General information for g9416.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g9416 is located on chr_1: 2807988..2811206 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9416 | g9416.t1 | isoform | g9416.t1 | 2807988 | 2811206 |
| chr_1 | g9416 | g9416.t1 | exon | g9416.t1.exon1 | 2807988 | 2808433 |
| chr_1 | g9416 | g9416.t1 | cds | g9416.t1.CDS1 | 2807988 | 2808433 |
| chr_1 | g9416 | g9416.t1 | exon | g9416.t1.exon2 | 2808568 | 2809873 |
| chr_1 | g9416 | g9416.t1 | cds | g9416.t1.CDS2 | 2808568 | 2809873 |
| chr_1 | g9416 | g9416.t1 | exon | g9416.t1.exon3 | 2809925 | 2810064 |
| chr_1 | g9416 | g9416.t1 | cds | g9416.t1.CDS3 | 2809925 | 2810064 |
| chr_1 | g9416 | g9416.t1 | exon | g9416.t1.exon4 | 2810126 | 2811206 |
| chr_1 | g9416 | g9416.t1 | cds | g9416.t1.CDS4 | 2810126 | 2811206 |
| chr_1 | g9416 | g9416.t1 | TSS | g9416.t1 | NA | NA |
| chr_1 | g9416 | g9416.t1 | TTS | g9416.t1 | NA | NA |
Sequences
>g9416.t1 Gene=g9416 Length=2973
ATGAGACAAGAAATAGAAAACTTTTTCAATTCATCAACAATTCATGGCTTCAAATATTTT
TCAAAGAAATTTCATTGGATTGAAAGACTTTTTTGGATTTTGTCTTTGCTTTCTTCACTG
ATCATCACAATTTTTCTTATAACACAATTGATTATAAAAGTCAATAAAGTTCCAATTATT
ACTCATATCGCAGATCATCGTGTTTCAATTAAAGACATTGATTTTCCTGCAATCACTGTT
TGTGAAGGTTTTGTGCATGTTATTGACCATTATTTGGAAGAAGTCAAATATTATGAGAGA
CTTATGAATATTCCACGTTATTTTACAAATAGAACAAAAGAATTTTGCGATGAATTGAAA
TATTTTGATTATTTAAATTATGCTCAAATGTTAGATGAAATCGAAAATGGTGAATTTAAA
AACATTGATAAATTAGGAATGAAAATTCTCAAACGTCTTCAAGTTGCTGACATCATGACA
AATCGTGGAATTTTAACAAAACTAAACATTTCAGCTTCGACTGATGACTTTCTCGATGTT
CTAAATGAATTTCGATCTGATTTTGTAGTCGATTTTTTATGGAATCAATTAAAAGGCACA
AAATTTTCGTCAATTTTAACAGAATTTGGTCTATGCAGAACTTTTAATCTTACTTTACCA
GAAGACTTGTTCCATAAAGATTTAATATCTGATGATTTTGACCATAAATATTATTCAACT
TTCTCCAGAGAGAAAACTTTTGATTATAAACAAATTATTCCAAGAAGAGCAAACAGTGTT
GATGACACAGTTTTCTTTGAAGCTGAATGTTTTGAAATTTTATTAACTGAAACTTTCAAG
TTTGAAGGACAAATTTTAATGATTCATAATCCTTATGAGCTGCCGAACATACAAACAAAA
TCAATCATCATTAATGTTCGAGATAATTCAGTTGTTAATGTTAGACTCATTCCAGAAATG
TTTGACATTGATGAGTCATTGTATGATGATGATCCAATGGATCGAAATTGTTATTTTGAT
GATGAATTTGAACTCAAATTTTTCAAAGTTTACACAAAATCCAATTGTGAATCAGAATGC
CTAACAAATTACATGATCAAAAAATGCGGTTGTGTTGAATTTTATATGATTCGAAATTGC
ACAACCAGAATTTGCTCAGCTAGTGATAAAAGTTGCTATGATGAAACAAAAAATTCATTT
GAAAATCAAAAACATCAATGCAATTGTTTGCAACCTTGTTCTTATGTAAAATTTGATATC
GAACAATCACATGGAAGTGACACATGGAAAGAAAAATTTGCTTTGAAATTTATTTCAGCA
CCAAAACATATTTATTTAAAATACAAAGAAGCTTCGTTCCCAAAATTTGTACGGAAACAA
AGTTTTGACTTTTTAACGTTTCTCTCATTTGTTGGTGGTCTTTTGGGTTTGTTTGCAGGA
TTTTCATTGTTGTCAGCAGTTGAATTGTTTTATTGGTTTTTTGTGAGAATTTTTATAAGA
