General information for g9997.t12
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein Malvolio.
Parent gene
- The parent gene g9997 is located on chr_1: 6488925..6489203 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g9997 | g9997.t12 | TSS | g9997.t12 | 6485973 | 6485973 |
| chr_1 | g9997 | g9997.t12 | isoform | g9997.t12 | 6486145 | 6489203 |
| chr_1 | g9997 | g9997.t12 | exon | g9997.t12.exon1 | 6486145 | 6486334 |
| chr_1 | g9997 | g9997.t12 | cds | g9997.t12.CDS1 | 6486145 | 6486334 |
| chr_1 | g9997 | g9997.t12 | exon | g9997.t12.exon2 | 6487198 | 6487453 |
| chr_1 | g9997 | g9997.t12 | cds | g9997.t12.CDS2 | 6487198 | 6487453 |
| chr_1 | g9997 | g9997.t12 | exon | g9997.t12.exon3 | 6487506 | 6487789 |
| chr_1 | g9997 | g9997.t12 | cds | g9997.t12.CDS3 | 6487506 | 6487789 |
| chr_1 | g9997 | g9997.t12 | exon | g9997.t12.exon4 | 6487870 | 6488243 |
| chr_1 | g9997 | g9997.t12 | cds | g9997.t12.CDS4 | 6487870 | 6488243 |
| chr_1 | g9997 | g9997.t12 | exon | g9997.t12.exon5 | 6488425 | 6488542 |
| chr_1 | g9997 | g9997.t12 | cds | g9997.t12.CDS5 | 6488425 | 6488542 |
| chr_1 | g9997 | g9997.t12 | exon | g9997.t12.exon6 | 6488604 | 6488861 |
| chr_1 | g9997 | g9997.t12 | cds | g9997.t12.CDS6 | 6488604 | 6488861 |
| chr_1 | g9997 | g9997.t12 | exon | g9997.t12.exon7 | 6488925 | 6489090 |
| chr_1 | g9997 | g9997.t12 | cds | g9997.t12.CDS7 | 6488925 | 6489090 |
| chr_1 | g9997 | g9997.t12 | exon | g9997.t12.exon8 | 6489188 | 6489203 |
| chr_1 | g9997 | g9997.t12 | cds | g9997.t12.CDS8 | 6489188 | 6489203 |
| chr_1 | g9997 | g9997.t12 | TTS | g9997.t12 | 6489490 | 6489490 |
Sequences
>g9997.t12 Gene=g9997 Length=1662
ATGGCTGAAAGTGATGAACGACATCACGATGAAAGTGATAGAACAGTTCCTTATATAAAT
TACGAGGACCATTTTGAAGATGAGGAGGATGATCTCGGTCCTGAATTCTCAACATATTTT
TCAGACCAAAAAGTTGAAATTCCAACATTTCCTGATGACGATAAAGTAATTTTGAATATT
TTAACTCATACTTATCTCGATCCTGGAAATATTGAAAGCGATTTACAATCTGGCTTTGTT
GCAAAATATAAATTGCTATGGGTTTTGATGTGGGCAACAATATTAGGCTTAGTAATGCAA
CGTTTGGCTATGAGAATTGGTGTCGTTACTGGCCTTCATTTGGCTGAAATGTGCAATCGA
CAGTACAAGAAATTTCCTCGACTTATTTTATGGATTATGATAGAAATTGCTATAATTGGT
AGTGATATGCAGGAAGTTATAGGAACAGCAATTGCTATATATTTGCTATCAATGAAAGCT
GTTCCAATTTATATCGGAGTCATAATCACAATTTTTGACACATTTACTTTCCTTTTAATT
GACAAATATGGACTTAGAAAATTAGAATTTTTCTTTGGATTTCTTATCACTGTTATGGCA
GTCACATTTGGATATGAATTTTTTGTTGTGAAGCCAGAAATCACCGAAATAATCAAAGGA
GCTGCAATTCCATGGTGTGCCGATTGTGATAGTAGAGCATTATTGCAAGCTGTGGGAATT
GTTGGAGCTGTTATTATGCCACACAATCTTTATCTTCATTCAGCTTTAGTAAAATCAAGA
AAAGTTGATCGACATCAGCCATCGAAAGTTAGAGAAGCCAATTATTATTTCTTCATTGAA
GCATCTATTGCACTTTTTGTTTCGTTCTTGATCAACGTCTTTGTTGTTGCTGTTTTTGCT
CACGGTCTTAATGGAAAGACCAATGAGGATGTAGTTAATGAATGTTTTAACAAGACAATT
TATGATGAAGCCAAAGCAATTTTTACTGGTGATGAATTAGATGTCGATATTTATAAAGGT
GGAATATTTTTGGGATGTACATTTGGTATTGTTGCACTCTATGTGTGGGCAATTGGGATT
CTTGCTGCTGGACAGAGTTCTACTATGACAGGAACATATAGTGGACAATTTGCAATGGAA
GGTTTTCTCAATCTGAAATGGTCTAGATGGCATCGTGTTCTTGTTACCCGAATGATTGCA
GTTGTTCCAACATTTGTAGTGGCATTTTATAGTGAAATTGAGCAAGTAACTAAAATGAAT
GACTACTTAAATGCAATTATGGCATTGCAACTTCCATTTGCAACAATTCCAACTATTGCA
TTTTCATCATCGGTTGCAATTATGGGTGAATTCGTAAATGGCTTTTGGAATAAACTCATA
TCAATTTTACTTAGCATCACAGTGATAGGAATCAATTTGACGTTTATAATTAGTCGTTTA
GAGCTATCACATGTGTCAGGATGGTTGACAATCTTTATTGTAATATTCTCTATTCTCTAT
GTAATTTTCTGTATATATTTGGTGATTCATATGATTTGCATGCTTAAACCCGATTCACGA
ATAGCGAGATCGGTATTTGCAAGAAGGTTTGTACTAAAAACGGCGAGCTCATCAGCTGAT
GCTCATGGTGATAGTTCGGTTACAAGACCACTCGTTTATTAG
>g9997.t12 Gene=g9997 Length=553
MAESDERHHDESDRTVPYINYEDHFEDEEDDLGPEFSTYFSDQKVEIPTFPDDDKVILNI
LTHTYLDPGNIESDLQSGFVAKYKLLWVLMWATILGLVMQRLAMRIGVVTGLHLAEMCNR
QYKKFPRLILWIMIEIAIIGSDMQEVIGTAIAIYLLSMKAVPIYIGVIITIFDTFTFLLI
DKYGLRKLEFFFGFLITVMAVTFGYEFFVVKPEITEIIKGAAIPWCADCDSRALLQAVGI
VGAVIMPHNLYLHSALVKSRKVDRHQPSKVREANYYFFIEASIALFVSFLINVFVVAVFA
HGLNGKTNEDVVNECFNKTIYDEAKAIFTGDELDVDIYKGGIFLGCTFGIVALYVWAIGI
LAAGQSSTMTGTYSGQFAMEGFLNLKWSRWHRVLVTRMIAVVPTFVVAFYSEIEQVTKMN
DYLNAIMALQLPFATIPTIAFSSSVAIMGEFVNGFWNKLISILLSITVIGINLTFIISRL
ELSHVSGWLTIFIVIFSILYVIFCIYLVIHMICMLKPDSRIARSVFARRFVLKTASSSAD
AHGDSSVTRPLVY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g9997.t12 | Hamap | MF_00221 | Divalent metal cation transporter MntH [mntH]. | 51 | 480 | 34.930729 |
| 2 | g9997.t12 | PANTHER | PTHR11706:SF40 | NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2 | 61 | 520 | 2.0E-201 |
| 3 | g9997.t12 | PANTHER | PTHR11706 | SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER | 61 | 520 | 2.0E-201 |
| 10 | g9997.t12 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 130 | 156 | 4.9E-84 |
| 6 | g9997.t12 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 160 | 179 | 4.9E-84 |
| 9 | g9997.t12 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 185 | 206 | 4.9E-84 |
| 5 | g9997.t12 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 237 | 260 | 4.9E-84 |
| 8 | g9997.t12 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 364 | 383 | 4.9E-84 |
| 7 | g9997.t12 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 392 | 409 | 4.9E-84 |
| 11 | g9997.t12 | PRINTS | PR00447 | Natural resistance-associated macrophage protein signature | 424 | 443 | 4.9E-84 |
| 1 | g9997.t12 | Pfam | PF01566 | Natural resistance-associated macrophage protein | 71 | 454 | 4.1E-115 |
| 31 | g9997.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 84 | - |
| 38 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 85 | 108 | - |
| 24 | g9997.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 109 | 127 | - |
| 39 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 128 | 155 | - |
| 33 | g9997.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 156 | 160 | - |
| 40 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 161 | 180 | - |
| 29 | g9997.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 181 | 191 | - |
| 37 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 192 | 210 | - |
| 34 | g9997.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 211 | 232 | - |
| 44 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 233 | 252 | - |
| 28 | g9997.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 253 | 272 | - |
| 45 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 273 | 300 | - |
| 30 | g9997.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 301 | 341 | - |
| 46 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 342 | 363 | - |
| 26 | g9997.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 364 | 393 | - |
| 42 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 394 | 413 | - |
| 35 | g9997.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 414 | 424 | - |
| 36 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 425 | 447 | - |
| 25 | g9997.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 448 | 458 | - |
| 41 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 459 | 477 | - |
| 32 | g9997.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 478 | 488 | - |
| 43 | g9997.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 489 | 513 | - |
| 27 | g9997.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 514 | 553 | - |
| 23 | g9997.t12 | TIGRFAM | TIGR01197 | nramp: metal ion transporter, metal ion (Mn2+/Fe2+) transporter (Nramp) family | 63 | 448 | 3.3E-153 |
| 14 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 87 | 109 | - |
| 17 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 129 | 151 | - |
| 18 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 161 | 180 | - |
| 21 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 187 | 209 | - |
| 16 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 233 | 255 | - |
| 22 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 276 | 298 | - |
| 19 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 341 | 363 | - |
| 20 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 393 | 411 | - |
| 15 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 426 | 448 | - |
| 13 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 455 | 477 | - |
| 12 | g9997.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 487 | 509 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0046873 | metal ion transmembrane transporter activity | MF |
| GO:0030001 | metal ion transport | BP |
KEGG
Orthology
- This transcript has been annotated with K21398 (natural resistance-associated macrophage protein 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
