General information for g9445.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Fatty acid synthase.
Parent gene
- The parent gene g9445 is located on chr_1: 3081066..3083491 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9445 | g9445.t1 | isoform | g9445.t1 | 3081066 | 3083491 |
| chr_1 | g9445 | g9445.t1 | exon | g9445.t1.exon1 | 3081066 | 3081148 |
| chr_1 | g9445 | g9445.t1 | cds | g9445.t1.CDS1 | 3081066 | 3081148 |
| chr_1 | g9445 | g9445.t1 | exon | g9445.t1.exon2 | 3081569 | 3081706 |
| chr_1 | g9445 | g9445.t1 | cds | g9445.t1.CDS2 | 3081569 | 3081706 |
| chr_1 | g9445 | g9445.t1 | exon | g9445.t1.exon3 | 3081765 | 3081781 |
| chr_1 | g9445 | g9445.t1 | cds | g9445.t1.CDS3 | 3081765 | 3081781 |
| chr_1 | g9445 | g9445.t1 | exon | g9445.t1.exon4 | 3081836 | 3082827 |
| chr_1 | g9445 | g9445.t1 | cds | g9445.t1.CDS4 | 3081836 | 3082827 |
| chr_1 | g9445 | g9445.t1 | exon | g9445.t1.exon5 | 3082886 | 3083491 |
| chr_1 | g9445 | g9445.t1 | cds | g9445.t1.CDS5 | 3082886 | 3083491 |
| chr_1 | g9445 | g9445.t1 | TTS | g9445.t1 | 3084001 | 3084001 |
| chr_1 | g9445 | g9445.t1 | TSS | g9445.t1 | NA | NA |
Sequences
>g9445.t1 Gene=g9445 Length=1836
ATGATTATGACACAAACTGATGGCAGAGGTGTCGATTATGTTCTTAATTCATTGTCAGAA
GAAAAACTTAAAGCATCAATCAAAACATTCTTAATATATTCTGGCGGTTTGGGTGGTTTT
GGTCTCGAGCTTGCTGATTGGTTAGTTTTGAGAGGATGCAGAAAACTTGTCTTGAGTTCA
GGTCGTGGAATAACAAAACAATATCAAGCTTATCGCATCAAAATTTGGGAATCATATGCA
ATTGATCAAAGAAGCGATGAAACTTGGACCAGTTGGTGGCATCGTGATGCTATTTTTGAA
AATCAAGACTCAAAGTCGTTTGTTGAGAGTATGGGACCAAAAGCAATTGCAACAAAATAT
CTTGATGAACTTTCAAGAACTTTGTGTCCCGAATTGCAATATTTTGTTGTCTTCTCAAGT
GTTTCATGCGGACGTGGAAATGCTGGTCAAACGAATTATGGAATGGCTAACTCTGTGATG
GAAAGAATTATGGAGCAAAGAAATAAATTTGGATTGCCAGCAAAAGCGATTCAATGGGGT
GCAGTTGGTGAGGTCGGTTTGGTTGCTGATATGCAAGAAAATCATACAGTAGATATGGTG
ATTGGTGGAACATTGCAGCAAAGAATTTCATCTTGCTTGGAAGAACTCGATCCGCTCATT
CTTTCGCCTCATCCAATTGTCTCAAGCATGGTTGTGGCTGAAAAGCGCTTTACTTTGGAT
TCATCAGCTAATATAATTGACACAATTATGAATGTCATGTCGATTAAAGACAAAAAATCA
ATTTCAATGGAAACAACTCTTTCTGAATTGGGTATGGACTCATTGATGACAGTTGAAATT
CAACAATTGCTTGAACGTGAATATGACGTTGTCATTTCGCCTCAAGAACTACGTTCAATG
AGTTTAGCACAACTTCAAAAATGTGTGCATAACCGTGATGGAACAGAAACAGGACAAAAA
GTAAAAATTGTCAATGAAAATGTTCCTAAAGGTGTGCAATTGTTATTGAGAAATTTGGGA
GATGAAACAAACAGTACAAAGAGCATTTTGAAACTTCAGAGCATGTCAGATGAAGGTATA
AAAGCATTGATAATTCCGGGTCTTGAAGGAATGGCGGGAAATGCATGGTACGATATTGCT
AAAAATTTGAGGTATCCAACTTACATCTTGCAAACTTTGAATACATGGAAGTCAAGAAAT
TTGGATGAAATTTATGATGGAGTTATTGAAGAAATTTTGAATTTATTTAAAAATGAAAAT
GAATTTGTTTTAATTGGTTATTCATTCGGTGCAATGTTAACATTGAAAGTAGCAAAAGCA
CTAGAGAACTTAGGAAAGATTGGACGAATAGCTCTTATTGATGGTGCACCGAAATTTCTT
AAAAAGCTTGCACTTGATCACCTTCCACAAAATTATACTGAAGAAACAGTTCAGAATATT
ATTTTGATCAACACTATCTATACTTGCTTTCCTGAAGACAATGGTGTGATTGCTAGAGAA
GTTCTTGCTTTATCAAGTTGGGATGCACGAGTTCGAAAATTGACAGACTTGTACAAAGAC
AAGAAACTTTATAGTGAGGAATATGGCCTTAAAATGTGCAATGCACTTGTCAATCGTATG
ATGTTGACAGCTGTTATCGATACAAGTACATTTGGCGTCATTAATAATGCACCTTTAACC
CTGATTCGTCCTTCAGATCATTCTGTACAGGAAATTGAAGAAAATTATTGTCTTGCTCAA
