General information for g9774.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Senecionine N-oxygenase.
Parent gene
- The parent gene g9774 is located on chr_1: 5116083..5117291 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9774 | g9774.t1 | isoform | g9774.t1 | 5116083 | 5117291 |
| chr_1 | g9774 | g9774.t1 | exon | g9774.t1.exon1 | 5116083 | 5116116 |
| chr_1 | g9774 | g9774.t1 | cds | g9774.t1.CDS1 | 5116083 | 5116116 |
| chr_1 | g9774 | g9774.t1 | exon | g9774.t1.exon2 | 5116177 | 5117291 |
| chr_1 | g9774 | g9774.t1 | cds | g9774.t1.CDS2 | 5116177 | 5117291 |
| chr_1 | g9774 | g9774.t1 | TSS | g9774.t1 | NA | NA |
| chr_1 | g9774 | g9774.t1 | TTS | g9774.t1 | NA | NA |
Sequences
>g9774.t1 Gene=g9774 Length=1149
ATGAAGCTTTGTATAATAGGCGCTGGTGCATCAGGTCTCTGTGCAATAAAGAATGCAATT
AATTATGGTTTTGAAGACATAACTGCTTATGAGCAAGCAAATGAGATAGGAGGAACTTGG
GTTTATACAGATGAAATTGGCAAGGATAAAATTGGTTTGGAAATTCATTCAAGCATGTAT
CAAGGACTACAGACAAATTTACCAAAAAAAATCATGCATTATCCTGACTTTCCATTTCCC
GAGCAAGAAAAATCGTACATAACAGCCGAGGAGGTACTGAGGTACTATCAATCATATGCT
GACACATTTAACTTGAGGAAATTCATTAAATTTGAGCATCATGTGGTTCGAGTGCGACCC
TTGAGTAAAAATAATGCGTGGGAGGTAATTGTGAAGGACTTGAAAAGTGGAGAATATAAT
TTAGTCGAATATGACGCAGTTCTCGTGTGTAATGGCCATTACAATTCCCCATTAATTCCA
TTTTATGAAGGTCAAGATCTTTTTGAAGGCAAACAAATGCATTCGCATGATTATCGAAAT
GCGCAGCCTTTTAAAAATGAACCTGTTTTAATTATCGGTGCTGGACCAAGTGGTCGTGAT
GCAGTGACAGATATTCAATATGTAGCGTCAAATGTCACATGGTCACATCACATGGAGAAA
CTCTCATTGACAAAATTTCGAGATAACGTTATCTGCAAACCCGACGTCTCAAAAATCACC
AAAGACGGTGCAATATTTGTCGATGGATCTTATCAGCAATTTTCAGTAATTATCTATTGC
ACTGGATATCGGTACACGTTTCCATTCTTATCAGTTGATTGTGGATTAACAGCAGTAAAT
AATTGTGTCGAGCCTCTCTATAAGCACTGCATCAATATTAAATATCCAACAATGGCAATA
ATTGGTCTTCCTAATTTTATCTGTCCTAATCAGATGTTTGATCTACAAGTGAAATTCTGT
TTAGAATTCATCTCAGGAAAAAGAAAGTTGCCAAATAAAGAAGAAATGATTGAGGATTTA
GAAAATGTAAAAAGGATTCAACTTGTCGATAGAGAATTACCAAGAAAGAAATTTCATCAT
TTGGGTGTTGGCTATCATGAGAGTTATTATGAGTCTCTCGCAAAAATAGCAAATATTAGA
GCCGATTAA
>g9774.t1 Gene=g9774 Length=382
MKLCIIGAGASGLCAIKNAINYGFEDITAYEQANEIGGTWVYTDEIGKDKIGLEIHSSMY
QGLQTNLPKKIMHYPDFPFPEQEKSYITAEEVLRYYQSYADTFNLRKFIKFEHHVVRVRP
LSKNNAWEVIVKDLKSGEYNLVEYDAVLVCNGHYNSPLIPFYEGQDLFEGKQMHSHDYRN
AQPFKNEPVLIIGAGPSGRDAVTDIQYVASNVTWSHHMEKLSLTKFRDNVICKPDVSKIT
KDGAIFVDGSYQQFSVIIYCTGYRYTFPFLSVDCGLTAVNNCVEPLYKHCINIKYPTMAI
IGLPNFICPNQMFDLQVKFCLEFISGKRKLPNKEEMIEDLENVKRIQLVDRELPRKKFHH
LGVGYHESYYESLAKIANIRAD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g9774.t1 | Gene3D | G3DSA:3.50.50.60 | - | 2 | 380 | 2.6E-131 |
| 14 | g9774.t1 | Gene3D | G3DSA:3.50.50.60 | - | 161 | 260 | 2.6E-131 |
| 3 | g9774.t1 | PANTHER | PTHR23023:SF264 | FLAVIN-CONTAINING MONOOXYGENASE | 2 | 374 | 9.1E-84 |
| 4 | g9774.t1 | PANTHER | PTHR23023 | DIMETHYLANILINE MONOOXYGENASE | 2 | 374 | 9.1E-84 |
| 21 | g9774.t1 | PIRSF | PIRSF000332 | FMO | 1 | 230 | 1.4E-50 |
| 20 | g9774.t1 | PIRSF | PIRSF000332 | FMO | 219 | 382 | 6.2E-17 |
| 10 | g9774.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 1 | 17 | 3.9E-25 |
| 6 | g9774.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 26 | 50 | 3.9E-25 |
| 5 | g9774.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 144 | 160 | 3.9E-25 |
| 9 | g9774.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 200 | 215 | 3.9E-25 |
| 7 | g9774.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 247 | 274 | 3.9E-25 |
| 8 | g9774.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 295 | 312 | 3.9E-25 |
| 1 | g9774.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 2 | 217 | 6.5E-42 |
| 2 | g9774.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 229 | 344 | 1.2E-13 |
| 16 | g9774.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 17 | g9774.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 18 | g9774.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 19 | g9774.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 19 | - |
| 15 | g9774.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 382 | - |
| 12 | g9774.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 1 | 221 | 4.05E-49 |
| 11 | g9774.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 230 | 344 | 1.25E-8 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050661 | NADP binding | MF |
| GO:0050660 | flavin adenine dinucleotide binding | MF |
| GO:0004499 | N,N-dimethylaniline monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed