General information for g9926.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial.
Parent gene
- The parent gene g9926 is located on chr_1: 6059491..6061368 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9926 | g9926.t3 | TTS | g9926.t3 | 6058670 | 6058670 |
| chr_1 | g9926 | g9926.t3 | isoform | g9926.t3 | 6058772 | 6061068 |
| chr_1 | g9926 | g9926.t3 | exon | g9926.t3.exon1 | 6058772 | 6058924 |
| chr_1 | g9926 | g9926.t3 | cds | g9926.t3.CDS1 | 6058772 | 6058924 |
| chr_1 | g9926 | g9926.t3 | exon | g9926.t3.exon2 | 6058986 | 6061068 |
| chr_1 | g9926 | g9926.t3 | cds | g9926.t3.CDS2 | 6058986 | 6060782 |
| chr_1 | g9926 | g9926.t3 | TSS | g9926.t3 | 6061350 | 6061350 |
Sequences
>g9926.t3 Gene=g9926 Length=2236
ATGCTCCGTGTTCCATTGACACGGGCCGTTTTGGCCCAATCTCAGCGAGCTGGAGTGTTG
GCTACATCAAAGAGAATGCAATCAAAAGCACCGGAAAAAGTCGAAGTGTTTATTGATGAT
CAGCCAGTAATGGTTTTACCAGGAACTACAGTTTTACAGGTATTTTTCTTATTCTTTTTC
TAATAAAATATATACAATAATATATTTTTAGGCAGCAGCACAAGTTGGAATTGAGATTCC
AAGATTTTGTTATCATGAACGTTTAGCTGTCGCAGGAAATTGCCGTATGTGCTTAGTTGA
AGTTGAAAAGAGTCCTAAACCAGTCGCTGCATGTGCAATGCCAGTCATGAAGGGATGGAG
AATCAAGACAAATTCAGAAATGACACGTCGTGCACGTGAAGGTGTCATGGAATTTCTTCT
TGCAAATCATCCACTTGATTGCCCAATTTGTGATCAAGGTGGTGAGTGCGATTTACAAGA
TCAAGCAATGTCATTCGGTTCAGATCGTTCACGTTTTACTGACATCGATCACGCCGGCAA
AAGAGCAGTTGAAGATAAAGATATTGGTCCATTAATAAAAACCACTATGACTCGTTGCAT
TCATTGTACACGTTGCATTAGATTTGCCTCAGAAGTTGCCGGAGTTGATGATTTGGGCAC
AACTGGAAGAGGCAATGACATGCAAGTAGGAACATATGTTGAGAAATTATTCCTCTCTGA
AATATCTGGAAATGTAATTGATTTGTGTCCAGTCGGTGCACTCACCAATAAACCATACAG
TTTTGTTGCTCGTCCATGGGAAATCAGAAGAATCGATAGTATCGATGTACTCGATGCACT
TGGTAGCAATATTACAGTAAATACAAGAACTGGTGAAGTATTGCGAATTCTTCCTCGTGA
AAATGAAGACATTAATGAAGAATGGCTCTCGGATAAATCGAGATTTGCTTGTGATGGATT
AAAACGTCAACGTTTAATTACACCAATGTTGCGAAAACCAGATGGTGAATTAGAAGCTGT
CGAATGGGAAACAGCTTTGATTGGTGTTGCTCAAGCTGTCAAAAATGCAGGAAGCAAAGT
TGCAGCAGTTGCTGGTAGTTTTGTCGATGCTGAACAACTTGTTGCAGTAAAAGATTTGTT
AAATCGTCTCGGATCAGAATTGCTTTGTACTGAGCAAAAGTTCCCAACATCTGATGGACT
ATCTGGAATAGATTTCAGATCAAATTACTTACTCAATTCATCAATTGCTGCATGCGAAGA
TGCCGATTTAGTTCTTTTAGTCGGCACAAATCCAAGATATGAAGCTCCATTATTAAACAC
ACGATTGCGCAAAGGCTATGTTCATAAAGAGCTTGATGTTGCAGTAATTGGTCCACAAAT
TGATTTGTCATATCGTCATGAGCATCTCGGTAGTAATGCATCAATTATTCAAGATATTGT
TAGTGGAAAACATCCCTTCGCTAAACGTTTAAAATCAGCTAAACGTCCATTAATAATTGT
TGGTGCTAATGCTCTTTCACGTAATGATGGCAAAGCCTTTTTGTCATCTCTTCACTCATT
CGCAAATCAATTGAAACCAGAAAATAGCGACTGGAAAGTTTGGAATGTACTCCAAACAAA
TGCTTCTCAGGTTGCAGCACTTGACATTGGCTACAAACCCGGTGTTGATGAAGTAATTGC
TGCCGGTCCAAAAGTTTTATTCCTTCTTGGTGCCGATAATGAGGCAATAAATAAGGATAA
TATTCCTAAAGATTGTTTTGTTGTATATCTTGGACATCATGGTGATAATGGTGCACCATT
ATCTCATGCCATCCTTCCTGGAGCAGCTTATACTGAAAAGCAAGCAACATATGTGAACAC
AGAAGGTCGTGCACAACAGACACTCATTGCAATCACACCACCTGGAATGGCTCGCGAAGA
TTGGAAAATTATTAGAGCATTGTCTGAAGTTATTGGAACATCTTTACCATATGATAATTT
GGATGAAATTAGAGACCGATTGGAAGATATTGCACCACATCTTGTTCGTTATGGACGTTT
GGAAGAAAACAACTTTTTCGCTCAAGCTGAGCAATTAGCAAAATCACAGTCAGTAGCGTT
CCAAGGTGAAATTGATGTCAAACAAAAATCATTAGCAGATTTCTATATGACAGATTCCAT
TACAAGAGCGTCGCCTACAATGGCAAAATGTGTGCAAGCCATTAAAAAGGGGCAAGGAAG
AGCATACACATATTAA
>g9926.t3 Gene=g9926 Length=649
MCLVEVEKSPKPVAACAMPVMKGWRIKTNSEMTRRAREGVMEFLLANHPLDCPICDQGGE
CDLQDQAMSFGSDRSRFTDIDHAGKRAVEDKDIGPLIKTTMTRCIHCTRCIRFASEVAGV
DDLGTTGRGNDMQVGTYVEKLFLSEISGNVIDLCPVGALTNKPYSFVARPWEIRRIDSID
VLDALGSNITVNTRTGEVLRILPRENEDINEEWLSDKSRFACDGLKRQRLITPMLRKPDG
ELEAVEWETALIGVAQAVKNAGSKVAAVAGSFVDAEQLVAVKDLLNRLGSELLCTEQKFP
TSDGLSGIDFRSNYLLNSSIAACEDADLVLLVGTNPRYEAPLLNTRLRKGYVHKELDVAV
IGPQIDLSYRHEHLGSNASIIQDIVSGKHPFAKRLKSAKRPLIIVGANALSRNDGKAFLS
SLHSFANQLKPENSDWKVWNVLQTNASQVAALDIGYKPGVDEVIAAGPKVLFLLGADNEA
INKDNIPKDCFVVYLGHHGDNGAPLSHAILPGAAYTEKQATYVNTEGRAQQTLIAITPPG
MAREDWKIIRALSEVIGTSLPYDNLDEIRDRLEDIAPHLVRYGRLEENNFFAQAEQLAKS
QSVAFQGEIDVKQKSLADFYMTDSITRASPTMAKCVQAIKKGQGRAYTY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g9926.t3 | CDD | cd02773 | MopB_Res-Cmplx1_Nad11 | 177 | 556 | 0.0 |
| 9 | g9926.t3 | Gene3D | G3DSA:3.10.20.740 | - | 1 | 77 | 5.2E-21 |
| 8 | g9926.t3 | Gene3D | G3DSA:3.30.70.20 | - | 84 | 162 | 1.1E-6 |
| 4 | g9926.t3 | PANTHER | PTHR11615 | NITRATE, FORMATE, IRON DEHYDROGENASE | 1 | 595 | 6.5E-158 |
| 5 | g9926.t3 | PANTHER | PTHR11615:SF6 | NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL | 1 | 595 | 6.5E-158 |
| 1 | g9926.t3 | Pfam | PF10588 | NADH-ubiquinone oxidoreductase-G iron-sulfur binding region | 37 | 76 | 4.0E-17 |
| 3 | g9926.t3 | Pfam | PF00384 | Molybdopterin oxidoreductase | 229 | 554 | 4.7E-65 |
| 2 | g9926.t3 | Pfam | PF09326 | NADH-ubiquinone oxidoreductase subunit G, C-terminal | 585 | 635 | 2.8E-14 |
| 11 | g9926.t3 | ProSitePatterns | PS00642 | Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. | 52 | 64 | - |
| 12 | g9926.t3 | ProSitePatterns | PS00643 | Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. | 103 | 113 | - |
| 15 | g9926.t3 | ProSiteProfiles | PS51839 | His(Cys)3-ligated-type [4Fe-4S] domain profile. | 32 | 71 | 16.387 |
| 16 | g9926.t3 | ProSiteProfiles | PS51669 | Prokaryotic molybdopterin oxidoreductases 4Fe-4S domain profile. | 173 | 229 | 18.835 |
| 13 | g9926.t3 | SMART | SM00929 | NADH_G_4Fe_4S_3_2 | 37 | 77 | 1.2E-20 |
| 7 | g9926.t3 | SUPERFAMILY | SSF54862 | 4Fe-4S ferredoxins | 29 | 169 | 4.18E-35 |
| 6 | g9926.t3 | SUPERFAMILY | SSF53706 | Formate dehydrogenase/DMSO reductase, domains 1-3 | 171 | 586 | 1.57E-84 |
| 14 | g9926.t3 | TIGRFAM | TIGR01973 | NuoG: NADH dehydrogenase (quinone), G subunit | 1 | 552 | 8.1E-196 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0016651 | oxidoreductase activity, acting on NAD(P)H | MF |
| GO:0042773 | ATP synthesis coupled electron transport | BP |
| GO:0055114 | NA | NA |
| GO:0051536 | iron-sulfur cluster binding | MF |
| GO:0008137 | NADH dehydrogenase (ubiquinone) activity | MF |
| GO:0016491 | oxidoreductase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
