General information for g9421.t12
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as GTP-binding protein Rheb-like protein.
Parent gene
- The parent gene g9421 is located on chr_1: 2843368..2844292 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9421 | g9421.t12 | isoform | g9421.t12 | 2842519 | 2844292 |
| chr_1 | g9421 | g9421.t12 | exon | g9421.t12.exon1 | 2842519 | 2842869 |
| chr_1 | g9421 | g9421.t12 | TTS | g9421.t12 | 2842526 | 2842526 |
| chr_1 | g9421 | g9421.t12 | exon | g9421.t12.exon2 | 2842909 | 2843257 |
| chr_1 | g9421 | g9421.t12 | cds | g9421.t12.CDS1 | 2843032 | 2843257 |
| chr_1 | g9421 | g9421.t12 | exon | g9421.t12.exon3 | 2843318 | 2843535 |
| chr_1 | g9421 | g9421.t12 | cds | g9421.t12.CDS2 | 2843318 | 2843535 |
| chr_1 | g9421 | g9421.t12 | exon | g9421.t12.exon4 | 2843595 | 2843623 |
| chr_1 | g9421 | g9421.t12 | cds | g9421.t12.CDS3 | 2843595 | 2843623 |
| chr_1 | g9421 | g9421.t12 | exon | g9421.t12.exon5 | 2844089 | 2844121 |
| chr_1 | g9421 | g9421.t12 | cds | g9421.t12.CDS4 | 2844089 | 2844121 |
| chr_1 | g9421 | g9421.t12 | exon | g9421.t12.exon6 | 2844244 | 2844292 |
| chr_1 | g9421 | g9421.t12 | cds | g9421.t12.CDS5 | 2844244 | 2844292 |
| chr_1 | g9421 | g9421.t12 | TSS | g9421.t12 | 2844383 | 2844383 |
Sequences
>g9421.t12 Gene=g9421 Length=1029
ATGAAAGGAAGAACAAGAAATTTGGCCATTATGGGATATAGAAGTGTGGGTAAATCATCG
CTTTCAATACAGTTTACCGAAGGTCAGTTTGTTGATAGTTATGATCCAACTATTGAAAGG
ACTTTTACAAAACATACGAGAATAAATAATGTGGACTATGAAATAAAGCTTGTTGATACA
GCTGGTCAAGATGAGTACTCGATTTTTCCTGCTCAATATTCTATGGATTATCATGGATAT
GTCTTAGTTTATTCAATCACAAGTCAAAAATCATTCGAAGTTATCAAAATTATTTATGAT
AAACTAGTAGATGTGATGGGAAAACCTTACGTTCCTGTTGTCTTAGTTGGAAATAAAACT
GATTTACATCAAGAACGAGCGGTGTCATTTGAGGAAGGAAAACGTACTGCTGAATCATGG
AAAGCTGTATTTTTGGAAACGTCTGCCAAACAAAATGAATCTGTCGATGAAATCTTCCTA
CAGCTTTTACAATTAGTTGAAAAAGAAAGTAATGGAAGTAATCCACCTGCAAAGGGAAAT
TGCACTATATCTTAATTATTTTGGCATTCACAAGAACACACACACACACCATTAATGATC
ACCACTCTACATTTCAATCAACTGACACATTTTAAAAGCTGGCAATAATGAAATTTAGTA
ATTTAAGTTTTTATCAAACTACTTTATGTGTGATAATATGTCAATGAACTATTATTGCTA
TTAGCTAAATATAATAATTAAATTTAGCTTAAACAAAAAAAATTAATTTTGAGTTCATAT
GCATGAAGTAGCCATTTCTGTGTACAACTATAATTTTTCATACAACTGTTACTCACACAA
AAAAAAACGCTACCTACCATAATAATTGTCTCTTATTTTTTCAACTCTAATTTTTTAACG
AAACTAGCTTTATGTAATATGGAAAATATTCTTTCCAAAAATTTATTGCTAAATTGGCAT
TCAACATTTTTTTATTTTTGAATTTTTAGGGCAATAAAAGTGGATTACTTGAGAAAAAAA
GTATTTAAA
>g9421.t12 Gene=g9421 Length=184
MKGRTRNLAIMGYRSVGKSSLSIQFTEGQFVDSYDPTIERTFTKHTRINNVDYEIKLVDT
AGQDEYSIFPAQYSMDYHGYVLVYSITSQKSFEVIKIIYDKLVDVMGKPYVPVVLVGNKT
DLHQERAVSFEEGKRTAESWKAVFLETSAKQNESVDEIFLQLLQLVEKESNGSNPPAKGN
CTIS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g9421.t12 | CDD | cd04137 | RheB | 6 | 184 | 0.000 |
| 10 | g9421.t12 | Gene3D | G3DSA:3.40.50.300 | - | 2 | 175 | 0.000 |
| 2 | g9421.t12 | PANTHER | PTHR24070 | RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY | 4 | 183 | 0.000 |
| 3 | g9421.t12 | PANTHER | PTHR24070:SF428 | RAS HOMOLOG, MTORC1-BINDING-LIKE 1 | 4 | 183 | 0.000 |
| 5 | g9421.t12 | PRINTS | PR00449 | Transforming protein P21 ras signature | 6 | 27 | 0.000 |
| 4 | g9421.t12 | PRINTS | PR00449 | Transforming protein P21 ras signature | 29 | 45 | 0.000 |
| 7 | g9421.t12 | PRINTS | PR00449 | Transforming protein P21 ras signature | 46 | 68 | 0.000 |
| 6 | g9421.t12 | PRINTS | PR00449 | Transforming protein P21 ras signature | 109 | 122 | 0.000 |
| 8 | g9421.t12 | PRINTS | PR00449 | Transforming protein P21 ras signature | 144 | 166 | 0.000 |
| 1 | g9421.t12 | Pfam | PF00071 | Ras family | 8 | 166 | 0.000 |
| 16 | g9421.t12 | ProSiteProfiles | PS51421 | small GTPase Ras family profile. | 1 | 184 | 24.581 |
| 12 | g9421.t12 | SMART | SM00173 | ras_sub_4 | 3 | 169 | 0.000 |
| 14 | g9421.t12 | SMART | SM00174 | rho_sub_3 | 8 | 169 | 0.000 |
| 13 | g9421.t12 | SMART | SM00175 | rab_sub_5 | 10 | 169 | 0.000 |
| 9 | g9421.t12 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 5 | 166 | 0.000 |
| 15 | g9421.t12 | TIGRFAM | TIGR00231 | small_GTP: small GTP-binding protein domain | 4 | 159 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0007165 | signal transduction | BP |
| GO:0005525 | GTP binding | MF |
| GO:0003924 | GTPase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K07208 (Ras homolog enriched in brain)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed