General information for g9632.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Mitotic checkpoint serine/threonine-protein kinase BUB1 beta.
Parent gene
- The parent gene g9632 is located on chr_1: 4374072..4375489 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9632 | g9632.t2 | isoform | g9632.t2 | 4369505 | 4370738 |
| chr_1 | g9632 | g9632.t2 | TSS | g9632.t2 | 4369505 | 4369505 |
| chr_1 | g9632 | g9632.t2 | exon | g9632.t2.exon1 | 4369505 | 4369617 |
| chr_1 | g9632 | g9632.t2 | exon | g9632.t2.exon2 | 4369925 | 4370044 |
| chr_1 | g9632 | g9632.t2 | cds | g9632.t2.CDS1 | 4369998 | 4370044 |
| chr_1 | g9632 | g9632.t2 | exon | g9632.t2.exon3 | 4370121 | 4370319 |
| chr_1 | g9632 | g9632.t2 | cds | g9632.t2.CDS2 | 4370121 | 4370319 |
| chr_1 | g9632 | g9632.t2 | exon | g9632.t2.exon4 | 4370393 | 4370738 |
| chr_1 | g9632 | g9632.t2 | cds | g9632.t2.CDS3 | 4370393 | 4370737 |
| chr_1 | g9632 | g9632.t2 | TTS | g9632.t2 | NA | NA |
Sequences
>g9632.t2 Gene=g9632 Length=778
TGAGCGTTTAAGAAAAAGTGAAAGTTGAAAGCCCCCGAAAAAAATACATTTCTTAATTTA
TCGTAATTTTACACCTATATTAGATTTCTAACTTCAATATTCCCACTAAATAGTGATTGC
AGCGACTTGCGATCTATAAAAGTTCTTAATGCAGATATTTTTGTGATTTAAAAAATCAAC
CAAGACATGAATCCACTTAATAATTCAATTAATTCACTTCAAAATGAAATTGGACAATGG
GAACATGCTCTAAATAATAACAATAACGGAAATGACCCACTTGATCTTTGGTGGAAATAT
ATTTGCTGGCTTGAGAATCATCAAGAACTTGAAGCCAAATTTCGAAAAACGGTAGAGCAA
TGTCTTTCAATTTATGACAAGTATGAAATTTACAAACAAGATTTACGTCTTGTTAAACTT
TACATTAAATATATTGATTTTCAACCAAATCCTTTGAATTTATATAAAATTTTATATCAG
CAAGGCATAGGAACAAAATGTGCAGCATTTTACATTGGATGGGCACATTATTATAACTCT
GCAAATCATTTTAAACAAGCAGAATCTATATACAATTTGGGTGTACAATTGAAGGCTGAA
CCACTTAATGAATTGGAAACTGCTCAAAAGAATTTTCGCTTTTCTGTCGCACAACGGATG
CTTTATAATGATGAATCTTCAAAGAAGAGAACAATTTCGTCTCTTGAGGAGCAAAGACAA
CAAATAACATCGCTCTCTCCTCATCAAATACACTCAGCTAAAAGAATGAGAAGTGATT
>g9632.t2 Gene=g9632 Length=197
MNPLNNSINSLQNEIGQWEHALNNNNNGNDPLDLWWKYICWLENHQELEAKFRKTVEQCL
SIYDKYEIYKQDLRLVKLYIKYIDFQPNPLNLYKILYQQGIGTKCAAFYIGWAHYYNSAN
HFKQAESIYNLGVQLKAEPLNELETAQKNFRFSVAQRMLYNDESSKKRTISSLEEQRQQI
TSLSPHQIHSAKRMRSD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g9632.t2 | Gene3D | G3DSA:1.25.40.430 | - | 14 | 173 | 4.2E-43 |
| 4 | g9632.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 174 | 189 | - |
| 5 | g9632.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 174 | 197 | - |
| 2 | g9632.t2 | PANTHER | PTHR14030 | MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 | 8 | 184 | 1.5E-36 |
| 1 | g9632.t2 | Pfam | PF08311 | Mad3/BUB1 homology region 1 | 17 | 133 | 1.5E-34 |
| 7 | g9632.t2 | ProSiteProfiles | PS51489 | BUB1 N-terminal domain profile. | 18 | 182 | 39.465 |
| 3 | g9632.t2 | SMART | SM00777 | mad3_bub1_i | 11 | 133 | 1.5E-38 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007094 | mitotic spindle assembly checkpoint signaling | BP |
KEGG
Orthology
- This transcript has been annotated with K06637 (mitotic checkpoint serine/threonine-protein kinase BUB1 beta)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