AAATGCAATAGAAAAACAACTATTGTGCATCCAATCAATGAAGAAATCAAAAAAGAACCA
AATATTGTCAAAGATTTCTTTAAAAATTCATCAATTCATGGATTGAGTTATGCAATTGAC
ACAAATCTTATCGAAAAATTATTTTGGACATTTTTTGTGACATTTTCGATAACTTCTTGC
ATTTTATTGTCGTCAAAAATAAGAAAATACAACACAATTGTTTATGAAGATAGCACATTG
CTTAAAAATAAGTTGAATTTTCCAGCAGTAACTTTCATACCAAGCTATTATGTTGACAGA
TATTTCTATGAAAATATTGCAAATAATTATGACCAAACAGATCTCAAAAGAAAAATTCTT
AAAAATCCAAAAACTTTAGATGATTTTCGAAAGAATTTAACTAAATACAGTGGATATCAT
AATTTCATATTAATAACTAAATTCGCAAATCTTACTTTTGAAACAATGTCAAGATACTTT
GAGATTATGAAAGAAAATTCAATGATTGATTGGTTTCAACAACAGTCTGCAACTTGGAAT
AATCAATATCAAGTGAATTTTACAATGACTCGAAATTTTTATGGAATGAGTTACACTTTT
AATTTAATGAACACAGAAAATATTTTGAGATACAAAAGAGTCAATGAAGAATTTCATTTA
AAAAGAAATGTTTCAATGTTGAATCAAAAATCAATGCCGAAAATTCAAAAGTATAAATAT
CCTTTAAAAGTTGGTGTAAATGATCGATCAAAATTTCAAATTAAAGCCTCAGAAAAAGAA
TTTGAAATTTACGATTTTATAAGAGCTCGATTTCAAAAAATAATAATTCATTCACCTGAA
CTTTTACCAACTTTTATGGACCTTAAAATGATCACTGATGTTCCACAATCGATTAATATT
GAAATCATAGCAGAAAATATCAAAACTGAAGAAAGTTTGCGTTCACAAAAACCTGAAGAT
CGAGAATGTTATTTTGAAGATGAAAGACAATTGAAATATTTTAAAATTTACACTCAAAAA
TATTGCGAAATGGAATGCATTATGGATCATTTATTTAATGTTTGTAAATGTCAAATGATT
GACTCAATTTATAAAGAAAGAAAACCATTTTGTTTAGCAATAAATGAAAATTGTATAAAT
ACAGAAAGAGCTAAAATATCACAACAAGAAAATAATATCAAAACTAACTGCACTTGTTTG
CCACTATGCAATATGCTAACTTATCATGTCAAATATAATCAGATTAAAAATGAAGACAAT
GAGTCAATTATAACAGTAACAATGAATGGAGAAGATTTCATTCTATTTCGTCGTTATCAA
CAATTTACTTTTTCTGATATTGTATCATATGTTGGTGGATTGCTTGGGCTTTTTGCTGGA
ATTTCTTTGTTGTCAATTATTGAAATTTTCTATTTTATTACGATCAGATTAATTAATGAC
TTTTTAAAATGTTTAAGAATGAAAAGAAGTTAA
>g9416.t1 Gene=g9416 Length=990
MRQEIENFFNSSTIHGFKYFSKKFHWIERLFWILSLLSSLIITIFLITQLIIKVNKVPII
THIADHRVSIKDIDFPAITVCEGFVHVIDHYLEEVKYYERLMNIPRYFTNRTKEFCDELK
YFDYLNYAQMLDEIENGEFKNIDKLGMKILKRLQVADIMTNRGILTKLNISASTDDFLDV
LNEFRSDFVVDFLWNQLKGTKFSSILTEFGLCRTFNLTLPEDLFHKDLISDDFDHKYYST
FSREKTFDYKQIIPRRANSVDDTVFFEAECFEILLTETFKFEGQILMIHNPYELPNIQTK
SIIINVRDNSVVNVRLIPEMFDIDESLYDDDPMDRNCYFDDEFELKFFKVYTKSNCESEC
LTNYMIKKCGCVEFYMIRNCTTRICSASDKSCYDETKNSFENQKHQCNCLQPCSYVKFDI
EQSHGSDTWKEKFALKFISAPKHIYLKYKEASFPKFVRKQSFDFLTFLSFVGGLLGLFAG
FSLLSAVELFYWFFVRIFIRKCNRKTTIVHPINEEIKKEPNIVKDFFKNSSIHGLSYAID
TNLIEKLFWTFFVTFSITSCILLSSKIRKYNTIVYEDSTLLKNKLNFPAVTFIPSYYVDR
YFYENIANNYDQTDLKRKILKNPKTLDDFRKNLTKYSGYHNFILITKFANLTFETMSRYF
EIMKENSMIDWFQQQSATWNNQYQVNFTMTRNFYGMSYTFNLMNTENILRYKRVNEEFHL
KRNVSMLNQKSMPKIQKYKYPLKVGVNDRSKFQIKASEKEFEIYDFIRARFQKIIIHSPE
LLPTFMDLKMITDVPQSINIEIIAENIKTEESLRSQKPEDRECYFEDERQLKYFKIYTQK
YCEMECIMDHLFNVCKCQMIDSIYKERKPFCLAINENCINTERAKISQQENNIKTNCTCL
PLCNMLTYHVKYNQIKNEDNESIITVTMNGEDFILFRRYQQFTFSDIVSYVGGLLGLFAG
ISLLSIIEIFYFITIRLINDFLKCLRMKRS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 18 | g9416.t1 | Gene3D | G3DSA:1.10.287.770 | - | 29 | 490 | 5.6E-39 |
| 13 | g9416.t1 | Gene3D | G3DSA:2.60.470.10 | - | 59 | 460 | 5.6E-39 |
| 14 | g9416.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 85 | 283 | 5.6E-39 |
| 17 | g9416.t1 | Gene3D | G3DSA:1.10.287.820 | - | 329 | 413 | 5.6E-39 |
| 19 | g9416.t1 | Gene3D | G3DSA:1.10.287.770 | - | 546 | 970 | 4.3E-22 |
| 12 | g9416.t1 | Gene3D | G3DSA:2.60.470.10 | - | 572 | 940 | 4.3E-22 |
| 15 | g9416.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 596 | 770 | 4.3E-22 |
| 16 | g9416.t1 | Gene3D | G3DSA:1.10.287.820 | - | 815 | 903 | 4.3E-22 |
| 4 | g9416.t1 | PANTHER | PTHR11690:SF222 | ACID-SENSING ION CHANNEL 5 | 4 | 503 | 3.0E-90 |
| 6 | g9416.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 4 | 503 | 3.0E-90 |
| 3 | g9416.t1 | PANTHER | PTHR11690:SF222 | ACID-SENSING ION CHANNEL 5 | 522 | 977 | 3.0E-90 |
| 5 | g9416.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 522 | 977 | 3.0E-90 |
| 2 | g9416.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 8 | 491 | 9.5E-69 |
| 1 | g9416.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 526 | 972 | 2.9E-46 |
| 21 | g9416.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 29 | - |
| 28 | g9416.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 52 | - |
| 24 | g9416.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 53 | 463 | - |
| 26 | g9416.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 464 | 495 | - |
| 20 | g9416.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 496 | 546 | - |
| 25 | g9416.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 547 | 565 | - |
| 23 | g9416.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 566 | 946 | - |
| 27 | g9416.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 947 | 973 | - |
| 22 | g9416.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 974 | 990 | - |
| 11 | g9416.t1 | ProSitePatterns | PS01206 | Amiloride-sensitive sodium channels signature. | 351 | 371 | - |
| 10 | g9416.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 52 | - |
| 9 | g9416.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 473 | 495 | - |
| 8 | g9416.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 547 | 565 | - |
| 7 | g9416.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 951 | 973 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed