General information for g9772.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Senecionine N-oxygenase.
Parent gene
- The parent gene g9772 is located on chr_1: 5111063..5112397 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9772 | g9772.t1 | isoform | g9772.t1 | 5111063 | 5112397 |
| chr_1 | g9772 | g9772.t1 | exon | g9772.t1.exon1 | 5111063 | 5112397 |
| chr_1 | g9772 | g9772.t1 | cds | g9772.t1.CDS1 | 5111063 | 5112397 |
| chr_1 | g9772 | g9772.t1 | TSS | g9772.t1 | NA | NA |
| chr_1 | g9772 | g9772.t1 | TTS | g9772.t1 | NA | NA |
Sequences
>g9772.t1 Gene=g9772 Length=1335
ATGAAAACTAAAATTGCAGTGGTAGGTTGTGGCCCAAGTGGCTTGTGTGCTATTAAAAAT
TGCATTTCTGAGGGTTTTGAAGTTGTTGCATTTGAACAAACTTCAGAAATTGGTGGCGAA
TGGAATTCAAATTTAGAAGTTGGTCCAAATGTTCACTCAAAAATTTATGAAGGCTTGCAA
ATAAATTTTCCAAAAGAGCTTATGGAATTTTCTGATTTTTCTTATGATAAAAATATAACT
GAAGTTTTTCTGACACCAGATAAGGTTCAAAATTATTTTCTAAAATATACTGAAAAGTTT
GACTTAAGAAAGCACATAAAATTCAATCACAAAATCATCAATGTTCGACCAATTGATGAT
AAATGGGAAATAATAGTTAAAGATTTAAAGAATGACGAATACAAAACAATAACATGTGAT
AAAGTTCTAATTTGTGTTGGAATTTCCACTCCATGGATACCAAAAATTGAAGGTCTAAAT
GATTTTGAAGGCAAAATCATTCATTCTCGTGATTATCGTTCAACAAAGATGTTCGAAAAT
AAGAAAGTTTTACTCATTGGCAGTGGTTTCAGTGCATTTGATATAATAATTGCCATTGAT
AAAGTTGCAGAAAAAGTCACTTGGGTTAGAAAGATCAAAGAAGCTCATGCTCCATTTTTG
AAAATAAAACTTTCTTCTAAATCCACTGAAAAAACAATTCTAATTAAAAGGTTCACAAAA
AGTGGAGCAGAATTTGAAGATGGAAGTTTTGAAGAATTTGAAATTATTGCATTTGCAACA
GGGTATGATTTCACTTTTCCATTTTTATCAGTTGACTCTGGAATTTCAGTTTATGATAAA
TTAGTTCAACCACTTTATAAACAAATAATTAATGCAAATCGACCATCAATGGCCATTATC
GGTTTGCCTTCTTTTTCTCTTCGAATGCCAATGTTTGAATTACAGATAAAATTTTGTATG
CAATATTGGTCAAATAGGAAAATTTTTCCATCAAAAGAAGAAATGCTGGAAGACATCCAA
AAAGATTTTAAACTTCGAGGAGTTTATATTAATAGAAACAGTCACAATGCTCATTATTTA
GGACTGAAAAATCATGAATTTTACTATCAAGATTTAGCAGAAACAGCAGGAATTGCAAAT
ATAAAAATTTGTCTTATAAAAATGTCAACACATATAGGAAAACATACTTTCGAAAATTTT
CATACATTTCGCAATTTTAAATATAAAATTTTAAATGATTTTGAATTTTCATGTGATTGC
CTTGAAAACCAAAAAGATGAAAATGAAAGAAATCAGAATTGTGTGATAGCAAAAATGTTA
AAACTGTCTATTTGA
>g9772.t1 Gene=g9772 Length=444
MKTKIAVVGCGPSGLCAIKNCISEGFEVVAFEQTSEIGGEWNSNLEVGPNVHSKIYEGLQ
INFPKELMEFSDFSYDKNITEVFLTPDKVQNYFLKYTEKFDLRKHIKFNHKIINVRPIDD
KWEIIVKDLKNDEYKTITCDKVLICVGISTPWIPKIEGLNDFEGKIIHSRDYRSTKMFEN
KKVLLIGSGFSAFDIIIAIDKVAEKVTWVRKIKEAHAPFLKIKLSSKSTEKTILIKRFTK
SGAEFEDGSFEEFEIIAFATGYDFTFPFLSVDSGISVYDKLVQPLYKQIINANRPSMAII
GLPSFSLRMPMFELQIKFCMQYWSNRKIFPSKEEMLEDIQKDFKLRGVYINRNSHNAHYL
GLKNHEFYYQDLAETAGIANIKICLIKMSTHIGKHTFENFHTFRNFKYKILNDFEFSCDC
LENQKDENERNQNCVIAKMLKLSI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g9772.t1 | Coils | Coil | Coil | 411 | 431 | - |
| 13 | g9772.t1 | Gene3D | G3DSA:3.50.50.60 | - | 4 | 405 | 3.7E-102 |
| 14 | g9772.t1 | Gene3D | G3DSA:3.50.50.60 | - | 155 | 259 | 3.7E-102 |
| 3 | g9772.t1 | PANTHER | PTHR23023 | DIMETHYLANILINE MONOOXYGENASE | 3 | 377 | 1.6E-78 |
| 17 | g9772.t1 | PIRSF | PIRSF000332 | FMO | 1 | 214 | 1.0E-50 |
| 16 | g9772.t1 | PIRSF | PIRSF000332 | FMO | 224 | 408 | 1.2E-18 |
| 9 | g9772.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 3 | 19 | 2.1E-24 |
| 10 | g9772.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 56 | 74 | 2.1E-24 |
| 6 | g9772.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 106 | 118 | 2.1E-24 |
| 5 | g9772.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 167 | 181 | 2.1E-24 |
| 8 | g9772.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 194 | 209 | 2.1E-24 |
| 4 | g9772.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 246 | 273 | 2.1E-24 |
| 7 | g9772.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 310 | 323 | 2.1E-24 |
| 1 | g9772.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 3 | 207 | 2.4E-41 |
| 2 | g9772.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 235 | 356 | 1.9E-15 |
| 12 | g9772.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 1 | 211 | 4.53E-46 |
| 11 | g9772.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 243 | 349 | 3.24E-6 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050661 | NADP binding | MF |
| GO:0050660 | flavin adenine dinucleotide binding | MF |
| GO:0004499 | N,N-dimethylaniline monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K00485 (dimethylaniline monooxygenase (N-oxide forming))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed