General information for g9771.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Senecionine N-oxygenase.
Parent gene
- The parent gene g9771 is located on chr_1: 5109433..5110509 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9771 | g9771.t1 | isoform | g9771.t1 | 5109241 | 5110509 |
| chr_1 | g9771 | g9771.t1 | exon | g9771.t1.exon1 | 5109241 | 5110509 |
| chr_1 | g9771 | g9771.t1 | cds | g9771.t1.CDS1 | 5109241 | 5110509 |
| chr_1 | g9771 | g9771.t1 | TSS | g9771.t1 | 5109251 | 5109251 |
| chr_1 | g9771 | g9771.t1 | TTS | g9771.t1 | 5110681 | 5110681 |
Sequences
>g9771.t1 Gene=g9771 Length=1269
ATGAAAGTAACGGTGGTCGGTTGTGGTCCAAGTGGCTTGTGTGCTATTAAAAATTGTATT
TCTGAGGGATTTGAAGTTGTTGCATTTGAACAAACTTCAGAAATTGGCGGCGAATGGAAT
TCAAATTTAGAAATTGGTCCAAATGTTCACTCAAAAATTTATGAAGGACTTTTGACGAAT
TTTCCGAAAGAAATGATGGAATTTAGTGATTTTTCTTATGATGAAAATGTAACTGATTCT
TTTCTGACACCAGATAAGGTTCAAAATTATTTTCTAAAATATACTGAGAAGTTTGACTTA
AGAAAACACATAAAATTCAATCACAAAGTCATCAATGTTCAACCAATATCTGATAATCAA
TGGGAAATAATTGTTGAAGATTTAAAGAATGATGAATACAAAACAATAACATGTGATAAA
GTCTTTATTTGCATTGGAATTTCTGCACCTTGGATACCAAAAATTGAAGGTCTAAATGAT
TTTGAAGGTAAAATCATTCATTCTCGTGATTATCGTTCAACAAAGATGTTCGAAAATAAA
AAAGTCTTACTCATGGGAAGTGGAGTAAGTGCTTTAGATATGGTCATTGAAATTGACAAA
GTAGCTGAGAAAGTCATTTGGATAAATAATTTTAGACAAACTCATGGTGCACAAATAAAG
CTAGAACTGTCTGATAAAACTATTGATAAATCATCTACTGTCAAAAGGTTCACAAAAAGT
GGAGCAGAATTTGAAGATGGAACTTTTGAAGAATTTGAAATTATTGCTCTAGCTACAGGA
TATGATTTCACTTTTCCATTTTTATCAGTTGACTCTGGAATTTCAGTTTATGACAAATTA
GTTCAGCAACTTTATAAACAAATAATTAATGCAAATCGACCATCAATGGCTATCATTGGA
TTGCCTTTATTTTCAATAACAATGCCATTATTTGAGCTACAAATTAAATTCTGTCTTCAA
TATTGGTCAGGTAGAAAGAATTTTCCTTCAAAAGATGAAATGTTGAAAGTTATTCAAAAA
GATATTAAAGTTCTAAAAATTGACGAGAATGTTCACAAAGCACAATGTTTGGGACTAGCA
AGTGATGAAATTTATTACAATGATCTCGCACAGACAGCAGAAATTAAGCCACTTAAGCCA
GTTTTTATAAAAATGTGTAATTTTCAAGTGAAAAACTCATCGAAAAATTACAGAAATTTT
CGTGATTACGAATTTAAAATTTTGAATGATTTTGAATTTTCGATTGAATGTTTGACTAAA
GAAAAATAA
>g9771.t1 Gene=g9771 Length=422
MKVTVVGCGPSGLCAIKNCISEGFEVVAFEQTSEIGGEWNSNLEIGPNVHSKIYEGLLTN
FPKEMMEFSDFSYDENVTDSFLTPDKVQNYFLKYTEKFDLRKHIKFNHKVINVQPISDNQ
WEIIVEDLKNDEYKTITCDKVFICIGISAPWIPKIEGLNDFEGKIIHSRDYRSTKMFENK
KVLLMGSGVSALDMVIEIDKVAEKVIWINNFRQTHGAQIKLELSDKTIDKSSTVKRFTKS
GAEFEDGTFEEFEIIALATGYDFTFPFLSVDSGISVYDKLVQQLYKQIINANRPSMAIIG
LPLFSITMPLFELQIKFCLQYWSGRKNFPSKDEMLKVIQKDIKVLKIDENVHKAQCLGLA
SDEIYYNDLAQTAEIKPLKPVFIKMCNFQVKNSSKNYRNFRDYEFKILNDFEFSIECLTK
EK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g9771.t1 | Gene3D | G3DSA:3.50.50.60 | - | 2 | 403 | 0 |
| 16 | g9771.t1 | Gene3D | G3DSA:3.50.50.60 | - | 154 | 258 | 0 |
| 3 | g9771.t1 | PANTHER | PTHR23023 | DIMETHYLANILINE MONOOXYGENASE | 2 | 381 | 0 |
| 18 | g9771.t1 | PIRSF | PIRSF000332 | FMO | 1 | 217 | 0 |
| 17 | g9771.t1 | PIRSF | PIRSF000332 | FMO | 221 | 403 | 0 |
| 10 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 1 | 17 | 0 |
| 9 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 25 | 49 | 0 |
| 7 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 79 | 94 | 0 |
| 8 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 104 | 116 | 0 |
| 4 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 120 | 136 | 0 |
| 11 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 166 | 180 | 0 |
| 12 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 193 | 208 | 0 |
| 6 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 245 | 272 | 0 |
| 5 | g9771.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 309 | 322 | 0 |
| 2 | g9771.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 2 | 206 | 0 |
| 1 | g9771.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 231 | 379 | 0 |
| 14 | g9771.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 1 | 208 | 0 |
| 13 | g9771.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 234 | 411 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050661 | NADP binding | MF |
| GO:0050660 | flavin adenine dinucleotide binding | MF |
| GO:0004499 | N,N-dimethylaniline monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K00485 (dimethylaniline monooxygenase (N-oxide forming))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed