General information for g9361.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4d2.
Parent gene
- The parent gene g9361 is located on chr_1: 2256135..2256811 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9361 | g9361.t1 | TTS | g9361.t1 | 2254813 | 2254813 |
| chr_1 | g9361 | g9361.t1 | isoform | g9361.t1 | 2254989 | 2256811 |
| chr_1 | g9361 | g9361.t1 | exon | g9361.t1.exon1 | 2254989 | 2255161 |
| chr_1 | g9361 | g9361.t1 | cds | g9361.t1.CDS1 | 2254989 | 2255161 |
| chr_1 | g9361 | g9361.t1 | exon | g9361.t1.exon2 | 2255222 | 2255414 |
| chr_1 | g9361 | g9361.t1 | cds | g9361.t1.CDS2 | 2255222 | 2255414 |
| chr_1 | g9361 | g9361.t1 | exon | g9361.t1.exon3 | 2255473 | 2255995 |
| chr_1 | g9361 | g9361.t1 | cds | g9361.t1.CDS3 | 2255473 | 2255995 |
| chr_1 | g9361 | g9361.t1 | exon | g9361.t1.exon4 | 2256125 | 2256575 |
| chr_1 | g9361 | g9361.t1 | cds | g9361.t1.CDS4 | 2256125 | 2256575 |
| chr_1 | g9361 | g9361.t1 | exon | g9361.t1.exon5 | 2256646 | 2256811 |
| chr_1 | g9361 | g9361.t1 | cds | g9361.t1.CDS5 | 2256646 | 2256811 |
| chr_1 | g9361 | g9361.t1 | TSS | g9361.t1 | 2256887 | 2256887 |
Sequences
>g9361.t1 Gene=g9361 Length=1506
ATGATAATTATATGGTTTACTATTTCGGTCATTGTTTTACTTTATCTATGGGACAAAAAT
GGTTTTAAAAATCCAAGATTTATTAAATGTTCTGAAAAGTTCAACGGTCCTAAAACTATT
CCTATTTTTGGAAATGCATTGCAATATTTAGCAAAATCAACTGAAGATGCGCTTCCATTA
GCTGCAAAATTTTTCAATATTTATGGTGATACTGTGAGAATTTGGGCATTTAAAAGATTT
GTTCTTTTAACTAAAGATCCAAAATTTTTCGAAGTTATTCTATCAAGTCAAAAGCAATTA
ACTAAAAATAATCTTTATGATTTGATGCACGACTGGCTTGGTGGTGGACTTTTGCTTTCA
ACAAATGAAAAATGGTTTCAAAGAAGAAAAATTATAACTCCAACATTTCATTTTAAAATA
TTGCAAGAATTTGTCGATGTTTTCAATTATCAGAATAATATTTTTGTTAAAAAACTGAAA
GAACTAAAGAGTGAAAATCCATTTGATATTTATAACATGGTTACATTAATGTCACTCGAT
ATCATTAGTCAAACAGCAATGGGTGTTGAAATTCATGCACAAAATGATGATTCTGAATAT
GCACAAACTGTTAAAGAAATGACATACATAATTATCGATCGAATGATGCATATTTGGAAA
AGACCAAATTTTATTTATCAATTTACATCTGATAAAGTAAGATATGATAGGTGTTTAAAA
ACTTTGCATAGTTTTACAAGAAAAATTATTGAAAAACGACGCGAAACGATAATAAATGAG
GAAGATGAAGGTTTTGAAAATGTCGATGATGATTCAATAGGAATAAAGAAAAGAATGGCA
TTACTTGATGTTCTATTAAAATCGACAGTTAATGGAAAACCTTTGACAAATTCTGAGATT
GCTGAAGAGGTTGATACTTTTATGTTTGAGGGGCATGATACTGTTACAACCAGCACCACT
TTCACTCTTTATTTGCTTTCACAACATCCTGATGTTCAAAAAAAAGTCTATGAAGAAGCT
ATTAAAATTGTAGGATATGATTTAAGTGTTAATCCAACATATAATCAACTACAAGATATG
AAATATCTTGAATGTGTCATTAAAGAATCATTAAGAATGTATCCACCAGTTCCTATCATT
GGTCGAAAATTAGATGAAGACATGATTTTTGGCCAAACATTGATTCCAGCTGGTGTAAAT
ACAAACTTAATGATTTACCACTTGAACATGGATGAGAGATATTTTAAAAATCCTGAAAAA
TTCATTCCTGAAAGATTTAGTGAAACAAATATTAGAAATGAAAATCCATTTTTATATGTA
CCATTTTCCGCTGGTCCTCGAAACTGCATTGGCCAAAAATTCGCCATGTTGGAGCTTAAA
AGTACACTTTCTAATGTGATTAGAAACTTTGAATTGATTAAGAGTGACATTCCATTAAAT
ATCATTGCACAACTTACATTAAAATCTACTACTGGTGTTCATGTTGGTTTTAGACAAAGA
AAATAA
>g9361.t1 Gene=g9361 Length=501
MIIIWFTISVIVLLYLWDKNGFKNPRFIKCSEKFNGPKTIPIFGNALQYLAKSTEDALPL
AAKFFNIYGDTVRIWAFKRFVLLTKDPKFFEVILSSQKQLTKNNLYDLMHDWLGGGLLLS
TNEKWFQRRKIITPTFHFKILQEFVDVFNYQNNIFVKKLKELKSENPFDIYNMVTLMSLD
IISQTAMGVEIHAQNDDSEYAQTVKEMTYIIIDRMMHIWKRPNFIYQFTSDKVRYDRCLK
TLHSFTRKIIEKRRETIINEEDEGFENVDDDSIGIKKRMALLDVLLKSTVNGKPLTNSEI
AEEVDTFMFEGHDTVTTSTTFTLYLLSQHPDVQKKVYEEAIKIVGYDLSVNPTYNQLQDM
KYLECVIKESLRMYPPVPIIGRKLDEDMIFGQTLIPAGVNTNLMIYHLNMDERYFKNPEK
FIPERFSETNIRNENPFLYVPFSAGPRNCIGQKFAMLELKSTLSNVIRNFELIKSDIPLN
IIAQLTLKSTTGVHVGFRQRK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g9361.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 22 | 501 | 4.7E-121 |
| 2 | g9361.t1 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 9 | 500 | 3.1E-158 |
| 3 | g9361.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 9 | 500 | 3.1E-158 |
| 8 | g9361.t1 | PRINTS | PR00463 | E-class P450 group I signature | 299 | 316 | 2.4E-23 |
| 11 | g9361.t1 | PRINTS | PR00385 | P450 superfamily signature | 310 | 327 | 1.6E-12 |
| 7 | g9361.t1 | PRINTS | PR00463 | E-class P450 group I signature | 319 | 345 | 2.4E-23 |
| 5 | g9361.t1 | PRINTS | PR00463 | E-class P450 group I signature | 364 | 382 | 2.4E-23 |
| 10 | g9361.t1 | PRINTS | PR00385 | P450 superfamily signature | 365 | 376 | 1.6E-12 |
| 4 | g9361.t1 | PRINTS | PR00463 | E-class P450 group I signature | 404 | 428 | 2.4E-23 |
| 9 | g9361.t1 | PRINTS | PR00463 | E-class P450 group I signature | 439 | 449 | 2.4E-23 |
| 12 | g9361.t1 | PRINTS | PR00385 | P450 superfamily signature | 440 | 449 | 1.6E-12 |
| 6 | g9361.t1 | PRINTS | PR00463 | E-class P450 group I signature | 449 | 472 | 2.4E-23 |
| 13 | g9361.t1 | PRINTS | PR00385 | P450 superfamily signature | 449 | 460 | 1.6E-12 |
| 1 | g9361.t1 | Pfam | PF00067 | Cytochrome P450 | 36 | 473 | 2.4E-106 |
| 18 | g9361.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 19 | g9361.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 20 | g9361.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 21 | g9361.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 18 | - |
| 17 | g9361.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 501 | - |
| 22 | g9361.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 442 | 451 | - |
| 14 | g9361.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 36 | 500 | 2.36E-114 |
| 15 | g9361.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K15001 (cytochrome P450 family 4 [EC:1.14.-.-])
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed