General information for g9675.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 5-aminolevulinate synthase, erythroid-specific, mitochondrial.
Parent gene
- The parent gene g9675 is located on chr_1: 4538494..4544251 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9675 | g9675.t1 | TTS | g9675.t1 | 4537617 | 4537617 |
| chr_1 | g9675 | g9675.t1 | isoform | g9675.t1 | 4537773 | 4539742 |
| chr_1 | g9675 | g9675.t1 | exon | g9675.t1.exon1 | 4537773 | 4537895 |
| chr_1 | g9675 | g9675.t1 | cds | g9675.t1.CDS1 | 4537773 | 4537895 |
| chr_1 | g9675 | g9675.t1 | exon | g9675.t1.exon2 | 4537956 | 4539536 |
| chr_1 | g9675 | g9675.t1 | cds | g9675.t1.CDS2 | 4537956 | 4539536 |
| chr_1 | g9675 | g9675.t1 | exon | g9675.t1.exon3 | 4539740 | 4539742 |
| chr_1 | g9675 | g9675.t1 | cds | g9675.t1.CDS3 | 4539740 | 4539742 |
| chr_1 | g9675 | g9675.t1 | TSS | g9675.t1 | 4539997 | 4539997 |
Sequences
>g9675.t1 Gene=g9675 Length=1707
ATGAATCAACTTGTTAAATCAACTTCAAGCCTAATCCGCACGGTGAGCAGCAAAATGCCT
TGCCCATTTTTGACGCGTTTGAGTTCAACTTATGTCCGCAATTATGCACCAATCCTTATG
AAGACCTACGGCAATCAATGCCCTGTCGTTGCTCGTGCAATTTCTCAATTGCAAAATGCC
GCTGGTGCTACAACTATCCAAAATCAAGACCGTAAATTGAGCTCAACTCAAACTGAACCA
ATTGCTGAGAATCAATGCCAAGCGCCACCATCATTGGCTGAAGTTGATTCGCATTTGAAA
GAAGTTAATGAAGCTCTTTTGCAAGATATTCCACAAAATCGCGAATCACAACCACTTTTT
GCTTATGAAGATTTCTTTCATGAACAAATCATGAAGAAAAAGCGTGATCATTCATATCGT
GTGTTCAAGAAAGTCAATCGTTTAGCTGGTCCTGGACAATTCCCAAAAGGTCTCGAATAT
GGCAATCGTCCAATTACTGTTTGGTGTTCAAACGACTACTTAGGCATGTCATCGCATCCT
GAAGTTAAAAATGCCGTGCATGAAGCCTTAGAAAAATACGGCGCTGGTGCTGGAGGTACC
CGTAACATTTCTGGAAATTCCATGATGCATGAAGCACTTGAAAAGCAATTGGCTGGCTTA
CATCAAAAGGAAGCAGCACTTGTCTTCTCATCATGCTATGTGGCAAATGATTCAACTCTT
TTCACTCTCGCTAAATTGCTACCTGGTTGTCATATCTTTTCTGATGCTGGAAATCACGCT
TCAATGATTCAAGGCATAAGAAATAGTGGTGTGCCAAAGCACATCTTTCGGCATAATGAT
CCTGATCATTTACGCGAACTTATCTCGAAAATTCCGAAAGAAGTGCCAAAAATTGTTGCT
TTCGAGACTGTGCATAGCATGACTGGCGCTGTCTGCCCTTTGGAAGAACTCTGTGATATC
GCTCATGAACATGGTGCTATTACATTTGTCGATGAAGTTCATGCCGTAGGTCTCTATGGT
GAACATGGTGCTGGAATTGGTGAACGTGATGGTATGATGCATAAAATGGACATTATTTCG
GGAACTTTGGGAAAAGCTTTTGGAAATGTTGGCGGATATATTGCATCGACAAGTAATTTG
GTTGATATGATTCGCTCTTATGCTGCTGGTTTTATTTTCACCACTTCGTTACCACCGACT
GTCTTGTGTGGTGCATCTAAAGCTGTTGAAGTTCTTGCATCAGAAGAAGGTCGTCAACTT
CGTGAAAAACATCAATCAAATGTTCGCTATTTACGCAATAAGTTGAAGGCTGAAGGTTTC
CCAGTTGAACACACTCCATCACATATCATTCCAGTTAAAATTGGCAATCCTAAACATAGC
ACTGCTATTTCTGATATGTTGATTCAGGAATATGGTCATTATATACAAGCTATCAACTAT
CCAACTGTTGCTCGTGGTGATGAAAAACTTCGTTTGGCTCCAACTCCTTATCACGTCCCA
GAAATGGCTGATGTTCTTGTTGCTGACATGAAAGAAGTCTGGAAGAAACTTGGACTGCAA
TTAAAGGGCATGCAATGCTCAAAGACATGCGAATTCTGCCGTAAACCATTACTTTTTGAC
TACTATGAATCACGTACTCGCAGCAATTCTCCCGATTTAGAATGCCAAGTTTCTGCATGC
CCACAAAAAGTCGCTGCAGTTCAATAA
>g9675.t1 Gene=g9675 Length=568
MNQLVKSTSSLIRTVSSKMPCPFLTRLSSTYVRNYAPILMKTYGNQCPVVARAISQLQNA
AGATTIQNQDRKLSSTQTEPIAENQCQAPPSLAEVDSHLKEVNEALLQDIPQNRESQPLF
AYEDFFHEQIMKKKRDHSYRVFKKVNRLAGPGQFPKGLEYGNRPITVWCSNDYLGMSSHP
EVKNAVHEALEKYGAGAGGTRNISGNSMMHEALEKQLAGLHQKEAALVFSSCYVANDSTL
FTLAKLLPGCHIFSDAGNHASMIQGIRNSGVPKHIFRHNDPDHLRELISKIPKEVPKIVA
FETVHSMTGAVCPLEELCDIAHEHGAITFVDEVHAVGLYGEHGAGIGERDGMMHKMDIIS
GTLGKAFGNVGGYIASTSNLVDMIRSYAAGFIFTTSLPPTVLCGASKAVEVLASEEGRQL
REKHQSNVRYLRNKLKAEGFPVEHTPSHIIPVKIGNPKHSTAISDMLIQEYGHYIQAINY
PTVARGDEKLRLAPTPYHVPEMADVLVADMKEVWKKLGLQLKGMQCSKTCEFCRKPLLFD
YYESRTRSNSPDLECQVSACPQKVAAVQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g9675.t1 | CDD | cd06454 | KBL_like | 163 | 514 | 0.0 |
| 6 | g9675.t1 | Gene3D | G3DSA:3.90.1150.10 | Aspartate Aminotransferase | 132 | 507 | 4.1E-162 |
| 7 | g9675.t1 | Gene3D | G3DSA:3.40.640.10 | - | 180 | 415 | 4.1E-162 |
| 2 | g9675.t1 | PANTHER | PTHR13693:SF57 | 5-AMINOLEVULINATE SYNTHASE | 67 | 533 | 1.2E-214 |
| 3 | g9675.t1 | PANTHER | PTHR13693 | CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE | 67 | 533 | 1.2E-214 |
| 1 | g9675.t1 | Pfam | PF00155 | Aminotransferase class I and II | 165 | 507 | 1.7E-77 |
| 4 | g9675.t1 | SUPERFAMILY | SSF53383 | PLP-dependent transferases | 120 | 518 | 9.87E-109 |
| 5 | g9675.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 18 | - |
| 8 | g9675.t1 | TIGRFAM | TIGR01821 | 5aminolev_synth: 5-aminolevulinic acid synthase | 121 | 521 | 2.4E-183 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0009058 | biosynthetic process | BP |
| GO:0033014 | tetrapyrrole biosynthetic process | BP |
| GO:0003870 | 5-aminolevulinate synthase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0030170 | pyridoxal phosphate binding | MF |
KEGG
Orthology
- This transcript has been annotated with K00643 (5-aminolevulinate synthase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
