General information for g9776.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Senecionine N-oxygenase.
Parent gene
- The parent gene g9776 is located on chr_1: 5121070..5122375 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9776 | g9776.t2 | TTS | g9776.t2 | 5120988 | 5120988 |
| chr_1 | g9776 | g9776.t2 | isoform | g9776.t2 | 5121070 | 5122375 |
| chr_1 | g9776 | g9776.t2 | exon | g9776.t2.exon1 | 5121070 | 5122283 |
| chr_1 | g9776 | g9776.t2 | cds | g9776.t2.CDS1 | 5121070 | 5122283 |
| chr_1 | g9776 | g9776.t2 | exon | g9776.t2.exon2 | 5122363 | 5122375 |
| chr_1 | g9776 | g9776.t2 | cds | g9776.t2.CDS2 | 5122363 | 5122375 |
| chr_1 | g9776 | g9776.t2 | TSS | g9776.t2 | 5122426 | 5122426 |
Sequences
>g9776.t2 Gene=g9776 Length=1227
ATGAAGAAACTTTCGGCTGCCAAGAATGGATTAAAATTAAATTGCGATGTGACAGTTTTC
GAACAATCGAATGCAGTAGGTGGCACGTGGGTTTATACTGATGAGATAGGAAAAGACAAA
TATGGACTAGATGTGCATTCGAGCATGTATAAAGGACTTCATACGAATTTGCCTAAAGAA
ATTATGTCATTTCCCGATTTTCCCATTCCATATCAAGAGAAATCATATATTTTAGCTGAA
GATATGTTGGCCTATTTAAATTTATATGCTGATAACTTTAAGTTAAAAGAGAAAATAAAA
TTTGAGCATCACGTTGTTAGAGTTCGACCATTAATTGATGACACTTGGGAAGTTCTTGTG
AGAGATTTAAAAATTGAAAAATATTTAATTTACAATTTTGATTTGATTTTTATCTGCAAT
GGTCATTATCATACACCAATCATTCCATTTTACGAAGGAAAAAATTTTTTTAAAGGCAAA
ATTCTTCATTCTCACGATTATCGATGTCCTGATCCTTTTGAAAATGAAACTGTTTTAATT
ATTGGTGCTGGACCAAGTGGAATGGATCTGGCGAATGAAATTTCAAAAAAAGCAACACGA
GTCACACTTTCTCATCATTTGCCTGAACCACCAAAAACACAATTCAAAACAAATGTCGAT
CTAAAGCCGGATGTGGTTAAACTCACAGAAGACGGAGCAGCTTTTGCTGATGGAACTTTA
AATACTTTTTCTGTTGTGTTTTACTGTACTGGCTATAAATATACTTTCCCATTCTTATCA
TTTGATAGTGGAATAACTTGTGATGATAATTTTGTGAGACCTCTGTACAAACACTGCATA
AATATTAATCGACCAACGCTTGCATTCATTGGTCTTCCATTTTATGTCTGTGCTACTCAA
ATGTTTGACTTACAAGCAAGATTTTGTCTTACTTTTATGACTGATAAGAAAAAAATTCCA
TCAAAAACTGAAATGATTGAAGATTCTGAAAAAGAAATGAATAAAAGATGGTCAAAGGGA
TTTAAAAAGCATCAAGCTCATTTAATGGGATGTGATCAAGGCAAATATTATGAAGATTTA
TCAAAAACAGCTGAAATAGAACCATTAAAACCTGTAATTGCTAAATTACACAATTTTAGT
TCATTGAGGTTTCTCGATGACTTGACAAACTTTAGACAAGACATTTTTCGCATTATTGAT
GATGAAACATTCATAAAAGTTCAATAA
>g9776.t2 Gene=g9776 Length=408
MKKLSAAKNGLKLNCDVTVFEQSNAVGGTWVYTDEIGKDKYGLDVHSSMYKGLHTNLPKE
IMSFPDFPIPYQEKSYILAEDMLAYLNLYADNFKLKEKIKFEHHVVRVRPLIDDTWEVLV
RDLKIEKYLIYNFDLIFICNGHYHTPIIPFYEGKNFFKGKILHSHDYRCPDPFENETVLI
IGAGPSGMDLANEISKKATRVTLSHHLPEPPKTQFKTNVDLKPDVVKLTEDGAAFADGTL
NTFSVVFYCTGYKYTFPFLSFDSGITCDDNFVRPLYKHCININRPTLAFIGLPFYVCATQ
MFDLQARFCLTFMTDKKKIPSKTEMIEDSEKEMNKRWSKGFKKHQAHLMGCDQGKYYEDL
SKTAEIEPLKPVIAKLHNFSSLRFLDDLTNFRQDIFRIIDDETFIKVQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g9776.t2 | Gene3D | G3DSA:3.50.50.60 | - | 10 | 393 | 0 |
| 13 | g9776.t2 | Gene3D | G3DSA:3.50.50.60 | - | 150 | 249 | 0 |
| 3 | g9776.t2 | PANTHER | PTHR23023:SF208 | FLAVIN-CONTAINING MONOOXYGENASE | 18 | 404 | 0 |
| 4 | g9776.t2 | PANTHER | PTHR23023 | DIMETHYLANILINE MONOOXYGENASE | 18 | 404 | 0 |
| 15 | g9776.t2 | PIRSF | PIRSF000332 | FMO | 1 | 208 | 0 |
| 14 | g9776.t2 | PIRSF | PIRSF000332 | FMO | 206 | 378 | 0 |
| 8 | g9776.t2 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 16 | 40 | 0 |
| 5 | g9776.t2 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 133 | 149 | 0 |
| 9 | g9776.t2 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 189 | 204 | 0 |
| 6 | g9776.t2 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 236 | 263 | 0 |
| 7 | g9776.t2 | PRINTS | PR00370 | Flavin-containing monooxygenase (FMO) signature | 284 | 301 | 0 |
| 2 | g9776.t2 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 4 | 205 | 0 |
| 1 | g9776.t2 | Pfam | PF00743 | Flavin-binding monooxygenase-like | 211 | 368 | 0 |
| 11 | g9776.t2 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 4 | 215 | 0 |
| 10 | g9776.t2 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 227 | 383 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0050661 | NADP binding | MF |
| GO:0050660 | flavin adenine dinucleotide binding | MF |
| GO:0004499 | N,N-dimethylaniline monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K00485 (dimethylaniline monooxygenase (N-oxide forming))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
