General information for g9415.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 CYP12A2.
Parent gene
- The parent gene g9415 is located on chr_1: 2800664..2802033 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9415 | g9415.t1 | isoform | g9415.t1 | 2800664 | 2802033 |
| chr_1 | g9415 | g9415.t1 | exon | g9415.t1.exon1 | 2800664 | 2800734 |
| chr_1 | g9415 | g9415.t1 | cds | g9415.t1.CDS1 | 2800664 | 2800734 |
| chr_1 | g9415 | g9415.t1 | exon | g9415.t1.exon2 | 2800793 | 2801068 |
| chr_1 | g9415 | g9415.t1 | cds | g9415.t1.CDS2 | 2800793 | 2801068 |
| chr_1 | g9415 | g9415.t1 | exon | g9415.t1.exon3 | 2801314 | 2801464 |
| chr_1 | g9415 | g9415.t1 | cds | g9415.t1.CDS3 | 2801314 | 2801464 |
| chr_1 | g9415 | g9415.t1 | exon | g9415.t1.exon4 | 2801518 | 2802033 |
| chr_1 | g9415 | g9415.t1 | cds | g9415.t1.CDS4 | 2801518 | 2802033 |
| chr_1 | g9415 | g9415.t1 | TSS | g9415.t1 | NA | NA |
| chr_1 | g9415 | g9415.t1 | TTS | g9415.t1 | NA | NA |
Sequences
>g9415.t1 Gene=g9415 Length=1014
ATGATGCAACCAAAAATTGTCAACATTTATACACAACAATTTGATGAAATTGCCAAAGAA
TTTATTCAAATCATGAAAAAGTTGAAAAATAATAGAAGTGAAATGCCTAAAGATTTTTTT
CAATACCTCACAAAATGGTCACTCGAAACTATTGCAACAGTTTCAATTGAAAAGCGATTG
AATATTTTTAATGGAAAAACCGATGATGAAAGAAGTAAAAAATACATGGAAGTTTCTGCA
CAATTTTTTGATAAATTGACGGACTTTGAATTGATGCCATCAATTTGGAAATATTATCAA
ACAAAATATTTTAAGGAACTTATGAATGTTTATGACACAATGGCTAAAATTACATTCAGT
TACATCGAAGAAGCCATAAAAAAGTTTGAAACAGAAAGGTTCAAGGAAGTTCAAGAGGAG
AGCATTTTGAGAAAACTCTTGAAAATAAACAAACAAATTGCATTTGTTATTGCTGGTGAT
ATATTAATGCCTGATATGGTAGCTGCAGCAACAAATTTTACCCTCTACTTCCTAGCAAAA
AATTTAGAAAAGCAGGAAAAATTACGATCTGAAGTGATGAAAATTTTACCAAATAAAAAT
TCATCCATAGAACCAAAAAGTTTACAGAATATGCCATACTTAAAAGCTGTAATTAAAGAA
AGCTTGCGATTACAACCTATTGTCAGCGGAAATTCAAGAGAACTTGCTAAAGACGTCGTA
CTTGCAGGTTATCATGTGCCAAAAGGAACAATGATTTTCATGAACGCTTACATTGAATTG
TCAAATCCAAAATATTATCCAAATCCAGAAAAATTCATTCCTGAGAGATGGTTAAGAGAC
CAACAAAATGAGAATTGGAAATCAAAAGAATACAACGCATTTTCATATTTACTTTTTGGA
TTTGGTTCAAGGCAAGATCTTAAAAGTTTTAAAAAAGATTTTTTAATAAAATTTGTATTT
TTTGTAGATCTTGCATTGGAAAACGTTTTGCTGAACTTCAAATGGAAATTTTAA
>g9415.t1 Gene=g9415 Length=337
MMQPKIVNIYTQQFDEIAKEFIQIMKKLKNNRSEMPKDFFQYLTKWSLETIATVSIEKRL
NIFNGKTDDERSKKYMEVSAQFFDKLTDFELMPSIWKYYQTKYFKELMNVYDTMAKITFS
YIEEAIKKFETERFKEVQEESILRKLLKINKQIAFVIAGDILMPDMVAAATNFTLYFLAK
NLEKQEKLRSEVMKILPNKNSSIEPKSLQNMPYLKAVIKESLRLQPIVSGNSRELAKDVV
LAGYHVPKGTMIFMNAYIELSNPKYYPNPEKFIPERWLRDQQNENWKSKEYNAFSYLLFG
FGSRQDLKSFKKDFLIKFVFFVDLALENVLLNFKWKF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g9415.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 305 | 6.7E-72 |
| 2 | g9415.t1 | PANTHER | PTHR24291:SF155 | CYTOCHROME P450 301B1 | 1 | 304 | 3.1E-75 |
| 3 | g9415.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 1 | 304 | 3.1E-75 |
| 5 | g9415.t1 | PRINTS | PR00465 | E-class P450 group IV signature | 211 | 227 | 4.7E-9 |
| 4 | g9415.t1 | PRINTS | PR00465 | E-class P450 group IV signature | 244 | 258 | 4.7E-9 |
| 6 | g9415.t1 | PRINTS | PR00465 | E-class P450 group IV signature | 260 | 278 | 4.7E-9 |
| 1 | g9415.t1 | Pfam | PF00067 | Cytochrome P450 | 7 | 305 | 3.6E-49 |
| 11 | g9415.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 152 | - |
| 12 | g9415.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 153 | 179 | - |
| 9 | g9415.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 180 | 313 | - |
| 13 | g9415.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 314 | 333 | - |
| 10 | g9415.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 334 | 337 | - |
| 7 | g9415.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 3 | 304 | 1.26E-56 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed