General information for g9997.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein Malvolio.
Parent gene
- The parent gene g9997 is located on chr_1: 6488925..6489203 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9997 | g9997.t1 | TSS | g9997.t1 | 6485973 | 6485973 |
| chr_1 | g9997 | g9997.t1 | isoform | g9997.t1 | 6486145 | 6489203 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon1 | 6486145 | 6486309 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS1 | 6486145 | 6486309 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon2 | 6486766 | 6486792 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS2 | 6486766 | 6486792 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon3 | 6486866 | 6486875 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS3 | 6486866 | 6486875 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon4 | 6487198 | 6487453 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS4 | 6487198 | 6487453 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon5 | 6487506 | 6487789 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS5 | 6487506 | 6487789 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon6 | 6487870 | 6488243 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS6 | 6487870 | 6488243 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon7 | 6488425 | 6488542 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS7 | 6488425 | 6488542 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon8 | 6488604 | 6488861 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS8 | 6488604 | 6488861 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon9 | 6488925 | 6489090 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS9 | 6488925 | 6489090 |
| chr_1 | g9997 | g9997.t1 | exon | g9997.t1.exon10 | 6489188 | 6489203 |
| chr_1 | g9997 | g9997.t1 | cds | g9997.t1.CDS10 | 6489188 | 6489203 |
| chr_1 | g9997 | g9997.t1 | TTS | g9997.t1 | 6489490 | 6489490 |
Sequences
>g9997.t1 Gene=g9997 Length=1674
ATGGCTGAAAGTGATGAACGACATCACGATGAAAGTGATAGAACAGTTCCTTATATAAAT
TACGAGGACCATTTTGAAGATGAGGAGGATGATCTCGGTCCTGAATTCTCAACATATTTT
TCAGACCAAAAAGTTGAAATTCCAACATTTCCTGATGACGATAAAGTCGGATTCAGTTTT
AGAAAATTATGGGCATTTACTGCTTATCTCGATCCTGGAAATATTGAAAGCGATTTACAA
TCTGGCTTTGTTGCAAAATATAAATTGCTATGGGTTTTGATGTGGGCAACAATATTAGGC
TTAGTAATGCAACGTTTGGCTATGAGAATTGGTGTCGTTACTGGCCTTCATTTGGCTGAA
ATGTGCAATCGACAGTACAAGAAATTTCCTCGACTTATTTTATGGATTATGATAGAAATT
GCTATAATTGGTAGTGATATGCAGGAAGTTATAGGAACAGCAATTGCTATATATTTGCTA
TCAATGAAAGCTGTTCCAATTTATATCGGAGTCATAATCACAATTTTTGACACATTTACT
TTCCTTTTAATTGACAAATATGGACTTAGAAAATTAGAATTTTTCTTTGGATTTCTTATC
ACTGTTATGGCAGTCACATTTGGATATGAATTTTTTGTTGTGAAGCCAGAAATCACCGAA
ATAATCAAAGGAGCTGCAATTCCATGGTGTGCCGATTGTGATAGTAGAGCATTATTGCAA
GCTGTGGGAATTGTTGGAGCTGTTATTATGCCACACAATCTTTATCTTCATTCAGCTTTA
GTAAAATCAAGAAAAGTTGATCGACATCAGCCATCGAAAGTTAGAGAAGCCAATTATTAT
TTCTTCATTGAAGCATCTATTGCACTTTTTGTTTCGTTCTTGATCAACGTCTTTGTTGTT
GCTGTTTTTGCTCACGGTCTTAATGGAAAGACCAATGAGGATGTAGTTAATGAATGTTTT
AACAAGACAATTTATGATGAAGCCAAAGCAATTTTTACTGGTGATGAATTAGATGTCGAT
ATTTATAAAGGTGGAATATTTTTGGGATGTACATTTGGTATTGTTGCACTCTATGTGTGG
GCAATTGGGATTCTTGCTGCTGGACAGAGTTCTACTATGACAGGAACATATAGTGGACAA
TTTGCAATGGAAGGTTTTCTCAATCTGAAATGGTCTAGATGGCATCGTGTTCTTGTTACC
CGAATGATTGCAGTTGTTCCAACATTTGTAGTGGCATTTTATAGTGAAATTGAGCAAGTA
ACTAAAATGAATGACTACTTAAATGCAATTATGGCATTGCAACTTCCATTTGCAACAATT
CCAACTATTGCATTTTCATCATCGGTTGCAATTATGGGTGAATTCGTAAATGGCTTTTGG
AATAAACTCATATCAATTTTACTTAGCATCACAGTGATAGGAATCAATTTGACGTTTATA
ATTAGTCGTTTAGAGCTATCACATGTGTCAGGATGGTTGACAATCTTTATTGTAATATTC
TCTATTCTCTATGTAATTTTCTGTATATATTTGGTGATTCATATGATTTGCATGCTTAAA
CCCGATTCACGAATAGCGAGATCGGTATTTGCAAGAAGGTTTGTACTAAAAACGGCGAGC
TCATCAGCTGATGCTCATGGTGATAGTTCGGTTACAAGACCACTCGTTTATTAG
>g9997.t1 Gene=g9997 Length=557
MAESDERHHDESDRTVPYINYEDHFEDEEDDLGPEFSTYFSDQKVEIPTFPDDDKVGFSF
RKLWAFTAYLDPGNIESDLQSGFVAKYKLLWVLMWATILGLVMQRLAMRIGVVTGLHLAE
MCNRQYKKFPRLILWIMIEIAIIGSDMQEVIGTAIAIYLLSMKAVPIYIGVIITIFDTFT
FLLIDKYGLRKLEFFFGFLITVMAVTFGYEFFVVKPEITEIIKGAAIPWCADCDSRALLQ
AVGIVGAVIMPHNLYLHSALVKSRKVDRHQPSKVREANYYFFIEASIALFVSFLINVFVV
AVFAHGLNGKTNEDVVNECFNKTIYDEAKAIFTGDELDVDIYKGGIFLGCTFGIVALYVW
AIGILAAGQSSTMTGTYSGQFAMEGFLNLKWSRWHRVLVTRMIAVVPTFVVAFYSEIEQV
TKMNDYLNAIMALQLPFATIPTIAFSSSVAIMGEFVNGFWNKLISILLSITVIGINLTFI
ISRLELSHVSGWLTIFIVIFSILYVIFCIYLVIHMICMLKPDSRIARSVFARRFVLKTAS
SSADAHGDSSVTRPLVY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g9997.t1 | Hamap | MF_00221 | Divalent metal cation transporter MntH [mntH]. | 51 | 484 | 35.737087 |
| 2 | g9997.t1 | PANTHER | PTHR11706:SF40 | NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2 | 30 | 524 | 1.6E-209 |
| 3 | g9997.t1 | PANTHER | PTHR11706 | SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER | 30 | 524 | 1.6E-209 |
| 7 | g9997.t1 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 134 | 160 | 5.1E-84 |
| 11 | g9997.t1 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 164 | 183 | 5.1E-84 |
| 6 | g9997.t1 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 189 | 210 | 5.1E-84 |
| 9 | g9997.t1 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 241 | 264 | 5.1E-84 |
| 10 | g9997.t1 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 368 | 387 | 5.1E-84 |
| 8 | g9997.t1 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 396 | 413 | 5.1E-84 |
| 5 | g9997.t1 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 428 | 447 | 5.1E-84 |
| 1 | g9997.t1 | Pfam | PF01566 | Natural resistance-associated macrophage protein | 75 | 458 | 4.2E-115 |
| 32 | g9997.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 88 | - |
| 39 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 89 | 112 | - |
| 29 | g9997.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 113 | 131 | - |
| 42 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 132 | 159 | - |
| 35 | g9997.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 160 | 164 | - |
| 36 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 165 | 184 | - |
| 28 | g9997.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 185 | 195 | - |
| 45 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 196 | 214 | - |
| 30 | g9997.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 215 | 236 | - |
| 38 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 237 | 256 | - |
| 25 | g9997.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 257 | 276 | - |
| 43 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 277 | 304 | - |
| 34 | g9997.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 305 | 345 | - |
| 44 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 346 | 367 | - |
| 26 | g9997.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 368 | 397 | - |
| 46 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 398 | 417 | - |
| 31 | g9997.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 418 | 428 | - |
| 37 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 429 | 451 | - |
| 24 | g9997.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 452 | 462 | - |
| 41 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 463 | 481 | - |
| 33 | g9997.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 482 | 492 | - |
| 40 | g9997.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 493 | 517 | - |
| 27 | g9997.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 518 | 557 | - |
| 23 | g9997.t1 | TIGRFAM | TIGR01197 | nramp: metal ion transporter, metal ion (Mn2+/Fe2+) transporter (Nramp) family | 67 | 452 | 7.9E-154 |
| 18 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 91 | 113 | - |
| 16 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 133 | 155 | - |
| 12 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 165 | 184 | - |
| 15 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 191 | 213 | - |
| 22 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 237 | 259 | - |
| 21 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 280 | 302 | - |
| 20 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 345 | 367 | - |
| 14 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 397 | 415 | - |
| 17 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 430 | 452 | - |
| 19 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 459 | 481 | - |
| 13 | g9997.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 491 | 513 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0046873 | metal ion transmembrane transporter activity | MF |
| GO:0030001 | metal ion transport | BP |
KEGG
Orthology
- This transcript has been annotated with K21398 (natural resistance-associated macrophage protein 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
