFRDDDYDSDSSESSNTEPTCYVFGYGSLVWRPGFSFTECITGYIRGYKRRFWQ
GNTTHRGTYEKPGRVVTLVEDENGIVWGCAYKVTGELALKYLEQRECKLGGYDVKYIKFF
PGKTSEFSGISGEAFPAIIYIATPDNSYWMGEDSLENIAKQICSASGPSGHNVEYLLRLA
LFMREEVPHADDDHLFTLEKLVKEELIKRKICLYSVMGQQPQMIERDYHENIRRPVTFAH
SSKIAEKKLRCLNI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g935.t1 | CDD | cd06661 | GGCT_like | 29 | 142 | 0 |
| 5 | g935.t1 | Gene3D | G3DSA:3.10.490.10 | Hypothetical upf0131 protein ytfp | 14 | 196 | 0 |
| 2 | g935.t1 | PANTHER | PTHR12192:SF2 | GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 1 | 20 | 210 | 0 |
| 3 | g935.t1 | PANTHER | PTHR12192 | CATION TRANSPORT PROTEIN CHAC-RELATED | 20 | 210 | 0 |
| 1 | g935.t1 | Pfam | PF04752 | ChaC-like protein | 27 | 204 | 0 |
| 4 | g935.t1 | SUPERFAMILY | SSF110857 | Gamma-glutamyl cyclotransferase-like | 25 | 152 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003839 | gamma-glutamylcyclotransferase activity | MF |
| GO:0006751 | glutathione catabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K07232 (glutathione-specific gamma-glutamylcyclotransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
