Gene loci information

Transcript annotation

  • This transcript has been annotated as Heat shock 70 kDa protein cognate 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g945 g945.t1 TTS g945.t1 6999959 6999959
chr_3 g945 g945.t1 isoform g945.t1 7000043 7002234
chr_3 g945 g945.t1 exon g945.t1.exon1 7000043 7001764
chr_3 g945 g945.t1 cds g945.t1.CDS1 7000043 7001764
chr_3 g945 g945.t1 exon g945.t1.exon2 7002064 7002234
chr_3 g945 g945.t1 cds g945.t1.CDS2 7002064 7002234
chr_3 g945 g945.t1 TSS g945.t1 7002474 7002474

Sequences

>g945.t1 Gene=g945 Length=1893
ATGCCGAAAGTTGCTATTGGAATTGATCTCGGTACCACTTACTCATGTGTTGGCGTCTTT
CAACATGGAAAAGTTGATATTATTGCGAATGAAATGGGTAATAGAACAACACCGAGTTAT
GTTGCCTTCAATGATTCTGAAAGATTAGTTGGAGATGCAGCTAAAAATCAAGTTGCAATG
AATCCTAAAAATTCTGTTTTTGATGCAAAACGATTAATTGGAAGAAAATTTGATGATGAG
AAAATTCAAGCTGACATGAAGCATTGGCCATTTAAAGTTATTAATGATTGTGGTAAACCG
AAAATTGAAGTTGAATTTAAAGGTGAATGTAAGAGATTTGCACCTGAAGAAATTAGTTCG
ATGATTCTATCAAAGATGAGAGAAATTGCTGAGGCTTATTTAGGTCAGAAGATCAATGAT
GCAGTCATTACTGTCCCAGCTTACTTCAATGATTCACAACGTCAAGCAACGAAAGATGCT
GGTGCAATTTCAGGCCTTAACGTCTTAAGAATCATCAATGAGCCAACCGCTGCTGCTTTG
GCTTATGGTTTGGACAAAAATCTCAAAGGAGAGAAGAATGTCCTAATTTTTGACCTCGGT
GGTGGAACCTTTGATGTTTCAATTCTTTCTATTGATGAAGGTTCACTTTTTGAAGTAAAA
TCAACAGCAGGCGATACTCATCTAGGTGGTGAAGATTTTGATAATCGTCTTGTCAATCAT
TTTGCAGAAGAATTTAAGCGTAAATTCAAAAAAGATTTAAGTGGAAATCCTAGAGCATTG
AGACGATTAAGAACAGCATGCGAGCGAGCCAAGAGAACTCTTTCATCATCAACAGAAGCA
TCAATTGAAATTGATGCATTATATGAAGGTGTTGACTTTTACTCGAAAATTACTAGAGCT
CGCTTCGAAGAACTTAATATGGATTTGTTTAGATCAACATTGCAACCTGTTGAAAAAGCA
CTTAATGATGCTAAAATGGATAAGGGACAGATTAATGAAGTAGTCTTAGTTGGTGGATCA
ACTCGAATTCCTAAAGTTCAAAAAATGTTGCAAGATTTCTTTGGGGGAAAAGCTTTAAAT
TTTTCAATTAATCCTGATGAAGCAGTTGCCTATGGTGCAGCAATTCAAGCGGCTATTCTC
ACAGGAGATACAAGTTCACAAATTCAAGATGTTTTGCTTGTTGATGTCACTCCATTATCA
CTTGGAATTGAAACAGCTGGTGGAGTGATGAGCAAACTTGTTGAGAGAAATTCAAGAATT
CCATGCAAACAGCAACAAACTTTTACTACTTATTCTGACAATCAAAATGCTGTTACAATT
CAAGTATTTGAAGGTGAAAGAGCAATGACTAAAGATAATAATTTGCTTGGTACTTTCAAT
CTCACTGGAATTGCACCCGCACCACGTGGAATACCGAAAATTGAAGTCACATTTGATCTC
GATGCAAATGGAATTCTGAATGTTTCAGCTAAAGATATATCAACTGGTAAACAGGAAAAA
ATCACAATAAAGAATGACAAAGGTCGCCTGTCAAAAGCTGAAATCGATCGTATGCTCGCA
GATGCTGAAAAGTATCGTGAAGAAGATGAGAAACAACAGAAACGAATCCAAGCACGCAAT
CAATTTGAAAGTTATATTTTCGGATGCAAACAGGCAGTTGAGGAAGCACCTGCCGGAAGA
ATAACTGAAGAAGATAAAAAGATTGTTCGAGACAAATGTGCTTCAGAACTGTCATGGCTT
GATTCAAACACTCTTGCTGAAAAGGAAGAATTTGAGGATCGTATGAAGGAAACTCAAAAA
ATTTGTGGACCAATTATGGCCAAAATTCATCAACAAGGCGCAAATGGTCAACAGCAATCA
TCAGGCAAACCAACTGTTGAAGAAGTTGATTAA

>g945.t1 Gene=g945 Length=630
MPKVAIGIDLGTTYSCVGVFQHGKVDIIANEMGNRTTPSYVAFNDSERLVGDAAKNQVAM
NPKNSVFDAKRLIGRKFDDEKIQADMKHWPFKVINDCGKPKIEVEFKGECKRFAPEEISS
MILSKMREIAEAYLGQKINDAVITVPAYFNDSQRQATKDAGAISGLNVLRIINEPTAAAL
AYGLDKNLKGEKNVLIFDLGGGTFDVSILSIDEGSLFEVKSTAGDTHLGGEDFDNRLVNH
FAEEFKRKFKKDLSGNPRALRRLRTACERAKRTLSSSTEASIEIDALYEGVDFYSKITRA
RFEELNMDLFRSTLQPVEKALNDAKMDKGQINEVVLVGGSTRIPKVQKMLQDFFGGKALN
FSINPDEAVAYGAAIQAAILTGDTSSQIQDVLLVDVTPLSLGIETAGGVMSKLVERNSRI
PCKQQQTFTTYSDNQNAVTIQVFEGERAMTKDNNLLGTFNLTGIAPAPRGIPKIEVTFDL
DANGILNVSAKDISTGKQEKITIKNDKGRLSKAEIDRMLADAEKYREEDEKQQKRIQARN
QFESYIFGCKQAVEEAPAGRITEEDKKIVRDKCASELSWLDSNTLAEKEEFEDRMKETQK
ICGPIMAKIHQQGANGQQQSSGKPTVEEVD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
24 g945.t1 CDD cd10233 HSPA1-2_6-8-like_NBD 5 381 0.0
23 g945.t1 Coils Coil Coil 515 539 -
20 g945.t1 Gene3D G3DSA:3.30.420.40 - 6 378 9.7E-180
21 g945.t1 Gene3D G3DSA:3.30.30.30 - 67 113 9.7E-180
19 g945.t1 Gene3D G3DSA:3.30.420.40 - 187 365 9.7E-180
18 g945.t1 Gene3D G3DSA:3.90.640.10 Actin; Chain A 229 306 9.7E-180
17 g945.t1 Gene3D G3DSA:2.60.34.10 Substrate Binding Domain Of DNAk; Chain A 381 509 9.9E-61
22 g945.t1 Gene3D G3DSA:1.20.1270.10 - 510 612 3.7E-31
29 g945.t1 MobiDBLite mobidb-lite consensus disorder prediction 608 630 -
28 g945.t1 MobiDBLite mobidb-lite consensus disorder prediction 611 630 -
2 g945.t1 PANTHER PTHR19375 HEAT SHOCK PROTEIN 70KDA 4 617 0.0
3 g945.t1 PANTHER PTHR19375:SF431 HEAT SHOCK PROTEIN 68 4 617 0.0
7 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 4 17 7.9E-86
6 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 32 44 7.9E-86
8 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 54 62 7.9E-86
5 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 141 161 7.9E-86
10 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 202 212 7.9E-86
4 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 331 347 7.9E-86
11 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 363 383 7.9E-86
12 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 390 409 7.9E-86
9 g945.t1 PRINTS PR00301 70kDa heat shock protein signature 471 487 7.9E-86
1 g945.t1 Pfam PF00012 Hsp70 protein 5 610 1.7E-250
25 g945.t1 ProSitePatterns PS00297 Heat shock hsp70 proteins family signature 1. 8 15 -
27 g945.t1 ProSitePatterns PS00329 Heat shock hsp70 proteins family signature 2. 196 209 -
26 g945.t1 ProSitePatterns PS01036 Heat shock hsp70 proteins family signature 3. 334 348 -
16 g945.t1 SUPERFAMILY SSF53067 Actin-like ATPase domain 3 186 8.63E-71
15 g945.t1 SUPERFAMILY SSF53067 Actin-like ATPase domain 189 382 7.89E-68
14 g945.t1 SUPERFAMILY SSF100920 Heat shock protein 70kD (HSP70), peptide-binding domain 387 543 4.05E-61
13 g945.t1 SUPERFAMILY SSF100934 Heat shock protein 70kD (HSP70), C-terminal subdomain 537 615 8.37E-21

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005524 ATP binding MF
GO:0016887 ATP hydrolysis activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values