General information for g11018.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as RAC serine/threonine-protein kinase.
Parent gene
- The parent gene g11018 is located on chr_1: 13423510..13425801 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g11018 | g11018.t5 | isoform | g11018.t5 | 13422172 | 13425801 |
| chr_1 | g11018 | g11018.t5 | exon | g11018.t5.exon1 | 13422172 | 13422234 |
| chr_1 | g11018 | g11018.t5 | cds | g11018.t5.CDS1 | 13422172 | 13422234 |
| chr_1 | g11018 | g11018.t5 | exon | g11018.t5.exon2 | 13422298 | 13422751 |
| chr_1 | g11018 | g11018.t5 | cds | g11018.t5.CDS2 | 13422298 | 13422751 |
| chr_1 | g11018 | g11018.t5 | exon | g11018.t5.exon3 | 13422811 | 13422975 |
| chr_1 | g11018 | g11018.t5 | cds | g11018.t5.CDS3 | 13422811 | 13422975 |
| chr_1 | g11018 | g11018.t5 | exon | g11018.t5.exon4 | 13423039 | 13423190 |
| chr_1 | g11018 | g11018.t5 | cds | g11018.t5.CDS4 | 13423039 | 13423190 |
| chr_1 | g11018 | g11018.t5 | exon | g11018.t5.exon5 | 13423248 | 13423445 |
| chr_1 | g11018 | g11018.t5 | cds | g11018.t5.CDS5 | 13423248 | 13423445 |
| chr_1 | g11018 | g11018.t5 | exon | g11018.t5.exon6 | 13423509 | 13423668 |
| chr_1 | g11018 | g11018.t5 | cds | g11018.t5.CDS6 | 13423509 | 13423668 |
| chr_1 | g11018 | g11018.t5 | exon | g11018.t5.exon7 | 13424203 | 13424772 |
| chr_1 | g11018 | g11018.t5 | cds | g11018.t5.CDS7 | 13424203 | 13424576 |
| chr_1 | g11018 | g11018.t5 | exon | g11018.t5.exon8 | 13425608 | 13425801 |
| chr_1 | g11018 | g11018.t5 | TSS | g11018.t5 | NA | NA |
| chr_1 | g11018 | g11018.t5 | TTS | g11018.t5 | NA | NA |
Sequences
>g11018.t5 Gene=g11018 Length=1956
ATGCTTTCATGAAATACGTGATTATCGTATAAAGTTTTTTTTCACAAATATAAAAGTTTG
TGCAAAAATAAGAAAAAGTGAATTAAAACTAGTGATAAATAGTAAAATAATTTGCTCTTA
AACCTCTAAATTGCACTACAATTAAATTTGTGAATGCTTGTATTGAATTGTGTAGTCAAA
ATTTAAATAAAAAAGAACTTTCCATCAAAACAATAATATTGTAATTAATTGTGTATAAAG
AAGGAAATGTAAAATAATTGTTGAAAGAAAAGAAACTTATTGATTAAAAAGAAATATATT
CCACAGCATATTGAGAAATTAATATTATTTGCATCATCATCAATAGTTTGTTTTGGGCAA
TAAAGAAGATAAAAGAAAAGTTGAGGAAAAATGTCACTTGCATCAACATCAATTGATTCA
GGACAATTGCCTGATCTTGTATCACATAATATTGACGTCAACTCGCGTCAAATCATTAAA
GAAGGATGGTTATTTAAACGTGGTGAACATATAAAAAATTGGAGATCGCGCTATTTTATT
TTACGATCAGACGGCTCACTTGTAGGCTATAAGAATCGACCAGACGCTCAAATTCCAACT
GAGCCGTCCAATAATTTTACTGTTGCACGATGTCAAATCATGTCAGTTGATCGACCAAGA
CCTTATACATTTATAATCAGAGGCCTACAATGGACCACTGTCATCGAGCGAATGTTTTAT
GTCGAGACAGAGCATGAGAGAACCCAATGGGTTGAAGCTATACGTGTTGCAAACTGTTTG
AGCGAAATTGATCAACAAGAAGAGCCAGTAGCAGAATTGAATGATAATGATGATGTAGAA
ATGGCATCAATTGCGGAAGAAGAAGAATTGGATGAAAAACTCTCTCATCAAGGAACATCG
ACAGGAAGTGGAAAGAAGAAAATTACATTAGAGAATTTTGAATTTTTAAAAGTGCTTGGA
CGAGGGTCTTATGGCAAAGTAATTTTATGTCGGGAAAAATCTACAGCCAAGTTATATGCG
ATAAAAATCCTTAAAAAGGAAGTCATCATACAAAAGAGTGAAGTCGAACACACGATGGCT
GAAAATCGAGTTTTACGCAAGACAAATCATCCATTTTTAATTTCATTGCGTTATTCATTT
CGCACTAATGAGCGACTATGTTTTGTCATGCAGTATGTCAACGGCGGTGAATTATATTTT
CATCTGAGAAATGAAAAAATATTTTCAGAAGATCGTACTCGATTTTATGGAGCAGAAATC
ATTTCAGCACTTGGATATTTGCATTCGCACAACATTGTTTATCGTGATGTAAAATTAGAG
AATCTTTTACTTGATAAAGATGGTCATATAAAAATTGCAGATTTTGGTTTATGTAAGGAA
GATATTACATATGATAGAACAACGAATACATTTTGTGGAACACCTGAATATTTGGCACCA
GAAATGTTAGAAGACAACGATTATGGATTAGCAGTCGATTGGTGGGGCACGGGTGTCGTC
ATGTACGAAATGATGTGTGGTCAATTACCGTTTGATAATCGTGACCATGACATTTTATTC
AAGCAAATTATGATGGATGATGTAAAGTTTCGACGCCCTGTTTCTAGTGAAGCAAAATCA
CTTTTAACTGGATTACTTGAAAAAAGTCCCCAAAAGCGCCTCGGTGGTGGACCAGATGAT
GTTCGAGAAATAATGGTGCATCCTTTCTTTGCTAGCATCAATTGGTATGATTTAGTGAAT
AAGAAAATTATTCCACCGTTCAAGCCGCAAGTTACCTCAGATACTGATACACGCTATTTC
GATTCTGAATTTACGGGTGAGAGTGTCGAATTGACACCGCCTGATCCAGCTGTTCAATTA
CAATCAATTCAGGAAGAACCTCATTTTTCTCAATTCAGTTATCAAGCAATTGCTTCATTT
ATGAGCAACAATTCTGCATTAGTTTCATTACCATAG
>g11018.t5 Gene=g11018 Length=521
MSLASTSIDSGQLPDLVSHNIDVNSRQIIKEGWLFKRGEHIKNWRSRYFILRSDGSLVGY
KNRPDAQIPTEPSNNFTVARCQIMSVDRPRPYTFIIRGLQWTTVIERMFYVETEHERTQW
VEAIRVANCLSEIDQQEEPVAELNDNDDVEMASIAEEEELDEKLSHQGTSTGSGKKKITL
ENFEFLKVLGRGSYGKVILCREKSTAKLYAIKILKKEVIIQKSEVEHTMAENRVLRKTNH
PFLISLRYSFRTNERLCFVMQYVNGGELYFHLRNEKIFSEDRTRFYGAEIISALGYLHSH
NIVYRDVKLENLLLDKDGHIKIADFGLCKEDITYDRTTNTFCGTPEYLAPEMLEDNDYGL
AVDWWGTGVVMYEMMCGQLPFDNRDHDILFKQIMMDDVKFRRPVSSEAKSLLTGLLEKSP
QKRLGGGPDDVREIMVHPFFASINWYDLVNKKIIPPFKPQVTSDTDTRYFDSEFTGESVE
LTPPDPAVQLQSIQEEPHFSQFSYQAIASFMSNNSALVSLP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g11018.t5 | CDD | cd01241 | PH_PKB | 26 | 125 | 3.56166E-58 |
| 12 | g11018.t5 | CDD | cd05571 | STKc_PKB | 187 | 506 | 0.0 |
| 9 | g11018.t5 | Gene3D | G3DSA:2.30.29.30 | - | 22 | 139 | 1.4E-30 |
| 8 | g11018.t5 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 181 | 477 | 2.6E-132 |
| 10 | g11018.t5 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 267 | 461 | 2.6E-132 |
| 4 | g11018.t5 | PANTHER | PTHR24351:SF209 | NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE | 29 | 498 | 1.3E-235 |
| 5 | g11018.t5 | PANTHER | PTHR24351 | RIBOSOMAL PROTEIN S6 KINASE | 29 | 498 | 1.3E-235 |
| 2 | g11018.t5 | Pfam | PF00169 | PH domain | 29 | 125 | 5.4E-15 |
| 3 | g11018.t5 | Pfam | PF00069 | Protein kinase domain | 183 | 440 | 4.3E-74 |
| 1 | g11018.t5 | Pfam | PF00433 | Protein kinase C terminal domain | 461 | 504 | 2.9E-8 |
| 14 | g11018.t5 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 189 | 212 | - |
| 13 | g11018.t5 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 302 | 314 | - |
| 20 | g11018.t5 | ProSiteProfiles | PS50003 | PH domain profile. | 27 | 129 | 16.139 |
| 19 | g11018.t5 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 183 | 440 | 48.781 |
| 18 | g11018.t5 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 441 | 514 | 15.709 |
| 16 | g11018.t5 | SMART | SM00233 | PH_update | 28 | 131 | 4.0E-13 |
| 17 | g11018.t5 | SMART | SM00220 | serkin_6 | 183 | 440 | 1.8E-105 |
| 15 | g11018.t5 | SMART | SM00133 | pkinase_C_6 | 441 | 506 | 1.4E-19 |
| 6 | g11018.t5 | SUPERFAMILY | SSF50729 | PH domain-like | 24 | 127 | 1.02E-22 |
| 7 | g11018.t5 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 178 | 499 | 1.62E-96 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K04456 (RAC serine/threonine-protein kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
