General information for g9776.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Senecionine N-oxygenase.
Parent gene
- The parent gene g9776 is located on chr_1: 5121070..5122375 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9776 | g9776.t1 | TTS | g9776.t1 | 5120988 | 5120988 |
| chr_1 | g9776 | g9776.t1 | isoform | g9776.t1 | 5121070 | 5122375 |
| chr_1 | g9776 | g9776.t1 | exon | g9776.t1.exon1 | 5121070 | 5122289 |
| chr_1 | g9776 | g9776.t1 | cds | g9776.t1.CDS1 | 5121070 | 5122289 |
| chr_1 | g9776 | g9776.t1 | exon | g9776.t1.exon2 | 5122339 | 5122375 |
| chr_1 | g9776 | g9776.t1 | cds | g9776.t1.CDS2 | 5122339 | 5122375 |
| chr_1 | g9776 | g9776.t1 | TSS | g9776.t1 | 5122426 | 5122426 |
Sequences
>g9776.t1 Gene=g9776 Length=1257
ATGAAGAAACTTTGTGTCATAGGCGCGGGTGCTGCAGGTCTAGCGGCTGCCAAGAATGGA
TTAAAATTAAATTGCGATGTGACAGTTTTCGAACAATCGAATGCAGTAGGTGGCACGTGG
GTTTATACTGATGAGATAGGAAAAGACAAATATGGACTAGATGTGCATTCGAGCATGTAT
AAAGGACTTCATACGAATTTGCCTAAAGAAATTATGTCATTTCCCGATTTTCCCATTCCA
TATCAAGAGAAATCATATATTTTAGCTGAAGATATGTTGGCCTATTTAAATTTATATGCT
GATAACTTTAAGTTAAAAGAGAAAATAAAATTTGAGCATCACGTTGTTAGAGTTCGACCA
TTAATTGATGACACTTGGGAAGTTCTTGTGAGAGATTTAAAAATTGAAAAATATTTAATT
TACAATTTTGATTTGATTTTTATCTGCAATGGTCATTATCATACACCAATCATTCCATTT
TACGAAGGAAAAAATTTTTTTAAAGGCAAAATTCTTCATTCTCACGATTATCGATGTCCT
GATCCTTTTGAAAATGAAACTGTTTTAATTATTGGTGCTGGACCAAGTGGAATGGATCTG
GCGAATGAAATTTCAAAAAAAGCAACACGAGTCACACTTTCTCATCATTTGCCTGAACCA
CCAAAAACACAATTCAAAACAAATGTCGATCTAAAGCCGGATGTGGTTAAACTCACAGAA
GACGGAGCAGCTTTTGCTGATGGAACTTTAAATACTTTTTCTGTTGTGTTTTACTGTACT
GGCTATAAATATACTTTCCCATTCTTATCATTTGATAGTGGAATAACTTGTGATGATAAT
TTTGTGAGACCTCTGTACAAACACTGCATAAATATTAATCGACCAACGCTTGCATTCATT
GGTCTTCCATTTTATGTCTGTGCTACTCAAATGTTTGACTTACAAGCAAGATTTTGTCTT
ACTTTTATGACTGATAAGAAAAAAATTCCATCAAAAACTGAAATGATTGAAGATTCTGAA
AAAGAAATGAATAAAAGATGGTCAAAGGGATTTAAAAAGCATCAAGCTCATTTAATGGGA
TGTGATCAAGGCAAATATTATGAAGATTTATCAAAAACAGCTGAAATAGAACCATTAAAA
CCTGTAATTGCTAAATTACACAATTTTAGTTCATTGAGGTTTCTCGATGACTTGACAAAC
TTTAGACAAGACATTTTTCGCATTATTGATGATGAAACATTCATAAAAGTTCAATAA
>g9776.t1 Gene=g9776 Length=418
MKKLCVIGAGAAGLAAAKNGLKLNCDVTVFEQSNAVGGTWVYTDEIGKDKYGLDVHSSMY
KGLHTNLPKEIMSFPDFPIPYQEKSYILAEDMLAYLNLYADNFKLKEKIKFEHHVVRVRP
LIDDTWEVLVRDLKIEKYLIYNFDLIFICNGHYHTPIIPFYEGKNFFKGKILHSHDYRCP
DPFENETVLIIGAGPSGMDLANEISKKATRVTLSHHLPEPPKTQFKTNVDLKPDVVKLTE
DGAAFADGTLNTFSVVFYCTGYKYTFPFLSFDSGITCDDNFVRPLYKHCININRPTLAFI
GLPFYVCATQMFDLQARFCLTFMTDKKKIPSKTEMIEDSEKEMNKRWSKGFKKHQAHLMG
CDQGKYYEDLSKTAEIEPLKPVIAKLHNFSSLRFLDDLTNFRQDIFRIIDDETFIKVQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g9776.t1 | Gene3D | G3DSA:3.50.50.60 | - | 3 | 403 | 2.1E-132 |
| 13 | g9776.t1 | Gene3D | G3DSA:3.50.50.60 | - | 160 | 259 | 2.1E-132 |
| 3 | g9776.t1 | PANTHER | PTHR23023:SF208 | FLAVIN-CONTAINING MONOOXYGENASE | 3 | 414 | 5.1E-90 |
| 4 | g9776.t1 | PANTHER | PTHR23023 | DIMETHYLANILINE MONOOXYGENASE | 3 | 414 | 5.1E-90 |
| 21 | g9776.t1 | PIRSF | PIRSF000332 | FMO | 1 | 219 | 4.4E-51 |
| 20 | g9776.t1 | PIRSF | PIRSF000332 | FMO | 218 | 387 | 1.9E-19 |
| 9 | g9776.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 2 | 18 | 1.4E-27 |
| 7 | g9776.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 26 | 50 | 1.4E-27 |
| 8 | g9776.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 143 | 159 | 1.4E-27 |
| 10 | g9776.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 199 | 214 | 1.4E-27 |
| 6 | g9776.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 246 | 273 | 1.4E-27 |
| 5 | g9776.t1 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 294 | 311 | 1.4E-27 |
| 1 | g9776.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 2 | 215 | 8.9E-44 |
| 2 | g9776.t1 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 221 | 378 | 7.6E-18 |
| 16 | g9776.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 17 | g9776.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 18 | g9776.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 19 | g9776.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 15 | g9776.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 418 | - |
| 11 | g9776.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 1 | 225 | 3.31E-48 |
| 12 | g9776.t1 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 239 | 393 | 2.2E-11 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050661 | NADP binding | MF |
| GO:0050660 | flavin adenine dinucleotide binding | MF |
| GO:0004499 | N,N-dimethylaniline monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K00485 (dimethylaniline monooxygenase (N-oxide forming))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