TATACACCAAATACAATCAACATCAAATACATGGAAGGCAATCATGCAACAATTTTAGAA
AATTCCAAATTACCAATTGCACTCAACTCTATTTAA
>g9445.t1 Gene=g9445 Length=611
MIMTQTDGRGVDYVLNSLSEEKLKASIKTFLIYSGGLGGFGLELADWLVLRGCRKLVLSS
GRGITKQYQAYRIKIWESYAIDQRSDETWTSWWHRDAIFENQDSKSFVESMGPKAIATKY
LDELSRTLCPELQYFVVFSSVSCGRGNAGQTNYGMANSVMERIMEQRNKFGLPAKAIQWG
AVGEVGLVADMQENHTVDMVIGGTLQQRISSCLEELDPLILSPHPIVSSMVVAEKRFTLD
SSANIIDTIMNVMSIKDKKSISMETTLSELGMDSLMTVEIQQLLEREYDVVISPQELRSM
SLAQLQKCVHNRDGTETGQKVKIVNENVPKGVQLLLRNLGDETNSTKSILKLQSMSDEGI
KALIIPGLEGMAGNAWYDIAKNLRYPTYILQTLNTWKSRNLDEIYDGVIEEILNLFKNEN
EFVLIGYSFGAMLTLKVAKALENLGKIGRIALIDGAPKFLKKLALDHLPQNYTEETVQNI
ILINTIYTCFPEDNGVIAREVLALSSWDARVRKLTDLYKDKKLYSEEYGLKMCNALVNRM
MLTAVIDTSTFGVINNAPLTLIRPSDHSVQEIEENYCLAQYTPNTINIKYMEGNHATILE
NSKLPIALNSI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g9445.t1 | Gene3D | G3DSA:3.40.50.720 | - | 93 | 240 | 0.00000 |
| 17 | g9445.t1 | Gene3D | G3DSA:1.10.1200.10 | - | 241 | 322 | 0.00000 |
| 19 | g9445.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 363 | 604 | 0.00000 |
| 18 | g9445.t1 | Gene3D | G3DSA:1.10.1470.20 | Fatty acid synthase; domain 2 | 460 | 546 | 0.00000 |
| 7 | g9445.t1 | PANTHER | PTHR43775 | FATTY ACID SYNTHASE | 1 | 28 | 0.00000 |
| 10 | g9445.t1 | PANTHER | PTHR43775:SF23 | FATTY ACID SYNTHASE 3 | 1 | 28 | 0.00000 |
| 5 | g9445.t1 | PANTHER | PTHR43775 | FATTY ACID SYNTHASE | 28 | 82 | 0.00000 |
| 8 | g9445.t1 | PANTHER | PTHR43775:SF23 | FATTY ACID SYNTHASE 3 | 28 | 82 | 0.00000 |
| 6 | g9445.t1 | PANTHER | PTHR43775 | FATTY ACID SYNTHASE | 95 | 606 | 0.00000 |
| 9 | g9445.t1 | PANTHER | PTHR43775:SF23 | FATTY ACID SYNTHASE 3 | 95 | 606 | 0.00000 |
| 3 | g9445.t1 | Pfam | PF08659 | KR domain | 34 | 77 | 0.00008 |
| 4 | g9445.t1 | Pfam | PF08659 | KR domain | 95 | 183 | 0.00000 |
| 2 | g9445.t1 | Pfam | PF00550 | Phosphopantetheine attachment site | 257 | 305 | 0.00000 |
| 1 | g9445.t1 | Pfam | PF00975 | Thioesterase domain | 366 | 604 | 0.00000 |
| 20 | g9445.t1 | ProSiteProfiles | PS50075 | Carrier protein (CP) domain profile. | 236 | 316 | 10.50900 |
| 15 | g9445.t1 | SMART | SM00822 | This enzymatic domain is part of bacterial polyketide synthases and catalyses the first step in the reductive modification of the beta-carbonyl centres in the growing polyketide chain. It uses NADPH to reduce the keto group to a hydroxy group. | 28 | 185 | 0.00000 |
| 14 | g9445.t1 | SMART | SM00823 | Phosphopantetheine attachment site | 242 | 297 | 0.00150 |
| 12 | g9445.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 35 | 198 | 0.00000 |
| 13 | g9445.t1 | SUPERFAMILY | SSF47336 | ACP-like | 245 | 311 | 0.00000 |
| 11 | g9445.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 336 | 602 | 0.00000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0031177 | phosphopantetheine binding | MF |
| GO:0009058 | biosynthetic process | BP |
| GO:0004312 | fatty acid synthase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed