General information for g9632.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Mitotic checkpoint serine/threonine-protein kinase BUB1.
Parent gene
- The parent gene g9632 is located on chr_1: 4374072..4375489 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9632 | g9632.t1 | TSS | g9632.t1 | 4369505 | 4369505 |
| chr_1 | g9632 | g9632.t1 | isoform | g9632.t1 | 4369998 | 4374072 |
| chr_1 | g9632 | g9632.t1 | exon | g9632.t1.exon1 | 4369998 | 4370044 |
| chr_1 | g9632 | g9632.t1 | cds | g9632.t1.CDS1 | 4369998 | 4370044 |
| chr_1 | g9632 | g9632.t1 | exon | g9632.t1.exon2 | 4370121 | 4370319 |
| chr_1 | g9632 | g9632.t1 | cds | g9632.t1.CDS2 | 4370121 | 4370319 |
| chr_1 | g9632 | g9632.t1 | exon | g9632.t1.exon3 | 4370393 | 4372965 |
| chr_1 | g9632 | g9632.t1 | cds | g9632.t1.CDS3 | 4370393 | 4372965 |
| chr_1 | g9632 | g9632.t1 | exon | g9632.t1.exon4 | 4373018 | 4373138 |
| chr_1 | g9632 | g9632.t1 | cds | g9632.t1.CDS4 | 4373018 | 4373138 |
| chr_1 | g9632 | g9632.t1 | exon | g9632.t1.exon5 | 4373204 | 4373451 |
| chr_1 | g9632 | g9632.t1 | cds | g9632.t1.CDS5 | 4373204 | 4373451 |
| chr_1 | g9632 | g9632.t1 | exon | g9632.t1.exon6 | 4373512 | 4373608 |
| chr_1 | g9632 | g9632.t1 | cds | g9632.t1.CDS6 | 4373512 | 4373608 |
| chr_1 | g9632 | g9632.t1 | exon | g9632.t1.exon7 | 4373668 | 4373837 |
| chr_1 | g9632 | g9632.t1 | cds | g9632.t1.CDS7 | 4373668 | 4373837 |
| chr_1 | g9632 | g9632.t1 | exon | g9632.t1.exon8 | 4373901 | 4374072 |
| chr_1 | g9632 | g9632.t1 | cds | g9632.t1.CDS8 | 4373901 | 4374072 |
| chr_1 | g9632 | g9632.t1 | TTS | g9632.t1 | 4374662 | 4374662 |
Sequences
>g9632.t1 Gene=g9632 Length=3627
ATGAATCCACTTAATAATTCAATTAATTCACTTCAAAATGAAATTGGACAATGGGAACAT
GCTCTAAATAATAACAATAACGGAAATGACCCACTTGATCTTTGGTGGAAATATATTTGC
TGGCTTGAGAATCATCAAGAACTTGAAGCCAAATTTCGAAAAACGGTAGAGCAATGTCTT
TCAATTTATGACAAGTATGAAATTTACAAACAAGATTTACGTCTTGTTAAACTTTACATT
AAATATATTGATTTTCAACCAAATCCTTTGAATTTATATAAAATTTTATATCAGCAAGGC
ATAGGAACAAAATGTGCAGCATTTTACATTGGATGGGCACATTATTATAACTCTGCAAAT
CATTTTAAACAAGCAGAATCTATATACAATTTGGGTGTACAATTGAAGGCTGAACCACTT
AATGAATTGGAAACTGCTCAAAAGAATTTTCGCTTTTCTGTCGCACAACGGATGCTTTAT
AATGATGAATCTTCAAAGAAGAGAACAATTTCGTCTCTTGAGGAGCAAAGACAACAAATA
ACATCGCTCTCTCCTCATCAAATACACTCAGCTAAAAGAATGAGAAGTGATTCTCAAAAT
GATCAGTATAATACAACAACAAATTCTCAACAATATGCAAATAATCATGAATATAATAAT
TACAACAACTCGAATTATAATTCCAATGTTTCTCCAAATGATGCGAGCTATGCAATTTGT
TCATCATTAAGCTATGTTTATGATGACTCAAATGGCTATGCTTCTATGGAGGAGCAACCA
CAACAGCATCAAAAGCCAATTACTTACACTTTCAATTGCGGTTTTCAAATGCCACCGAAT
TTTATTAACTATGCTCGTAATTCAGATGATCCATGGAATGTTCCATTGTGTCTTGAAGAG
CCTTATGATCCAAATCGTCGATGCTATTATCCAAAAAAATCGGTATATTCTGGTGATGAT
TGTGAATACAGTATTGAAGAATTAAAAGCTCATAAATGGCGCTTAAAGATAGAAGAAAAG
AGGCGTGAAGAGGAGATGAGACGACAGCGAGAGTATGAAGAAAAACTTCAAAAAGAGCAA
CAAGAGAAAGAGAGATTGCGACAAGAGACTGAAGCTAAAAGACAAAGAGAAGAGCAAGAA
CGTATTCGAAAAGAACAGGAAGCTCAAAGAATTAAAATGGAACAAGATAAACTTCAACGA
CAACGTGAAGATACTGAAAGATTAAGAAGACAACAAGAGGAAGAACAAATGAATCGCTAT
AATCAATCTCAAATGTATCAAATGCAACAACAACAGCAACAAAGATGGAATTACCATCAA
CAACAACAACACAGTCCAGCTAATTATCCACCAAATTATCATCATGGCTCGTATTATCAT
CAAAGTCCACAATCTTATTCTTATTCACATGATCATCAATATCAAACTCAAACAAATATG
TATTCACAATCAAATATGTATCATAGTCCACATTATCAAAATTCACCAAATTATGCACCA
CAACAACAAAGTGTTATTGTTAATCGTACTCATTACGAAAATCATCAATATTATATGAAT
TATCCAACACATTCTCCTGTACATCAATCACAAGTTCCATTATCGCCCTATCATCACCAT
CAGCAGCAGCCACCTTCACATATTCATCATTCTCCTCATCAAATGCCACATCAGCAACAA
ATGATTTCACATCCAAATCAAATTTCAAATGAGCCAAATAAAACATTGATGGGAACACAG
CAGCATCAACAGAATTTATATAATAATCAAACACAATCCGTTAATCATACAAAATCTCAT
TATAATGAACCTGATTATCAAGATGTTGAATATTTGATTGATCATCAAGCTGAAATTGAC
CCAAAAATGTTACAACAACCAATAATTGAAGGAAATAGCAAAGAATATACAAATGGAGCT
TCTGATGAAAGTGAATCGTATGAAGATGATGATGATGATGAAGAAGAAGAGGAAGACGAT
GAGAATGAAGAGGAACAAATTCCAACAATTGTCAATTCATATATGCTCGATGATTTAGAA
GAACAGATTGAAGCCAGTACTATAAGTTTTTCATCAAACGGAAAATCACGTGATAAAAAA
ATAACGATCAAATTTCGCAAAGAGAAAACAACAACGACAATCATTAATTCTGAATCAAAT
TCCGAATCTTCTCAAAGTCAAACTCAGCAGAAGATACCTTTAGAATCTACATCAACAACG
TCATCAATTAATGAAAAGAAGAAAAAGAAAAAAAATAAAGATATACTTTCTTCATATGAT
GGCGAAAATACACAATTTATGCCAAGTGCAACAAATAGCTGCTCATCGACAAATAATGGC
GAAACAAATAATAATCAAAACATTTCCTTTAGTAAAATAACATTTAATGGATGTGTGACG
CCAATAAGAAAACCAGGTGCAAGTAAAACTTCAACACCTATTAGCACCTATAAATATCTT
AAAAATAAAAGCTCATTCATTTCTAATCAAAATGACGATAGCTTATGCTCTTATAATGGT
GATCAAAATTCATTTTTCCAAGCTGAAAATGATGATGAATATAAATCTCGCAGATTAGAA
AAAGCTTTAGCAACAATTGATGAGCATATGAAGAAACGTGACATTGATCCATTCAATTCA
GAGCTCTGTCGTGCATTCTTAACAAAACTTAATTTTCCAAATCCAGAAAATTCCAACGAT
TATACAGTTACAAATGTCAACTTACCAAAATTGATTAAAAATCAAACAGTTCCTATAAGT
GGAATAAGCTATCAAATTGAAAAAGAAGTAGGCCGTGGTGCATATGGTGCAGTTTTTCGG
GGAATAAATTTGATGGATGGCAGTGCTGTTGCATTAAAATATCAACGACCATCAAATATG
GCCACATGGGAGCTTTATATTTGTACAGAATTAAAGAATAGAATTAAGAATGCTGATATT
CTTCCTGGGTTTATGACTATATCCGCTGCAGTCATTGCACCAAATGCAAGTGTATTAATT
TCGGAATTTTCTCAATATGGATCTTTATTAGACATTAATAACCGCATAAGAACGGCTACA
ACTAAAGTTATGCATGAAAGTTTAGTAATGCATTTTTCGAGTCAAATACTTAATATTGTT
GCACATTTGCACGACTGCAAAATCATTCATGCAGATATCAAACCCGATAACTGGCTATTA
ATGACATTACCCTCAGCAGATACTCATATTCCAAGCTTAAGGTTAATTGATTTTGGTTGT
GCAATCGACATGACACTCTTTAAAGAAGGACAGGAATTTAGAAAGATAATTGAAACTGAT
GGTTTTACATGTGTTGAAATGCAAGAGGGTAGATTATGGTCATATCAGACTGACTTATTT
TGTGTCGCTGGTACAATACATGTAATGTTGTTTGGTGAATATATGCAAGTGAATAAGAAA
TTCGGTCAATGGGAAATTAAACAAAAATTTCCTAGGTATCTTAATAAAACTTTATGGACT
GATATATTCTCGAAATTGCTGAACATAAAAGATAGTAATAACTTGCCAAAATTACGTCAA
ATGAAAGCTCAAATTGATCGAGAAATAAATGACAACGAACTTGTTAAACATATTCGAACA
GTTAGCAATCTTATAAGAAAACGCTAA
>g9632.t1 Gene=g9632 Length=1208
MNPLNNSINSLQNEIGQWEHALNNNNNGNDPLDLWWKYICWLENHQELEAKFRKTVEQCL
SIYDKYEIYKQDLRLVKLYIKYIDFQPNPLNLYKILYQQGIGTKCAAFYIGWAHYYNSAN
HFKQAESIYNLGVQLKAEPLNELETAQKNFRFSVAQRMLYNDESSKKRTISSLEEQRQQI
TSLSPHQIHSAKRMRSDSQNDQYNTTTNSQQYANNHEYNNYNNSNYNSNVSPNDASYAIC
SSLSYVYDDSNGYASMEEQPQQHQKPITYTFNCGFQMPPNFINYARNSDDPWNVPLCLEE
PYDPNRRCYYPKKSVYSGDDCEYSIEELKAHKWRLKIEEKRREEEMRRQREYEEKLQKEQ
QEKERLRQETEAKRQREEQERIRKEQEAQRIKMEQDKLQRQREDTERLRRQQEEEQMNRY
NQSQMYQMQQQQQQRWNYHQQQQHSPANYPPNYHHGSYYHQSPQSYSYSHDHQYQTQTNM
YSQSNMYHSPHYQNSPNYAPQQQSVIVNRTHYENHQYYMNYPTHSPVHQSQVPLSPYHHH
QQQPPSHIHHSPHQMPHQQQMISHPNQISNEPNKTLMGTQQHQQNLYNNQTQSVNHTKSH
YNEPDYQDVEYLIDHQAEIDPKMLQQPIIEGNSKEYTNGASDESESYEDDDDDEEEEEDD
ENEEEQIPTIVNSYMLDDLEEQIEASTISFSSNGKSRDKKITIKFRKEKTTTTIINSESN
SESSQSQTQQKIPLESTSTTSSINEKKKKKKNKDILSSYDGENTQFMPSATNSCSSTNNG
ETNNNQNISFSKITFNGCVTPIRKPGASKTSTPISTYKYLKNKSSFISNQNDDSLCSYNG
DQNSFFQAENDDEYKSRRLEKALATIDEHMKKRDIDPFNSELCRAFLTKLNFPNPENSND
YTVTNVNLPKLIKNQTVPISGISYQIEKEVGRGAYGAVFRGINLMDGSAVALKYQRPSNM
ATWELYICTELKNRIKNADILPGFMTISAAVIAPNASVLISEFSQYGSLLDINNRIRTAT
TKVMHESLVMHFSSQILNIVAHLHDCKIIHADIKPDNWLLMTLPSADTHIPSLRLIDFGC
AIDMTLFKEGQEFRKIIETDGFTCVEMQEGRLWSYQTDLFCVAGTIHVMLFGEYMQVNKK
FGQWEIKQKFPRYLNKTLWTDIFSKLLNIKDSNNLPKLRQMKAQIDREINDNELVKHIRT
VSNLIRKR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g9632.t1 | CDD | cd13981 | STKc_Bub1_BubR1 | 923 | 1207 | 4.31716E-102 |
| 8 | g9632.t1 | Coils | Coil | Coil | 335 | 418 | - |
| 6 | g9632.t1 | Gene3D | G3DSA:1.25.40.430 | - | 14 | 172 | 2.2E-41 |
| 7 | g9632.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 922 | 1208 | 2.2E-90 |
| 17 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 169 | 205 | - |
| 13 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 174 | 189 | - |
| 16 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 352 | 416 | - |
| 22 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 352 | 445 | - |
| 20 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 417 | 445 | - |
| 15 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 529 | 562 | - |
| 14 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 632 | 668 | - |
| 18 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 642 | 666 | - |
| 19 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 712 | 741 | - |
| 21 | g9632.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 712 | 754 | - |
| 3 | g9632.t1 | PANTHER | PTHR14030:SF4 | BUB1 KINASE, ISOFORM A-RELATED | 10 | 1193 | 1.3E-118 |
| 4 | g9632.t1 | PANTHER | PTHR14030 | MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 | 10 | 1193 | 1.3E-118 |
| 1 | g9632.t1 | Pfam | PF08311 | Mad3/BUB1 homology region 1 | 17 | 133 | 4.6E-33 |
| 2 | g9632.t1 | Pfam | PF00069 | Protein kinase domain | 924 | 1137 | 1.7E-22 |
| 12 | g9632.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 930 | 953 | - |
| 23 | g9632.t1 | ProSiteProfiles | PS51489 | BUB1 N-terminal domain profile. | 18 | 182 | 39.465 |
| 24 | g9632.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 924 | 1208 | 20.188 |
| 10 | g9632.t1 | SMART | SM00777 | mad3_bub1_i | 11 | 133 | 1.5E-38 |
| 11 | g9632.t1 | SMART | SM00220 | serkin_6 | 924 | 1189 | 1.1E-9 |
| 5 | g9632.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 924 | 1167 | 2.77E-37 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0007094 | mitotic spindle assembly checkpoint signaling | BP |
KEGG
Orthology
- This transcript has been annotated with K02178 (checkpoint serine/threonine-protein kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
