General information for g9387.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 9f2.
Parent gene
- The parent gene g9387 is located on chr_1: 2429662..2432305 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9387 | g9387.t1 | TTS | g9387.t1 | 2429544 | 2429544 |
| chr_1 | g9387 | g9387.t1 | isoform | g9387.t1 | 2429662 | 2432305 |
| chr_1 | g9387 | g9387.t1 | exon | g9387.t1.exon1 | 2429662 | 2429836 |
| chr_1 | g9387 | g9387.t1 | cds | g9387.t1.CDS1 | 2429662 | 2429836 |
| chr_1 | g9387 | g9387.t1 | exon | g9387.t1.exon2 | 2429932 | 2429938 |
| chr_1 | g9387 | g9387.t1 | cds | g9387.t1.CDS2 | 2429932 | 2429938 |
| chr_1 | g9387 | g9387.t1 | exon | g9387.t1.exon3 | 2430014 | 2430117 |
| chr_1 | g9387 | g9387.t1 | cds | g9387.t1.CDS3 | 2430014 | 2430117 |
| chr_1 | g9387 | g9387.t1 | exon | g9387.t1.exon4 | 2430323 | 2430633 |
| chr_1 | g9387 | g9387.t1 | cds | g9387.t1.CDS4 | 2430323 | 2430633 |
| chr_1 | g9387 | g9387.t1 | exon | g9387.t1.exon5 | 2430693 | 2431291 |
| chr_1 | g9387 | g9387.t1 | cds | g9387.t1.CDS5 | 2430693 | 2431291 |
| chr_1 | g9387 | g9387.t1 | exon | g9387.t1.exon6 | 2431716 | 2431894 |
| chr_1 | g9387 | g9387.t1 | cds | g9387.t1.CDS6 | 2431716 | 2431894 |
| chr_1 | g9387 | g9387.t1 | exon | g9387.t1.exon7 | 2432097 | 2432305 |
| chr_1 | g9387 | g9387.t1 | cds | g9387.t1.CDS7 | 2432097 | 2432305 |
| chr_1 | g9387 | g9387.t1 | TSS | g9387.t1 | 2432336 | 2432336 |
Sequences
>g9387.t1 Gene=g9387 Length=1584
ATGAGTTTTGTAATTTACTTAATTGCTGCAGCAGCAACTTTTTTTGTATACAAAATTTTC
TTTCATAATAAAAATAAAAATTATTTTGCCCATAAAGGAATAAAATATGAAAAACCAAGA
GGATTATTGGCAATTATTGATATGTTTACTAAGAAAAAATCATTGCCTGATGTTGTCATG
GGATGGTACAATGATTTTAAATCGGAAAAAATTTCGGGAATTTTTGAATTTTCCCGTCCA
GTTTTTATGGTTCGCGATCCAAAATTGATAAAACAAATGGCAGTCAAAGATTTTGATTAT
TTTATGGATCATCGTGTAGTTTTGACTGAAGAAATTGATGAACTGTTTGGAAAGTCTTTA
ATTTCATTGCAAGGACAAAAATGGAAAGACATGCGTTCAACACTTTCACCAGCATTTACC
GGTCACAAAATGCGATTAATGTTTGATTTTGTAGCGAGTGTTGGTAAACAAACTGTCGAA
ACATTGAAGTGTCAAATTGATGAAGGTCGTGAAAAAGATTTTGAATTTAAAGAACTCGCA
ACAAAATTCACGGTCGATAATATTGCAAGTTGTGCTTTCGGAATTCATGTTAATAGCTTT
GCTGACCCTAAAAATGATTTTCATCGAATTGCATCAGAATTAACAAGTTCTGAAAGTTTA
ACCGTCATGCTTAAATTTACTGGTCTTTTACTTTTCCCTTGGCTTATGAAAGCTTTTAAA
ATTAGTTTTTTTAGTGAAAATATCATGAAATTCTTTAAAGAAGCAATTTTGGATACAATG
AAAACACGAGAAGAAAAAGGAATTGTCCGACATGACATGATTAATCTTCTGATGCAAGCA
AAAAAAGGAAATTTAACTCATGAAAATGAAGAAAAAACTGTTGAAGGTTTCGCAACTGTT
GAAGAATCACAGATGGGTAAAATGCAAAATAAAAGAAAATGGGACGATACAGATTTGGCT
GCACAAGCTTTGGTTTTCTTTATTGCTGGTTTTGAAACTGTTGCAACAACAATGGCTTTT
ATGGGTTATGAACTTGCAATAAATCCAGAAATTCAAAAAAAACTTTATGAAGAAATTCTT
GAAACTGAAAAAGAATTGAATGGAAAACCATTGACATATGAAAAAATTCAGACAATGAAA
TTTATGGATCAGGTTGTAAGTGAAGTTTTAAGAAAATGGCCACCATCGCCTGTTACTGAC
AGAATTTGTGTCAAAGATTATGTATTGGACTATGAAGGGAGAAAAATTACAATTGAAAAA
GACAGAAGTTTCATGATTCCTATTTGGGCATTTCATCGTGATCCAAATTATTACCGAAAT
