General information for g9352.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Trypsin-7.
Parent gene
- The parent gene g9352 is located on chr_1: 2179758..2181103 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9352 | g9352.t3 | TSS | g9352.t3 | 2179734 | 2179734 |
| chr_1 | g9352 | g9352.t3 | isoform | g9352.t3 | 2179758 | 2181103 |
| chr_1 | g9352 | g9352.t3 | exon | g9352.t3.exon1 | 2179758 | 2179905 |
| chr_1 | g9352 | g9352.t3 | cds | g9352.t3.CDS1 | 2179758 | 2179905 |
| chr_1 | g9352 | g9352.t3 | exon | g9352.t3.exon2 | 2179984 | 2180025 |
| chr_1 | g9352 | g9352.t3 | cds | g9352.t3.CDS2 | 2179984 | 2180025 |
| chr_1 | g9352 | g9352.t3 | exon | g9352.t3.exon3 | 2180094 | 2180340 |
| chr_1 | g9352 | g9352.t3 | cds | g9352.t3.CDS3 | 2180094 | 2180340 |
| chr_1 | g9352 | g9352.t3 | exon | g9352.t3.exon4 | 2180708 | 2180865 |
| chr_1 | g9352 | g9352.t3 | cds | g9352.t3.CDS4 | 2180708 | 2180865 |
| chr_1 | g9352 | g9352.t3 | exon | g9352.t3.exon5 | 2180937 | 2181103 |
| chr_1 | g9352 | g9352.t3 | cds | g9352.t3.CDS5 | 2180937 | 2181103 |
| chr_1 | g9352 | g9352.t3 | TTS | g9352.t3 | 2181156 | 2181156 |
Sequences
>g9352.t3 Gene=g9352 Length=762
ATGAAAATATTCTTAATTTTTGTGAGCTTAATTGCTTCCATTATTGCGGCAAATGTCCGT
AGTGGTGGTAAAATAGTTGGTGGAATTGAAGTGCCAATTGAAAATGTGCCATATCAAGTT
TCTGTCCGGTATAACAACTACCATACTTGCTCGATTATATCTTCATCCTTCATATTGACA
GCACAACATTGCACTTCAGGAAGAACTGCTGGATATTTAACAGTTCGTGCTGGTTCTAAC
AGTAGCATCAATGGTGGTGAAATTTTCCAAGTATCAAAAATCATTCCACATCCAAATTTC
AATGAATATTCACTCGATTATGACTTTTCATTGCTTAAATTGACCAATGCTATTGTACTT
GATGGAATTACAAAAGACAAAGTTCAATTGCCAACACAAGATGAAATTTTAGCTAAAGGA
ATCGCAATGTTTGTGAGCGGCTGGGGAGCAACACTTAATCCAAACCAATCAACCAGTATT
TTACGAGGTGTCACAATCACCAGCATCGACTTTGACACGTGCAATTTAATATACTTGAGA
GATGGTGGCTTGGGTGCATCACAGATATGTGCGGGTGAATTGGCCGGCGGAAAGGACTCG
TGTTACGGGGACTCAGGCGGGCCAATGGTGTCATTGGCTGGTACGAGAAAACTTTATGGC
GTTGTGTCATGGGGCATAGATTGTGCATTGCCTGGCTGGCCAGGTGTCTATGCAAGAGTT
TCACTCGTTCGTAATTGGATTGATGAAAATACCAAAGTTTAA
>g9352.t3 Gene=g9352 Length=253
MKIFLIFVSLIASIIAANVRSGGKIVGGIEVPIENVPYQVSVRYNNYHTCSIISSSFILT
AQHCTSGRTAGYLTVRAGSNSSINGGEIFQVSKIIPHPNFNEYSLDYDFSLLKLTNAIVL
DGITKDKVQLPTQDEILAKGIAMFVSGWGATLNPNQSTSILRGVTITSIDFDTCNLIYLR
DGGLGASQICAGELAGGKDSCYGDSGGPMVSLAGTRKLYGVVSWGIDCALPGWPGVYARV
SLVRNWIDENTKV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g9352.t3 | CDD | cd00190 | Tryp_SPc | 25 | 250 | 4.5643E-80 |
| 9 | g9352.t3 | Gene3D | G3DSA:2.40.10.10 | - | 17 | 253 | 2.0E-57 |
| 2 | g9352.t3 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 17 | 251 | 1.2E-73 |
| 3 | g9352.t3 | PANTHER | PTHR24276:SF83 | GH13245P2-RELATED | 17 | 251 | 1.2E-73 |
| 6 | g9352.t3 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 49 | 64 | 8.9E-11 |
| 4 | g9352.t3 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 104 | 118 | 8.9E-11 |
| 5 | g9352.t3 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 198 | 210 | 8.9E-11 |
| 1 | g9352.t3 | Pfam | PF00089 | Trypsin | 25 | 247 | 6.9E-47 |
| 11 | g9352.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 12 | g9352.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 13 | g9352.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 14 | g9352.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 10 | g9352.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 253 | - |
| 18 | g9352.t3 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 199 | 210 | - |
| 19 | g9352.t3 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 25 | 252 | 28.282 |
| 17 | g9352.t3 | SMART | SM00020 | trypsin_2 | 24 | 247 | 1.0E-67 |
| 7 | g9352.t3 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 18 | 252 | 2.74E-66 |
| 8 | g9352.t3 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
| 16 | g9352.t3 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed