General information for g9823.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Delta-aminolevulinic acid dehydratase.
Parent gene
- The parent gene g9823 is located on chr_1: 5395690..5396417 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9823 | g9823.t3 | TTS | g9823.t3 | 5395014 | 5395014 |
| chr_1 | g9823 | g9823.t3 | isoform | g9823.t3 | 5395121 | 5396417 |
| chr_1 | g9823 | g9823.t3 | exon | g9823.t3.exon1 | 5395121 | 5395452 |
| chr_1 | g9823 | g9823.t3 | cds | g9823.t3.CDS1 | 5395121 | 5395452 |
| chr_1 | g9823 | g9823.t3 | exon | g9823.t3.exon2 | 5395689 | 5396417 |
| chr_1 | g9823 | g9823.t3 | cds | g9823.t3.CDS2 | 5395689 | 5396205 |
| chr_1 | g9823 | g9823.t3 | TSS | g9823.t3 | 5396696 | 5396696 |
Sequences
>g9823.t3 Gene=g9823 Length=1061
ATGTCAGCGAAAAATAAACTTCACTCATCGCAATTTCATCCACTTTTGAGGAAGTTTAAT
GAGCAAAATGTTGACATATTACCCCATAATTTGATGTATCCAATTTTCATTGTGTAAGAA
TTTAGAATTCTCAATGAGCCAATTGTAAATTCATTTATTATAATAATTTATATTTCAGTG
AAGATGATTCGAGTCAACAAGTTATTCCTTCGATGCCAAGTGTTTATCGTTTTGGTTTAA
AAAAGCTCATTGATCATTTAACTCCTTTAGTTGAACTTGGATTGAAATCAATTCTTGTCT
TTGGTGTTATCGATACATTGACAAAAGATGAAACTGGCTCGAATGCTGATTCCAAGAAAA
ATCCTGTGATTAAAGCTCTTCCAATTTTGCGTAAAAAATTTCCTGAATTGCTCATTGCTT
GTGATGTTTGTCTTTGTCCTTATACGGATCATGGCCATTGCGGAATTATCGAGGACGGTG
TGATTAATAATGAGAAGAGCATTGAAAGAATTGCAAACATTGCGCTTGAGTATGCACGTC
ATGGAGCTCACATTGTCGCTCCCTCAGATATGATGGATAATCGTATCTTTGCTATCAAGT
CACGTCTCATAGAAAATGGAATGGAAAAAAATTGTTCTGTTCTTTCTTACTCGGCCAAGT
TTGCAAGTGGTTTTTATGGTCCATTTAGAGATGCTGCAAAATCAATTCCACAGCAAGGAG
ATCGAAAAACATATCAATTGCCTGCTGGCAGTCGTGGATTAGCAATACGTGCAGTAAGAC
GTGATGTTGAAGAAGGCGCTGACATGCTTATGGTTAAGCCTGGAATTTCTTATCTTGATA
TCGTGCGTGATGTCAAAAATGAATTTCCTGAATATCCAATGTTCATTTATCAAGTTTCTG
GCGAATATACAATGCTTGTTTATGGTGCCAAGGCTGGTGCATTTGACTTGAAAACCGTAC
TGACAGAAATTCTCGTTTCAATGAGACGTGCTGGAGTCGATGTAATTATCTCTTACTTTA
CACCAAATATTCTCGAATGGATGAAAGAAGGAAGCTTTTAA
>g9823.t3 Gene=g9823 Length=282
MPSVYRFGLKKLIDHLTPLVELGLKSILVFGVIDTLTKDETGSNADSKKNPVIKALPILR
KKFPELLIACDVCLCPYTDHGHCGIIEDGVINNEKSIERIANIALEYARHGAHIVAPSDM
MDNRIFAIKSRLIENGMEKNCSVLSYSAKFASGFYGPFRDAAKSIPQQGDRKTYQLPAGS
RGLAIRAVRRDVEEGADMLMVKPGISYLDIVRDVKNEFPEYPMFIYQVSGEYTMLVYGAK
AGAFDLKTVLTEILVSMRRAGVDVIISYFTPNILEWMKEGSF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g9823.t3 | Gene3D | G3DSA:3.20.20.70 | Aldolase class I | 1 | 280 | 1.3E-129 |
| 2 | g9823.t3 | PANTHER | PTHR11458:SF0 | DELTA-AMINOLEVULINIC ACID DEHYDRATASE | 1 | 279 | 4.1E-117 |
| 3 | g9823.t3 | PANTHER | PTHR11458 | DELTA-AMINOLEVULINIC ACID DEHYDRATASE | 1 | 279 | 4.1E-117 |
| 12 | g9823.t3 | PIRSF | PIRSF001415 | Porphbilin_synth | 1 | 280 | 1.3E-109 |
| 4 | g9823.t3 | PRINTS | PR00144 | Delta-aminolevulinic acid dehydratase signature | 74 | 88 | 1.9E-52 |
| 9 | g9823.t3 | PRINTS | PR00144 | Delta-aminolevulinic acid dehydratase signature | 104 | 123 | 1.9E-52 |
| 7 | g9823.t3 | PRINTS | PR00144 | Delta-aminolevulinic acid dehydratase signature | 143 | 162 | 1.9E-52 |
| 5 | g9823.t3 | PRINTS | PR00144 | Delta-aminolevulinic acid dehydratase signature | 195 | 211 | 1.9E-52 |
| 6 | g9823.t3 | PRINTS | PR00144 | Delta-aminolevulinic acid dehydratase signature | 221 | 236 | 1.9E-52 |
| 8 | g9823.t3 | PRINTS | PR00144 | Delta-aminolevulinic acid dehydratase signature | 251 | 270 | 1.9E-52 |
| 1 | g9823.t3 | Pfam | PF00490 | Delta-aminolevulinic acid dehydratase | 1 | 276 | 1.2E-112 |
| 13 | g9823.t3 | ProSitePatterns | PS00169 | Delta-aminolevulinic acid dehydratase active site. | 195 | 207 | - |
| 14 | g9823.t3 | SMART | SM01004 | ALAD_2 | 1 | 277 | 1.6E-129 |
| 10 | g9823.t3 | SUPERFAMILY | SSF51569 | Aldolase | 1 | 279 | 1.05E-110 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046872 | metal ion binding | MF |
| GO:0033014 | tetrapyrrole biosynthetic process | BP |
| GO:0003824 | catalytic activity | MF |
| GO:0004655 | porphobilinogen synthase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01698 (porphobilinogen synthase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