CCTGAAAAATTTGACCCAGAAAGATTCAATGATGAAAATAGAAAAAATATTCAAGAATAC
ACATACATGCCTTTTGGTGTTGGTCCAAGAAATTGTATTGGAAGTCGTTTCGCACTGATG
GAAGTGAAAACAATTTTCTATTATTTGTTGCTTAACTTCAGAATTGCAAAAACTGATAAA
ACAAAAATTCCTCTTCAATTTAAGAGCATGTCTGTAGGTTTAAAGGTCAAAGAAGGAATT
TGGGTAGCGCTTGAACCTAAATAA
>g9387.t1 Gene=g9387 Length=527
MSFVIYLIAAAATFFVYKIFFHNKNKNYFAHKGIKYEKPRGLLAIIDMFTKKKSLPDVVM
GWYNDFKSEKISGIFEFSRPVFMVRDPKLIKQMAVKDFDYFMDHRVVLTEEIDELFGKSL
ISLQGQKWKDMRSTLSPAFTGHKMRLMFDFVASVGKQTVETLKCQIDEGREKDFEFKELA
TKFTVDNIASCAFGIHVNSFADPKNDFHRIASELTSSESLTVMLKFTGLLLFPWLMKAFK
ISFFSENIMKFFKEAILDTMKTREEKGIVRHDMINLLMQAKKGNLTHENEEKTVEGFATV
EESQMGKMQNKRKWDDTDLAAQALVFFIAGFETVATTMAFMGYELAINPEIQKKLYEEIL
ETEKELNGKPLTYEKIQTMKFMDQVVSEVLRKWPPSPVTDRICVKDYVLDYEGRKITIEK
DRSFMIPIWAFHRDPNYYRNPEKFDPERFNDENRKNIQEYTYMPFGVGPRNCIGSRFALM
EVKTIFYYLLLNFRIAKTDKTKIPLQFKSMSVGLKVKEGIWVALEPK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g9387.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 527 | 8.7E-126 |
| 2 | g9387.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 4 | 510 | 5.7E-190 |
| 3 | g9387.t1 | PANTHER | PTHR24292:SF90 | CYTOCHROME P450 317A1-RELATED | 4 | 510 | 5.7E-190 |
| 5 | g9387.t1 | PRINTS | PR00463 | E-class P450 group I signature | 318 | 335 | 4.1E-19 |
| 10 | g9387.t1 | PRINTS | PR00385 | P450 superfamily signature | 329 | 346 | 4.4E-11 |
| 7 | g9387.t1 | PRINTS | PR00463 | E-class P450 group I signature | 338 | 364 | 4.1E-19 |
| 11 | g9387.t1 | PRINTS | PR00385 | P450 superfamily signature | 384 | 395 | 4.4E-11 |
| 4 | g9387.t1 | PRINTS | PR00463 | E-class P450 group I signature | 427 | 451 | 4.1E-19 |
| 8 | g9387.t1 | PRINTS | PR00463 | E-class P450 group I signature | 462 | 472 | 4.1E-19 |
| 9 | g9387.t1 | PRINTS | PR00385 | P450 superfamily signature | 463 | 472 | 4.4E-11 |
| 6 | g9387.t1 | PRINTS | PR00463 | E-class P450 group I signature | 472 | 495 | 4.1E-19 |
| 12 | g9387.t1 | PRINTS | PR00385 | P450 superfamily signature | 472 | 483 | 4.4E-11 |
| 1 | g9387.t1 | Pfam | PF00067 | Cytochrome P450 | 69 | 518 | 1.1E-83 |
| 16 | g9387.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 17 | g9387.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 18 | g9387.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 19 | g9387.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 18 | - |
| 15 | g9387.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 527 | - |
| 22 | g9387.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 465 | 474 | - |
| 13 | g9387.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 47 | 526 | 1.31E-105 |
| 21 | g9387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 4 | 21 | - |
| 20 | g9387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 319 | 341 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed