General information for g9632.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Mitotic checkpoint serine/threonine-protein kinase BUB1.
Parent gene
- The parent gene g9632 is located on chr_1: 4374072..4375489 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9632 | g9632.t3 | isoform | g9632.t3 | 4373126 | 4374072 |
| chr_1 | g9632 | g9632.t3 | exon | g9632.t3.exon1 | 4373126 | 4373138 |
| chr_1 | g9632 | g9632.t3 | exon | g9632.t3.exon2 | 4373204 | 4373451 |
| chr_1 | g9632 | g9632.t3 | cds | g9632.t3.CDS1 | 4373216 | 4373451 |
| chr_1 | g9632 | g9632.t3 | exon | g9632.t3.exon3 | 4373512 | 4373608 |
| chr_1 | g9632 | g9632.t3 | cds | g9632.t3.CDS2 | 4373512 | 4373608 |
| chr_1 | g9632 | g9632.t3 | exon | g9632.t3.exon4 | 4373668 | 4373837 |
| chr_1 | g9632 | g9632.t3 | cds | g9632.t3.CDS3 | 4373668 | 4373837 |
| chr_1 | g9632 | g9632.t3 | exon | g9632.t3.exon5 | 4373901 | 4374072 |
| chr_1 | g9632 | g9632.t3 | cds | g9632.t3.CDS4 | 4373901 | 4374072 |
| chr_1 | g9632 | g9632.t3 | TTS | g9632.t3 | 4374662 | 4374662 |
| chr_1 | g9632 | g9632.t3 | TSS | g9632.t3 | NA | NA |
Sequences
>g9632.t3 Gene=g9632 Length=700
GAATGCTGATATTCTTCCTGGGTTTATGACTATATCCGCTGCAGTCATTGCACCAAATGC
AAGTGTATTAATTTCGGAATTTTCTCAATATGGATCTTTATTAGACATTAATAACCGCAT
AAGAACGGCTACAACTAAAGTTATGCATGAAAGTTTAGTAATGCATTTTTCGAGTCAAAT
ACTTAATATTGTTGCACATTTGCACGACTGCAAAATCATTCATGCAGATATCAAACCCGA
TAACTGGCTATTAATGACATTACCCTCAGCAGATACTCATATTCCAAGCTTAAGGTTAAT
TGATTTTGGTTGTGCAATCGACATGACACTCTTTAAAGAAGGACAGGAATTTAGAAAGAT
AATTGAAACTGATGGTTTTACATGTGTTGAAATGCAAGAGGGTAGATTATGGTCATATCA
GACTGACTTATTTTGTGTCGCTGGTACAATACATGTAATGTTGTTTGGTGAATATATGCA
AGTGAATAAGAAATTCGGTCAATGGGAAATTAAACAAAAATTTCCTAGGTATCTTAATAA
AACTTTATGGACTGATATATTCTCGAAATTGCTGAACATAAAAGATAGTAATAACTTGCC
AAAATTACGTCAAATGAAAGCTCAAATTGATCGAGAAATAAATGACAACGAACTTGTTAA
ACATATTCGAACAGTTAGCAATCTTATAAGAAAACGCTAA
>g9632.t3 Gene=g9632 Length=224
MTISAAVIAPNASVLISEFSQYGSLLDINNRIRTATTKVMHESLVMHFSSQILNIVAHLH
DCKIIHADIKPDNWLLMTLPSADTHIPSLRLIDFGCAIDMTLFKEGQEFRKIIETDGFTC
VEMQEGRLWSYQTDLFCVAGTIHVMLFGEYMQVNKKFGQWEIKQKFPRYLNKTLWTDIFS
KLLNIKDSNNLPKLRQMKAQIDREINDNELVKHIRTVSNLIRKR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g9632.t3 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1 | 224 | 0.000 |
| 2 | g9632.t3 | PANTHER | PTHR14030:SF4 | BUB1 KINASE, ISOFORM A-RELATED | 2 | 209 | 0.000 |
| 3 | g9632.t3 | PANTHER | PTHR14030 | MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1 | 2 | 209 | 0.000 |
| 1 | g9632.t3 | Pfam | PF00069 | Protein kinase domain | 9 | 155 | 0.000 |
| 6 | g9632.t3 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 224 | 15.241 |
| 4 | g9632.t3 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 10 | 184 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0007094 | mitotic spindle assembly checkpoint signaling | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
